Vicilin from Anadenanthera colubrina Seeds: An alternative tool to combat Callosobruchus maculatus

Abstract

Highlights Vicilins from Anadenanthera colubrina seeds (AcVs) interfered with the development of cowpea weevil ( Callosobruchus maculatus ) larvae. AcVs present a strong effect at low concentrations on adult emergence (ED50 of 0.096%), on larvae, showing a marked reduction in mass (WD50 of 0.32%) and lethality (LD50 of 0.33%) (w:w). AcV is a chitin-binding protein with approximately 230 kDa. AcV chitin-binding fragments are associated with deleterious effects in larvae.

In-silico Studies Calculated a New Chitin Oligomer Binding Site Inside Vicilin: A Potent Antifungal and Insecticidal Agent

“… 26 VERIFY 3 D analysis and ERRAT score showed satisfactory and high quality of the predicted model. 39 Our site map analysis finds a new chitin-binding pocket in comparison to previously reported ones. Schrodinger site map analysis tool located site 4 at the junction of two monomers (chain A and chain C) and is residing at the front surface of the triangle.…”

“… 48 On the other hand, some authors have shown that cowpea vicilins cause detrimental effects on Callosobruchus maculatus larvae and these vicilin variants are not digested by the bruchid midgut proteinases. 39 Vicilins of various plants, such as Albizia lebbeck showed chitin-binding activity. 49 Another report has shown the vicilin binding to peritrophic membrane of Diatraea saccharalis and Tenebrio molitors , leading to inhibitory larval development.…”

In Silico Analysis and Functional Characterization of Antimicrobial and Insecticidal Vicilin from Moth Bean (Vigna aconitifolia (Jacq.) Marechal) Seeds

“…Previous findings suggest that vicilins are associated with fungal cell walls, possibly due to the chitin component in these structures [ 62 , 63 ]. Since chitin-binding proteins (vicilin) contain abundant positively charged residues (such as arginine), they could interact with the negatively charged components of the cell membrane, causing membrane disruption (depolarization of lipid membranes) and cell lysis (Figure S4) [ 64 ]. It is generally known that chitin found in fungal cell walls interacts with a group of proteins known as chitin binding proteins [ 20 , 53 ], causing growth inhibition in these species, as shown in [ 44 , 65 ].…”

“…Some proteins, such as vicilins, which have shown chitin binding affinity, which has been attributed to their insect toxicity [ 67 ]. It has been previously reported that vicilin extracted from a variety of legumes and plant seeds, including Phaseolus vulgaris , Phaseolus lunatus [ 22 ], Vigna angularis [ 35 ], Canavalia ensiformis [ 16 ], Glycine max [ 68 ], Vigna unguiculata [ 69 ], Enterolobium contortisiliquum [ 53 ], Anadenanthera colubrina [ 53 ] and Albizia lebbeck [ 63 ], showed insecticidal activity against Callosobruchus maculatus [ 70 ], Diatraea saccharalis [ 69 ], Tenebrio molitor [ 71 ], Plodia interpunctella [ 64 ], Ceratitis capitate [ 64 ] and Zabrotes subfasciatus [ 53 ], with a significant decrease in larval development and adult growth. Similarly, Vac V showed strong entomotoxic activity against the most harmful stored grains insects, S. oryzae .…”

Insecticidal and Detoxification Enzyme Inhibition Activities of Essential Oils for the Control of Pulse Beetle, Callosobruchus maculatus (F.) and Callosobruchus chinensis (L.) (Coleoptera: Bruchidae)

“…[ 6 ]. Callosobruchus maculatus and Callosobruchus chinensis are the major pest of cowpea and French beans in Africa and Asia which contains rich source of proteins (20-25%) and carbohydrates (50-60%) [ 7 , 8 ]. The grubs of the C. maculatus and C. chinensis feed on grains affecting quantitative and qualitative damage to the stored grains [ 9 ].…”