Vinculin Binding Angle in Podosomes Revealed by High Resolution Microscopy

Walde, Marie; Monypenny, James; Heintzmann, Rainer; Jones, Gareth E.; Cox, Susan

doi:10.1371/journal.pone.0088251

Cited by 25 publications

(22 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…1C). We also visualised the typical polygonal arrangement of vinculin around F-actin and cortactin cores (Walde et al, 2014), and F-actin interconnecting cables by stimulated emission depletion (STED) microscopy (Labernadie et al, 2010;Luxenburg et al, 2007;van den Dries et al, 2013) (Fig. 1D).…”

Section: Hmvecs Spontaneously Assemble Podosomes and Vegf-a Stimulatementioning

confidence: 99%

VEGF-A stimulates podosome-mediated collagen-IV proteolysis in microvascular endothelial cells

Daubon

Spuul

Alonso

et al. 2016

Journal of Cell Science

View full text Add to dashboard Cite

Podosomes are dynamic cell-matrix contact structures that combine several key abilities, including adhesion, matrix degradation and mechanosensing. These actin-based cytoskeletal structures are well known in monocytic cells, but much less is known about those formed in other lineages. In this study, we characterize podosomes in capillary-derived microvascular endothelial cells. We identify two types of podosomes: constitutive podosomes that form in the absence of specific stimulation and induced podosomes that arise in response to the angiogenic factor VEGF-A. Constitutive and VEGF-A-induced podosomes share similar components but exhibit marked differences in terms of gelatinolytic activity. We also show that extracellular matrix proteins are key determinants of the VEGF-A response: laminin and collagen-IV, but neither collagen-I nor fibronectin are conducive for podosome induction. Moreover, only collagen-IV elicits the formation of proteolytically active podosomes through a mechanism involving increased Src phosphorylation, p190RhoGAP-B relocalisation and MT1-MMP cell surface exposure at podosome sites. We hypothesise that by promoting podosome formation, VEGF-A enables endothelial cells to overcome the basement membrane barrier for sprouting away from the existing vasculature.

show abstract

Section: Hmvecs Spontaneously Assemble Podosomes and Vegf-a Stimulatementioning

confidence: 99%

VEGF-A stimulates podosome-mediated collagen-IV proteolysis in microvascular endothelial cells

Daubon

Spuul

Alonso

et al. 2016

Journal of Cell Science

View full text Add to dashboard Cite

show abstract

“…Our previous study using replica TEM showed that these podosomal filaments consist of actin filaments decorated with myosin‐subfragment 1 (Akisaka & Yoshida, ). More recently, a new generation of super microscopies (Cox et al, ; van den Dries et al, a, b: Labernadie et al, ; Walde, Monypenny, Heintzmann, Jones, & Cox, ) has led to the current core‐ring model for podosome structures. The most fundamental advantage of these super‐microscopy techniques over EM is that living cells can be imaged.…”

Section: Introductionmentioning

confidence: 99%

Scattered podosomes and podosomes associated with the sealing zone architecture in cultured osteoclasts revealed by cell shearing, quick freezing, and platinum‐replica electron microscopy

Akisaka

Yoshida

2019

Cytoskeleton

View full text Add to dashboard Cite

Osteoclasts (OCs) can adhere to a variety of substrate surfaces by highly dynamic actin‐based cytoskeletal structures termed podosomes. This tight attachment is established by a sealing zone (SZ), which is made of interconnected individual podosomes. Compared with scattered podosomes in various cell types, the architecture of the SZ is still unclear. Especially, ultrastructural studies on the details of the cytoskeletal structure of an OC have been challenging, because the high density of filaments in their podosomes obscure visualization of individual filaments. Therefore, to study this organization in more exact detail, we employed shearing open combined with replica electron microscopy. The present study provides several new details of the podosome and SZ structure, which were previously unrecognized: (a) the SZ consists of recognizable podosomes with a dense actin network of interpodosomal regions characterized by multiple layers of crossing, branching and anastomosing actin filament networks; (b) the Arp2/3 complex is distributed throughout the actin network of podosomes and SZ, indicating that actin polymerization is concentrated at these regions; (c) a close spatial relationship between the podosome and the dorsal membrane; and (d) a network of membranous organelles in close proximity to the podosomes in the SZ. Taken together, the present study reveals that a more complicated interpodosomal actin network among neighboring individual podosomes, which is more complicated than previously thought, appears to form the SZ. Indeed, individual podosomes are not an isolated structural unit from other organelles; and, in turn, their dynamism might affect the surrounding interpodosomal cytoskeletons, membranous organelles, and plasma membrane.

show abstract

“…1). However, use of super resolution microscopy showed that the apparently continuous ring is an artifact of fluorescence microscopy, and that plaque proteins form several discrete clusters around the core structure [31][32][33].…”

Section: Introductionmentioning

confidence: 99%

Tools of the trade: podosomes as multipurpose organelles of monocytic cells

Linder

Wiesner

2014

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

Podosomes are adhesion and invasion structures that are particularly prominent in cells of the monocytic lineage such as macrophages, dendritic cells, and osteoclasts. They are multifunctional organelles that combine several key abilities required for cell migration and invasion. The podosome repertoire includes well-established functions such as cell-substrate adhesion, and extracellular matrix degradation, recently discovered abilities such as rigidity and topology sensing as well as antigen sampling, and also more speculative functions such as cell protrusion stabilization and transmigration. Collectively, podosomes not only enable dynamic interactions of cells with their surroundings, they also gather information about the pericellular environment, and are actively involved in its reshaping. This review presents an overview of the current knowledge on podosome composition, architecture, and regulation. We focus in particular on the growing list of podosome functions and discuss the specific properties of podosomes in macrophages, dendritic cells, and osteoclasts. Moreover, this article highlights podosome-related intracellular transport processes, the formation of podosomes in 3D environments as well as potentially podosome-associated diseases involving monocytic cells.

show abstract

Vinculin Binding Angle in Podosomes Revealed by High Resolution Microscopy

Cited by 25 publications

References 34 publications

VEGF-A stimulates podosome-mediated collagen-IV proteolysis in microvascular endothelial cells

VEGF-A stimulates podosome-mediated collagen-IV proteolysis in microvascular endothelial cells

Scattered podosomes and podosomes associated with the sealing zone architecture in cultured osteoclasts revealed by cell shearing, quick freezing, and platinum‐replica electron microscopy

Tools of the trade: podosomes as multipurpose organelles of monocytic cells

Contact Info

Product

Resources

About