VIM-15 and VIM-16, Two New VIM-2-Like Metallo-β-Lactamases in
            <i>Pseudomonas aeruginosa</i>
            Isolates from Bulgaria and Germany

Schneider, Ines; Keuleyan, Emma; Raßhofer, R.; Markovska, Rumyana; Queenan, Anne Marie; Bauernfeind, A.

doi:10.1128/aac.00175-08

Cited by 29 publications

(19 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…retrograde cystourethrography and pouchography). Although MBL-producing P. aeruginosa isolates have been sporadically reported in Germany [13][14][15], to our knowledge, outbreaks have not yet been described.…”

Section: Introductionmentioning

confidence: 64%

Nosocomial outbreak of VIM-2 metallo-β-lactamase-producing Pseudomonas aeruginosa associated with retrograde urography

Elias

Schoen

Heinze

et al. 2010

Clinical Microbiology and Infection

View full text Add to dashboard Cite

Pseudomonas aeruginosa is well adapted to the hospital setting and can cause a wide array of nosocomial infections that occasionally culminate in recalcitrant outbreaks. In the present study, we describe the first nosocomial outbreak of infection caused by bla(VIM-2)-positive P. aeruginosa in Germany. In November and December 2007, highly resistant P. aeruginosa isolates were recovered from the urine of 11 patients in the Department of Urology of a University Hospital. Bacterial isolates were typed by multilocus sequence typing and screened for known metallo-β-lactamase (MBL) genes by PCR. Environmental sources of transmission were tested for bacterial contamination using surveillance cultures. Furthermore, a matched case-control study was performed in search of medical procedures significantly associated with case status. Typing of recovered isolates confirmed VIM-2 MBL-producing P. aeruginosa of sequence type 175 in all cases. Surveillance cultures did not lead to the identification of an environmental source of the outbreak strain. Case-control analysis revealed retrograde urography as the only exposure significantly associated with case status. The analyses suggest the transmission of a single clone of VIM-2 MBL-producing P. aeruginosa leading to the infection of 11 patients within 47 days. Events in temporal proximity to retrograde urographies appear to have facilitated infection in the majority of cases. Department-specific infection control measures, including reinforced hygiene procedures during retrograde urography, quickly terminated the outbreak.

show abstract

Section: Introductionmentioning

confidence: 64%

Nosocomial outbreak of VIM-2 metallo-β-lactamase-producing Pseudomonas aeruginosa associated with retrograde urography

Elias

Schoen

Heinze

et al. 2010

Clinical Microbiology and Infection

View full text Add to dashboard Cite

show abstract

“…Metallo-enzyme producing Pseudomonas are not typical for our country. The fi rst case of VIM-positive P. aeruginosa was published in 2008 by Keuleyan et al 23 Since then till 2015 no other cases have been reported even though VIM and/or IMP encoding strains have been determined in all our neighbouring countries -Greece, Serbia, Romania and Turkey except Macedonia. [24][25][26][27][28][29] In 2013 collaboration with the Medical University of Palma de Mayorca in Spain.…”

Section: Discussionmentioning

confidence: 99%

Carbapenemase Production of Clinical Isolates Acinetobacter baumannii and Pseudomonas aeruginosa from a Bulgarian University Hospital

Petrova¹,

Stanimirova²,

Ivanov³

et al. 2017

Folia Medica

View full text Add to dashboard Cite

Background: Production of Bla OXA-23, OXA-24, OXA-58 and hyperexpression of OXA-51 due to ISAba1 insertion sequence are the leading causes of carbapenem resistance in Acinetobacter baumannii. The loss of OprD transmembrane protein and the overexpression of some effl ux pumps are considered to be the main factors for carbapenem resistance in Pseudomonas aeruginosa whereas metallo-enzymes' production has a secondary role. Aim: Тo examine the carbapenem resistance due to carbapenemase production among clinically signifi cant Gram-negative non-fermenters from St George University hospital, Plovdiv: A. baumannii and P. aeruginosa. Materials and methods: Forty three A. baumannii and 43 P. aeruginosa isolates, resistant or with intermediate resistance to imipenem and/or meropenem were included in the study. They were collected from patients admitted in 14 various hospital wards between 2010 and 2014. Both phenotypic and genetic methods were used for identifi cation and antimicrobial susceptibility testing. Results: All A. baumannii demonstrated carbapenemase production determined by a modifi ed Hodge test whereas P. aeruginosa isolates did not show this phenomenon. OXA-23 genes were determined in 97.7% (42 out of 43) of A. baumannii isolates indistinguishable from the sequence of the classical ARI-1 gene. OXA-24, OXA-58 and overexpression of OXA-51 were not registered in any of the isolates. All P. aeruginosa were negative for blaVIM and blaIMP genes. Conclusion:The leading cause of carbapenem resistance in A. baumannii isolates from our hospital is the carbapenemase production due to the expression of OXA-23 gene, whereas in P. aeruginosa -the loss of transmembrane OprD protein and the effl ux pumps' hyperexpression are suspected to be the main mechanisms.

show abstract

“…Another B1 group that exhibits a similar number of variants is that of the VIM enzymes. Twelve VIM variants have been described to date, designated VIM-1 to VIM-16 [6,[34][35][36][37][38][39][40]109]. Genes encoding the IMP and VIM enzymes are found on mobile genetic elements, which facilitate their horizontal transference between different genera and species.…”

Section: Introductionmentioning

confidence: 99%

Outsmarting metallo-β-lactamases by mimicking their natural evolution

Oelschlaeger

2008

Journal of Inorganic Biochemistry

View full text Add to dashboard Cite

VIM-15 and VIM-16, Two New VIM-2-Like Metallo-β-Lactamases in Pseudomonas aeruginosa Isolates from Bulgaria and Germany

Cited by 29 publications

References 11 publications

Nosocomial outbreak of VIM-2 metallo-β-lactamase-producing Pseudomonas aeruginosa associated with retrograde urography

Nosocomial outbreak of VIM-2 metallo-β-lactamase-producing Pseudomonas aeruginosa associated with retrograde urography

Carbapenemase Production of Clinical Isolates Acinetobacter baumannii and Pseudomonas aeruginosa from a Bulgarian University Hospital

Outsmarting metallo-β-lactamases by mimicking their natural evolution

Contact Info

Product

Resources

About