Video-assisted orotracheal intubation in mice

Vergari, Alessandro; Polito, Angelo; Musumeci, Marco; Palazzesi, Sergio; Marano, Giuseppe

doi:10.1258/002367703766453047

Cited by 43 publications

(39 citation statements)

References 2 publications

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“…Also, the success rate was higher, and less importantly, the intubation time was at least 40% shorter by the microscopic method. A video-assisted orotracheal intubation, as also described in rats (Clary et al 2004), using a small fibreoptic arthroscope and a wire guide in mice, may be an alternative (Vergari et al 2003). The more difficult handling of a mouse lying supine may have translated to long intubation times of 3 + 0.4 min that virtually exclude inhalation anaesthesia.…”

Section: Discussionmentioning

confidence: 99%

Microscopic wire guide-based orotracheal mouse intubation: description, evaluation and comparison with transillumination

et al. 2008

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SummaryAirway access is needed for a number of experimental animal models, and the majority of animal research is based on mouse models. Anatomical conditions in mice are small, and the narrow glottic opening allows intubation only with a subtle technique. We therefore developed a microscopic endotracheal intubation method with a wire guide technique in mice anaesthetized with halothane in oxygen. The mouse is hung perpendicularly with its incisors on a thread fixed on a vertical plate. The tongue is placed with a pair of forceps between the left hand's thumb and forefinger and slightly pulled, while the neck and thorax are positioned using the third and fourth fingers. By doing so, the neck can be slightly stretched, which allows optimal visualization of the larynx and the vocal cords. To ensure a safe intubation, a fine wire guide is placed under vision between the vocal cords and advanced about 5 mm into the trachea. An intravenous 22G Â 1 in. plastic or Teflon catheter is guided over this wire. In a series of 41 mice, between 21 and 38 g, the success rate for the first intubation attempt was .95%. Certainty of the judgement procedure was 100% and success rate was higher using the described method when compared with a transillumination method in a further series. The technique is safe, less invasive than tracheostomy and suitable for controlled ventilation and pulmonary substance application.

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Section: Discussionmentioning

confidence: 99%

Microscopic wire guide-based orotracheal mouse intubation: description, evaluation and comparison with transillumination

et al. 2008

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“…Disease-free 7-to 8-wk-old C57BL/6 male mice (Jackson Laboratories, Bar Harbor, ME), weighing ϳ25 g each, were anesthetized with 120 -400 mg/kg Avertin (Sigma, St. Louis, MO) via intraperitoneal injection. Trans-oral intubation, thoracotomy, and excision of the left lung were performed as described previously (31,38). Briefly, trans-oral intubation was performed using a 2.7-mm zero degree rigid endoscope (Karl Storz, Charlton, MA) and an 18-gauge angiocatheter (Edwards Lifesciences, Irvine, CA) as an endotracheal tube.…”

Section: Methodsmentioning

confidence: 99%

Vascular endothelial growth factor accelerates compensatory lung growth after unilateral pneumonectomy

Sakurai¹,

Lee²,

Arsenault³

et al. 2007

American Journal of Physiology-Lung Cellular and Molecular Phys

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“…Many researchers have postulated various methods to carry out intubation of the mouse lung in order to assess both pulmonary function and BAL in the individual mice with longitudinal studies [19][20][21][22][23]. Such techniques appear to be largely successful but they are quite expensive and require intense training for the operator.…”

Section: Clinical Utility Of Balf Collectionmentioning

confidence: 99%

Aspiration techniques for bronchoalveolar lavage in translational respiratory research: Paving the way to develop novel therapeutic moieties

Dua

Shukla

Hansbro³

2017

J Biol Methods

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Bronchoalveolar lavage (BAL) is a simple, yet informative tool in understanding the immunopathology of various lung diseases via quantifying various inflammatory cells, cytokines and growth factors. At present, this traditional method is often blended with several robust and sophisticated molecular and biological techniques sustaining the significance and longevity of this technique. Crucially, the existence of slightly distinct approaches and variables employed at different laboratories around the globe in performing BAL aspiration indeed demands an utmost need to optimize and develop an effective, cost-effective and a reproducible technique. This mini review will be of importance to the biological translational scientist, particularly respiratory researchers in understanding the fundamentals and approaches to apply and consider with BAL aspiration techniques. This will ensure generating a meaningful and clinically relevant data which in turn accelerate the development of new and effective therapeutic moieties for major respiratory conditions.

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Video-assisted orotracheal intubation in mice

Cited by 43 publications

References 2 publications

Microscopic wire guide-based orotracheal mouse intubation: description, evaluation and comparison with transillumination

Microscopic wire guide-based orotracheal mouse intubation: description, evaluation and comparison with transillumination

Vascular endothelial growth factor accelerates compensatory lung growth after unilateral pneumonectomy

Aspiration techniques for bronchoalveolar lavage in translational respiratory research: Paving the way to develop novel therapeutic moieties

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