The growth and rapid development of virtual reality in the last decade has made its inclusion in classrooms of any stage of education to become a latent and patent reality. With the passing of time, virtual reality resulted in the creation of an augmented reality, which further advanced the immersive learning proposed by its predecessor. With technological growth, further advances in this technology were made, to what is known today as mixed reality, yet another step in the immersion of learning. The DIVEMIX project intends to bring to the table the transfer of the creation of materials based on this technology, to further develop the curriculum of the secondary education stage. Focusing our attention on the use of MR in the context of Biology and Geology teaching, and considering the current regulations in Spain as a frame of reference and starting point, we present the perceptions of pre-service secondary education teachers on the usability of this resource for teaching class content. The main result obtained was the lack of training and resources to be able to implement innovative actions in the classroom with MR. It was also determined that gender was not an element that determined the differences associated with the possession or not of knowledge that would allow teachers to use it in the classroom.