The world’s changing climate is placing great pressure on the resources for sustainable viticulture. With this, it has become necessary to investigate grape varieties that are well adapted to hot climates. The aims of this study were to (1) assess the response of Xynisteri to different irrigation regimes, and (2) compare the performance of Xynisteri, Maratheftiko, Shiraz and Sauvignon Blanc grown in pots with different irrigation regimes. Trial one was established in a commercial Xynisteri vineyard in Cyprus under three different irrigation regimes - full, 50% and no irrigation in 2019. Trial two compared three irrigation regimes - full, 50% and 25% in a potted trial of Xynisteri and Sauvignon Blanc conducted in Cyprus in 2019. Trial three was a potted trial of Xynisteri, Sauvignon Blanc, Maratheftiko and Shiraz with the same three irrigation regimes conducted in Australia in 2020/21. Vine performance and physiology measurements were taken in both trials. Fruit composition analysis, yield (field trial only), shoot, trunk and root mass measurements were performed at the end of the season. Few differences between measures were found between irrigation regimes in the field trial. Fruit composition analysis revealed fructose to be lowest in the full irrigation group compared to deficit and no irrigation treatments. The potted trial in 2019 demonstrated that for all three irrigation regimes, Xynisteri had higher stem water potential, stomatal conductance and chlorophyll content than Sauvignon Blanc. Xynisteri produced greater end of season root, shoot and leaf mass than Sauvignon Blanc under all irrigation regimes. In 2020/21, Xynisteri had greater end of season root, shoot and leaf mass than Maratheftiko and Sauvignon Blanc with Shiraz the lowest. Few significant differences in stem water potential were observed in the early stages of the trial. However, toward the end of the trial and with reduced irrigation, Xynisteri and Maratheftiko had higher stem water potential than Shiraz and Sauvignon Blanc. Xynisteri had higher stomatal conductance and chlorophyll content than Maratheftiko and both were higher than Sauvignon Blanc and Shiraz. These results indicate that Xynisteri in particular may possess better cultivar specific growth traits than Shiraz and Sauvignon Blanc when grown under the same environmental conditions and in turn may be a more appropriate choice in areas where water is limited.