Video-tracking and On-plant Tests Show Cry1Ab Resistance Influences Behavior and Survival of Neonate Ostrinia nubilalis Following Exposure to Bt Maize

Abstract

To examine how resistance to Bacillus thuringiensis (Bt) toxins influences movement and survival of European corn borer (Ostrinia nubilalis [Hübner]) neonates, the responses of Cry1Ab-resistant , -susceptible, and hybrid (F1) larvae were examined using two different techniques. First, using an automated videotracking system, aspects of O. nubilalis movement were quantified in the presence of artificial diet incorporating 50% non-Bt or insect-resistant Cry1Ab maize tissue. Second, O. nubilalis dispersal and survival were measured 48-72 h after hatching on a Cry1Ab maize plant surrounded by two non-Bt maize plants. Video tracking indicated the presence of Cry1Ab tissue increased the total distance moved (m), time moving (%), and time away from the diet (%) for O. nubilalis while decreasing meander (degrees/cm). However, resistant larvae showed reduced movement and increased meander (≈localized searching) relative to susceptible or hybrid larvae on diet This article is a U.S. government work, and is not subject to copyright in the United States.incorporating Cry1Ab tissue. Conversely, when placed onto Cry1Ab maize plants, resistant larvae were more likely than susceptible O. nubilalis to disperse onto adjacent non-Bt plants. The difference in on-plant dispersal seems to reflect greater survival after toxin exposure for resistant larvae rather than increased activity. These results suggest that simplified 'Petri dish' tests may not be predictive of larval movement among non-Bt and insect-resistant Bt maize plants. Because models of O. nubilalis resistance evolution incorporate various movement and survival parameters, improved data for on-plant behavior and survival of Bt-resistant , -susceptible, and hybrid larvae should help preserve the efficacy of transgenic insect-resistant maize.

Modeling the Impact of Cross-pollination and Low Toxin Expression in Corn Kernels on Adaptation of European Corn Borer (Lepidoptera: Crambidae) to Transgenic Insecticidal Corn

“…They studied movement and survival of three genotypes of larvae on one kind of transgenic corn. Prasifka et al (2009Prasifka et al ( , 2010 studied two genotypes of larvae under different conditions by using another transgenic insecticidal corn trait. Prasifka et al (2010) found that Cry1Ab resistant larvae had a higher probability of leaving a Bt corn plant for an adjacent non-Bt plant than did susceptible larvae.…”

“…Prasifka et al (2009Prasifka et al ( , 2010 studied two genotypes of larvae under different conditions by using another transgenic insecticidal corn trait. Prasifka et al (2010) found that Cry1Ab resistant larvae had a higher probability of leaving a Bt corn plant for an adjacent non-Bt plant than did susceptible larvae. Yet S. E. Moser et al (unpublished data) observed that susceptible larvae disperse from Cry1 Fexpressing corn more than Cry1 F-resistant larvae.…”

Updating risk management recommendations to limit exposure of non‐target Lepidoptera of conservation concern in protected habitats to Bt‐maize pollen

“…Pollen expression data compared with other studies are few and the mortality of the control is unacceptably high. In a further study, Xing et al (2008) observed that Bt-proteins in maize pollen were not detectable after 15 or 18 days, depending on the event Changes in feeding behaviour of lepidopteran larvae Scientific publications report examples of feeding inhibition, repellent effects and changes in feeding behaviour, particularly for Cry1F protein (Prasifka et al, 2007(Prasifka et al, , 2009Goldstein et al, 2010;Gaspers et al, 2011). In contrast, Halcomb et al (2000) observed no repellent effect on two lepidopteran species feeding on GM cotton expressing Only a limited number of lepidopteran species might be at risk.…”

Preference evaluation of stink bugs to leguminous seeds by video tracking system

“…노린재류의 집합페로몬은 먹이가 있는 경우 분비함 으로 (Morishima et al, 2005;Huh and Park, 2006), 집 합페로몬과 콩 종실을 조합하면 노린재류의 유인효과가 증 진한다고 하였다 (Huh and Park, 2006;Yasuda et al, 2007). 근년에 Video Tracking System(VTS)를 이용하여 곤충의 행동양상을 보다 정밀하게 관찰하는 연구가 활발하 게 이루어지고 있다 (Noldus et al, 2002;Prasifka et al, 2010) 에 차이가 있다 (Oh, 2008). …”