Vicilin and Napin Storage-Protein Gene Promoters Are Responsive to Abscisic Acid in Developing Transgenic Tobacco Seed but Lose Sensitivity following Premature Desiccation

Jiang, Liwen; Abrams, Suzanne R.; Kermode, Allison R.

doi:10.1104/pp.110.4.1135

Cited by 21 publications

(8 citation statements)

References 29 publications

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“…De novo synthesis of proteins associated with the defense against desiccation stress may have caused this increase. Synthesis of storage proteins is unlikely here because it was reported earlier that it ceases upon slow drying (Jiang et al, 1996). Proteins other than Leas also may be involved in the increase in ␣-helical structures.…”

Section: Discussionmentioning

confidence: 91%

Fourier Transform Infrared Microspectroscopy Detects Changes in Protein Secondary Structure Associated with Desiccation Tolerance in Developing Maize Embryos1

Wolkers

Bochicchio²,

Selvaggi³

et al. 1998

Plant Physiology

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Isolated immature maize (Zea mays L.) embryos have been shown to acquire tolerance to rapid drying between 22 and 25 d after pollination (DAP) and to slow drying from 18 DAP onward. To investigate adaptations in protein profile in association with the acquisition of desiccation tolerance in isolated, immature maize embryos, we applied in situ Fourier transform infrared microspectroscopy. In fresh, viable, 20-and 25-DAP embryo axes, the shapes of the different amide-I bands were identical, and this was maintained after flash drying. On rapid drying, the 20-DAP axes had a reduced relative proportion of ␣-helical protein structure and lost viability. Rapidly dried 25-DAP embryos germinated (74%) and had a protein profile similar to the fresh control axes. On slow drying, the ␣-helical contribution in both the 20-and 25-DAP embryo axes increased compared with that in the fresh control axes, and survival of desiccation was high. The protein profile in dry, mature axes resembled that after slow drying of the immature axes. Rapid drying resulted in an almost complete loss of membrane integrity in the 20-DAP embryo axes and much less so in the 25-DAP axes. After slow drying, low plasma membrane permeability ensued in both the 20-and 25-DAP axes. We conclude that slow drying of excised, immature embryos leads to an increased proportion of ␣-helical protein structures in their axes, which coincides with additional tolerance of desiccation stress.

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Section: Discussionmentioning

confidence: 91%

Fourier Transform Infrared Microspectroscopy Detects Changes in Protein Secondary Structure Associated with Desiccation Tolerance in Developing Maize Embryos1

Wolkers

Bochicchio²,

Selvaggi³

et al. 1998

Plant Physiology

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“…The effects of sense-bGlu I transformation and ABA on testa rupture of fresh seeds were not studied. The fresh tobacco seeds used in the earlier studies and in the present study were sampled approximately 40 DAP, which is after maturation and establishment of primary dormancy are completed (Yamaguchi-Shinozaki et al 1990;Jiang et al 1996;Phillips et al 1997). Reciprocal genetic crosses and the advantage that testa rupture and endosperm rupture are temporally separated events were utilized in the present study to assign bGlu I effects to either of the two covering layers.…”

Section: Discussionmentioning

confidence: 99%

Seed after-ripening and over-expression of class I β-1,3-glucanase confer maternal effects on tobacco testa rupture and dormancy release

Leubner‐Metzger

2002

Planta

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'Coat-imposed' seed dormancy of many non-endospermic and endospermic species is released during after-ripening. After-ripening-mediated promotion of tobacco ( Nicotiana tabacum L.) seed germination is mainly due to a promotion of testa rupture and a similar promotion of subsequent endosperm rupture. Treatment of after-ripened or freshly harvested mature seeds with abscisic acid (ABA) delays endosperm rupture and inhibits the induction of class I beta-1,3-glucanase (betaGlu I) in the micropylar endosperm, but does not affect the kinetics of testa rupture. After-ripening-mediated release of photodormancy is correlated with a decreased gibberellin (GA) requirement for testa rupture during dark-imbibition. Reciprocal crosses between wild-type tobacco and sense-betaGlu I transformant lines showed that betaGlu I over-expression in the seed covering layers can replace the promoting effect of after-ripening on testa rupture in light, but only if the mother plant is a sense-betaGlu I line. This maternal effect supports the model of two sites for betaGlu I action: (i) betaGlu I contribution to the after-ripening-mediated release of dormancy in the dry seed state, which is manifested in the promotion and ABA-insensitivity of testa rupture during imbibition. (ii) ABA-sensitive expression of betaGlu I in the micropylar endosperm, which contributes to endosperm rupture. The importance of GA-signaling and testa characteristics appear to be a common feature during the after-ripening-mediated release of coat-imposed dormancy in endospermic and non-endospermic seeds.

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“…2000 ; Koornneef & Karssen, 1994; Li & Foley, 1997; Rock & Quatrano, 1995). The onset of dormancy in tobacco is correlated with a peak in ABA content, which declines rapidly during further seed maturation (approximately 15–25 DAP) ( Jiang et al . 1996 ; Phillips et al .…”

Section: Discussionmentioning

confidence: 99%

Sense transformation reveals a novel role for class I β‐1,3‐glucanase in tobacco seed germination

Leubner‐Metzger¹,

Meins²

2000

The Plant Journal

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SummarỳCoat-enhanced' seed dormancy of many dicotyledonous species, including tobacco, is released during after-ripening. Rupture of the endosperm, which is the limiting step in tobacco seed germination, is preceded by induction of class I b-1,3-glucanase (bGLU I) in the micropylar endosperm where the radicle will penetrate. Treating after-ripened tobacco seeds with abscisic acid (ABA) delays endosperm rupture and inhibits bGLU I induction. Sense transformation with a chimeric ABA-inducible bGLU I transgene resulted in over-expression of bGLU I in seeds and promoted endosperm rupture of mature seeds and of ABA-treated after-ripened seeds. Taken together, these results provide direct evidence that bGLU I contributes to endosperm rupture. Over-expression of bGLU I during germination also replaced the effects of after-ripening on endosperm rupture. This suggests that regulation of bGLU I by ABA signalling pathways might have a key role in after-ripening.

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Vicilin and Napin Storage-Protein Gene Promoters Are Responsive to Abscisic Acid in Developing Transgenic Tobacco Seed but Lose Sensitivity following Premature Desiccation

Cited by 21 publications

References 29 publications

Fourier Transform Infrared Microspectroscopy Detects Changes in Protein Secondary Structure Associated with Desiccation Tolerance in Developing Maize Embryos1

Fourier Transform Infrared Microspectroscopy Detects Changes in Protein Secondary Structure Associated with Desiccation Tolerance in Developing Maize Embryos1

Seed after-ripening and over-expression of class I β-1,3-glucanase confer maternal effects on tobacco testa rupture and dormancy release

Sense transformation reveals a novel role for class I β‐1,3‐glucanase in tobacco seed germination

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