Victoria Blue B acts as a protein isomerization targeting probe for monitoring lysozyme fibrillation

Nie, Junlian; Tian, Pu; Chen, Zhijun

doi:10.1016/j.snb.2019.04.146

Cited by 6 publications

(4 citation statements)

References 34 publications

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“…However, the rotation of ThT is limited by the geometric constraints in the binding site and, thus, exhibits increased quantum yield after binding with fibrotic amyloid protein [ 32 , 33 ]. NR and VBB are also commercial specific probes for amyloid fibrils and show little changes toward monomeric proteins [ 34 , 35 , 36 , 37 ].…”

Section: Resultsmentioning

confidence: 99%

A Multichannel Fluorescent Tongue for Amyloid-β Aggregates Detection

Zhou

Gao

et al. 2022

IJMS

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Attention has been paid to the early diagnosis of Alzheimer’s disease, due to the maximum benefit acquired from the early-stage intervention and treatment. However, the sensing techniques primarily depended upon for neuroimaging and immunological assays for the detection of AD biomarkers are expensive, time-consuming and instrument dependent. Here, we developed a multichannel fluorescent tongue consisting of four fluorescent dyes and GO through electrostatic and π–π interaction. The array distinguished multiple aggregation states of 1 µM Aβ40/Aβ42 with 100% prediction accuracy via 10-channel signal outputs, illustrating the rationality of the array design. Screening vital sensor elements for the simplified sensor array and the optimization of sensing system was achieved by machine learning algorithms. Moreover, our sensing tongue was able to detect the aggregation states of Aβ40/Aβ42 in serum, demonstrating the great potential of multichannel array in diagnosing the Alzheimer’s diseases.

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Section: Resultsmentioning

confidence: 99%

A Multichannel Fluorescent Tongue for Amyloid-β Aggregates Detection

Zhou

Gao

et al. 2022

IJMS

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“…In our previous study, we have shown that VBB can function as a probe to monitor lysozyme fibrillation. [42] This property of VBB raised an intriguing question: does VBB influence the lysozyme fibrillation? To test this possibility, we carried out a series of experiments and found that VBB can inhibit lysozyme from forming fibrils.…”

Section: Vbb-fpns Inhibit the Lysozyme Fibrillationmentioning

confidence: 99%

Protein‐Based Nano‐Vessels Facilitates the Victoria Blue B Mediated Inhibition of Amyloid Fibrillation

Nie

Chen

2020

Macromol. Rapid Commun.

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“…[ 20–31 ] In particular, it has been reported to probe cellular viscosity distribution using confocal microscopy and fluorescence lifetime imaging (FLIM). [ 32–43 ] In 2018, Kuimova et al. designed a BODIPY‐based viscosity sensitivity probe for mapping microviscosity dynamics in cellular organelles.…”

Section: Introductionmentioning

confidence: 99%

“…[20][21][22][23][24][25][26][27][28][29][30][31] In particular, it has been reported to probe cellular viscosity distribution using confocal microscopy and fluorescence lifetime imaging (FLIM). [32][33][34][35][36][37][38][39][40][41][42][43] In 2018, Kuimova et al designed a BODIPY-based viscosity sensitivity probe for mapping microviscosity dynamics in cellular organelles. [44] Inspired by these pioneering work, we envision that conjugating an optimized BODIPY-based HaloTag probe to a HaloTag fused POI will allow us to study aggregation of POI using changes of both fluorescence intensity and lifetime.…”

Section: Introductionmentioning

confidence: 99%

A dual‐functional BODIPY‐based molecular rotor probe reveals different viscosity of protein aggregates in live cells

Shen

Jung

et al. 2022

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Aberrant protein aggregation leads to various human diseases, but little is known about the physical chemical properties of these aggregated proteins in cells. Herein, we developed a boron-dipyrromethene (BODIPY)-based HaloTag probe, whose conjugation to HaloTag-fused proteins allows us to study protein aggregates using both fluorescence intensity and lifetime. Modulation of BODIPY fluorophore reveals key structural features to attain the dual function. The optimized probe exhibits increased fluorescence intensity and elongated fluorescence lifetime in protein aggregates. Fluorescence lifetime imaging using this probe indicates that protein aggregates afford different viscosity in the forms of soluble oligomers and insoluble aggregates in live cells. The strategy presented in this work can be extended to enable a wide class of HaloTag probes that can be used to study a variety of physical properties of protein aggregates, thus helping unravel the pathogenic mechanism and develop therapeutic strategy.

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Victoria Blue B acts as a protein isomerization targeting probe for monitoring lysozyme fibrillation

Cited by 6 publications

References 34 publications

A Multichannel Fluorescent Tongue for Amyloid-β Aggregates Detection

A Multichannel Fluorescent Tongue for Amyloid-β Aggregates Detection

Protein‐Based Nano‐Vessels Facilitates the Victoria Blue B Mediated Inhibition of Amyloid Fibrillation

A dual‐functional BODIPY‐based molecular rotor probe reveals different viscosity of protein aggregates in live cells

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