Vimentin expression of esophageal squamous cell carcinoma and its aggressive potential for lymph node metastasis

Jin, Hiroyuki; Morohashi, Satoko; Sato, Fuyuki; Kudo, Yasuyuki; Akasaka, Harue; Tsutsumi, Shinji; Ogasawara, Hiroshi; Miyamoto, Keiichi; Wajima, Naoki; Kawasaki, Hitoshi; Hakamada, Kenichi; Kijima, Hiroshi

doi:10.2220/biomedres.31.105

Cited by 56 publications

(35 citation statements)

References 33 publications

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“…This is consistent with the established roles of EMT during ESCC progression, since reduced E-cadherin [16–20] with increased N-cadherin [21, 22] and vimentin [23, 24] expression would reduce cell adhesion, increase cell mobility, and ultimately enhance cell invasiveness and metastasis to form advanced stage tumors. However, we found no significant association between expression of any EMT marker in PTs and N-stage, a clinical lymph node metastasis parameter.…”

Section: Discussionsupporting

confidence: 85%

The epithelial-mesenchymal transition phenotype of metastatic lymph nodes impacts the prognosis of esophageal squamous cell carcinoma patients

et al. 2016

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Epithelial-mesenchymal transition (EMT) plays a key role in tumor metastasis, but the significance of EMT phenotype to the prognosis of esophageal squamous cell carcinoma (ESCC) patients remains unclear. We used immunohistochemistry to examine the expression of the EMT-related proteins E-cadherin, N-cadherin and vimentin in samples of T3N1-3M0 ESCC from 155 primary tumors (PTs) with paired metastatic lymph nodes (MLNs) and 58 PTs without paired MLNs. Based on the expression pattern of the EMT markers, PTs and MLNs were classified as EMT wild, hybrid, null or complete type. The hybrid (42.7%) and complete (39.4%) types predominated among PTs, whereas the wild (34.2%) and hybrid (52.9%) types predominated among MLNs, and EMT phenotypes differed between the paired PTs and MLNs (P < 0.001). Univariate analysis revealed that, for PTs, the EMT phenotype was associated with N-stage (P = 0.039) but not patient survival, and that patients with complete or hybrid type MLNs had better overall survival (OS, P = 0.001) and disease-free survival (DFS, P = 0.005) than patients with null and wild type MLNs, especially those with N1-stage disease (P = 0.017 for OS, and P = 0.017 for DFS, respectively). Multivariate analysis revealed that wild and null type MLNs as well as older age and N2-3 stage were independent predictors of OS and DFS (P < 0.05). Thus MLNs exhibit EMT phenotypes that are distinct from those of their PT and may serve as a novel independent prognostic indicator in ESCC.

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Section: Discussionsupporting

confidence: 85%

The epithelial-mesenchymal transition phenotype of metastatic lymph nodes impacts the prognosis of esophageal squamous cell carcinoma patients

et al. 2016

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“…In gastric cancers, vimentin expression was more associated with the invasive phenotype of gastric carcinoma and was suggested to play an important role in the metastasis of gastric carcinomas and serve as a prognostic marker in the detection of gastric cancers (81, 82). Analysis of esophageal squamous cell carcinoma samples from patients showed that vimentin expressing cells exhibit significantly higher incidence of lymph node metastasis (83). In HCC, vimentin expression was mainly associated with the metastatic HCC (84); however, in small HCC's, vimentin was detectable in the serum samples (66).…”

Section: Vimentin In Cancermentioning

confidence: 99%

“…Although its expression is more emphasized in the EMT process, it is equally possible that tumorigenic events including tumor cell migration and invasion are a consequence of vimentin over-expression in these cells, as most of the studies indicated a positive association of invasive phenotype to vimentin over-expression and showed a decrease in these characteristics upon knock-down of vimentin in vitro . Furthermore, vimentin's over-expression during metastasis (78, 83, 84, 101, 104) attracts much attention to further elucidate its role as a metastasis-promoter. However, these events are a result of fine tuning occurring in the cancer cells and vimentin might be acting as a scaffolding protein during signal transduction and promoting tumorigenic events in association with other tumor promoting oncogenes.…”

Section: Vimentin In Cancermentioning

confidence: 99%

Vimentin in cancer and its potential as a molecular target for cancer therapy

Satelli

2011

Cell. Mol. Life Sci.

1,288

1,052

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Vimentin, a major constituent of the intermediate filament (IF) family of proteins, is ubiquitously expressed in normal mesenchymal cells and is known to maintain cellular integrity and provide resistance against stress. Increased vimentin expression has been reported in various epithelial cancers including prostate cancer, gastrointestinal tumors, CNS tumors, breast cancer, malignant melanoma, lung cancer and other types of cancers. Vimentin's over-expression in cancer correlates well with increased tumor growth, invasion and poor prognosis; however, the role of vimentin in cancer progression remains obscure. In the recent years, vimentin has gained much importance as a marker for epithelial-mesenchymal transition (EMT). Although EMT is associated with a number of tumorigenic events, the role of vimentin in the underlying events mediating these processes remains unknown. Though majority of the literature findings indicate a future significance of vimentin as a biomarker for different cancers with clinical relevance, more research in to the molecular aspects will be crucial to particularly evaluate the function of vimentin in the process of tumorigenesis. By virtue of its over-expression in a large number of cancers and its role in mediating various tumorigenic events, vimentin serves as an attractive target for cancer therapy. Further, research directed toward elucidating the role of vimentin in various signaling pathways would open up new approaches for the development of promising therapeutic agents. This review summarizes the expression and functions of vimentin in cancers and also suggests some directions toward future cancer therapy utilizing vimentin as a potential target.

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“…It has been identified as a hallmark of metastasis in multiple tumors, connecting to plenty of transcriptional factors [36], [37], [38], [39]. miRNAs are also components of the cellular signaling circuitry that regulates the EMT program [40].…”

Section: Introductionmentioning

confidence: 99%

Identification of miRs-143 and -145 that Is Associated with Bone Metastasis of Prostate Cancer and Involved in the Regulation of EMT

Peng

Guo

Tiejian³

et al. 2011

PLoS ONE

205

185

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The principal problem arising from prostate cancer (PCa) is its propensity to metastasize to bone. MicroRNAs (miRNAs) play a crucial role in many tumor metastases. The importance of miRNAs in bone metastasis of PCa has not been elucidated to date. We investigated whether the expression of certain miRNAs was associated with bone metastasis of PCa. We examined the miRNA expression profiles of 6 primary and 7 bone metastatic PCa samples by miRNA microarray analysis. The expression of 5 miRNAs significantly decreased in bone metastasis compared with primary PCa, including miRs-508-5p, -145, -143, -33a and -100. We further examined other samples of 16 primary PCa and 13 bone metastases using real-time PCR analysis. The expressions of miRs-143 and -145 were verified to down-regulate significantly in metastasis samples. By investigating relationship of the levels of miRs-143 and -145 with clinicopathological features of PCa patients, we found down-regulations of miRs-143 and -145 were negatively correlated to bone metastasis, the Gleason score and level of free PSA in primary PCa. Over-expression miR-143 and -145 by retrovirus transfection reduced the ability of migration and invasion in vitro, and tumor development and bone invasion in vivo of PC-3 cells, a human PCa cell line originated from a bone metastatic PCa specimen. Their upregulation also increased E-cadherin expression and reduced fibronectin expression of PC-3 cells which revealed a less invasive morphologic phenotype. These findings indicate that miRs-143 and -145 are associated with bone metastasis of PCa and suggest that they may play important roles in the bone metastasis and be involved in the regulation of EMT Both of them may also be clinically used as novel biomarkers in discriminating different stages of human PCa and predicting bone metastasis.

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Vimentin expression of esophageal squamous cell carcinoma and its aggressive potential for lymph node metastasis

Cited by 56 publications

References 33 publications

The epithelial-mesenchymal transition phenotype of metastatic lymph nodes impacts the prognosis of esophageal squamous cell carcinoma patients

The epithelial-mesenchymal transition phenotype of metastatic lymph nodes impacts the prognosis of esophageal squamous cell carcinoma patients

Vimentin in cancer and its potential as a molecular target for cancer therapy

Identification of miRs-143 and -145 that Is Associated with Bone Metastasis of Prostate Cancer and Involved in the Regulation of EMT

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