Video-rate dynamics of exocytotic events associated with phagocytosis in neutrophils

Suzaki, Etsuko; Kobayashi, Hideyuki; Kodama, Yuka; Masujima, Tsutomu; Terakawa, Susumu

doi:10.1002/(sici)1097-0169(1997)38:3<215::aid-cm1>3.0.co;2-4

Cited by 38 publications

(9 citation statements)

References 26 publications

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“…Bactericidal action of neutrophils in this case can be realized through secretion of reactive oxygen species and lytic enzymes in the extracellular medium, without phagosome formation. It is shown that in human neutrophils during phagocytosis of IgG-opsonized zymosan particles, secretion of specific and azurophilic granules precedes phagosome sealing, leading to the release of lysosomal enzymes such as h-glucuronidase in the extracellular medium [19,20].…”

Section: Discussionmentioning

confidence: 99%

Scanning electron microscopy study of neutrophil membrane tubulovesicular extensions (cytonemes) and their role in anchoring, aggregation and phagocytosis. The effect of nitric oxide

Galkina

Molotkovsky

Ullrich

et al. 2005

Experimental Cell Research

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Section: Discussionmentioning

confidence: 99%

Scanning electron microscopy study of neutrophil membrane tubulovesicular extensions (cytonemes) and their role in anchoring, aggregation and phagocytosis. The effect of nitric oxide

Galkina

Molotkovsky

Ullrich

et al. 2005

Experimental Cell Research

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“…Early observations by scanning electron microscopy revealed that the surface of macrophages becomes smoother after phagocytosis, suggesting that membrane folds have been flattened out to provide more membrane surface to the phagosome (184). In support of the second model, Hirsch and Cohn showed that neutrophils degranulate during phagocytosis and suggested that granules might fuse with the phagosome, which was later observed directly by video microscopy (185,186). Macrophage phagocytic capacity is reduced upon artificial expansion of the surface area of lysosomes or the depolymerization of microtubules, which are required for intracellular organelle movement, suggesting that mobilization of intracellular compartments contributes to the membrane reservoir (10).…”

Section: The Different Sources Of Membranementioning

confidence: 96%

Physical Constraints and Forces Involved in Phagocytosis

2020

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Phagocytosis is a specialized process that enables cellular ingestion and clearance of microbes, dead cells and tissue debris that are too large for other endocytic routes. As such, it is an essential component of tissue homeostasis and the innate immune response, and also provides a link to the adaptive immune response. However, ingestion of large particulate materials represents a monumental task for phagocytic cells. It requires profound reorganization of the cell morphology around the target in a controlled manner, which is limited by biophysical constraints. Experimental and theoretical studies have identified critical aspects associated with the interconnected biophysical properties of the receptors, the membrane, and the actin cytoskeleton that can determine the success of large particle internalization. In this review, we will discuss the major physical constraints involved in the formation of a phagosome. Focusing on two of the most-studied types of phagocytic receptors, the Fcγ receptors and the complement receptor 3 (αMβ2 integrin), we will describe the complex molecular mechanisms employed by phagocytes to overcome these physical constraints.

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“…Interestingly, in COS cells expressing hFc␥RIIA, Ca 2ϩ chelation impaired the delivery of lysosomal fluorescent dextran but not the transfer of endosomal markers, suggesting that only late fusion events are Ca 2ϩ -dependent [126]. In contrast, in neutrophils, fusion of primary and secondary granules occurs even before phagocytic cup closure [141,142], and these early fusion events can be blocked completely by Ca 2ϩ chelation [143][144][145].…”

Section: Ca 2؉ Dependence Of Phagosomal Maturationmentioning

confidence: 98%

The role of calcium signaling in phagocytosis

Nunes

Demaurex

2010

Journal of Leukocyte Biology

205

182

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Immune cells kill microbes by engulfing them in a membrane-enclosed compartment, the phagosome. Phagocytosis is initiated when foreign particles bind to receptors on the membrane of phagocytes. The best-studied phagocytic receptors, those for Igs (FcgammaR) and for complement proteins (CR), activate PLC and PLD, resulting in the intracellular production of the Ca(2+)-mobilizing second messengers InsP3 and S1P, respectively. The ensuing release of Ca(2+) from the ER activates SOCE channels in the plasma and/or phagosomal membrane, leading to sustained or oscillatory elevations in cytosolic Ca(2+) concentration. Cytosolic Ca(2+) elevations are required for efficient ingestion of foreign particles by some, but not all, phagocytic receptors and stringently control the subsequent steps involved in the maturation of phagosomes. Ca(2+) is required for the solubilization of the actin meshwork that surrounds nascent phagosomes, for the fusion of phagosomes with granules containing lytic enzymes, and for the assembly and activation of the superoxide-generating NADPH oxidase complex. Furthermore, Ca(2+) entry only occurs at physiological voltages and therefore, requires the activity of proton channels that counteract the depolarizing action of the phagocytic oxidase. The molecules that mediate Ca(2+) ion flux across the phagosomal membrane are still unknown but likely include the ubiquitous SOCE channels and possibly other types of Ca(2+) channels such as LGCC and VGCC. Understanding the molecular basis of the Ca(2+) signals that control phagocytosis might provide new, therapeutic tools against pathogens that subvert phagocytic killing.

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Video-rate dynamics of exocytotic events associated with phagocytosis in neutrophils

Cited by 38 publications

References 26 publications

Scanning electron microscopy study of neutrophil membrane tubulovesicular extensions (cytonemes) and their role in anchoring, aggregation and phagocytosis. The effect of nitric oxide

Scanning electron microscopy study of neutrophil membrane tubulovesicular extensions (cytonemes) and their role in anchoring, aggregation and phagocytosis. The effect of nitric oxide

Physical Constraints and Forces Involved in Phagocytosis

The role of calcium signaling in phagocytosis

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