Objectives: Nursing staff is at potential danger of getting HCV infection. Objectiveof present study is to determine the prevalence and associated risk factors of hepatitis C amongnurses in Lahore, Pakistan. It also aimed to estimate the HCV genotypes and find out anyrelationship between their working area and the incidence of HCV infection. Setting: Variouspublic and private hospitals of Lahore. Period: January 2013 to December 2013. Subjectsand Method: This study comprised 186 Nurses. All the samples were processed for Anti-HCVantibody detection through ELISA by using third generation ELISA Kit. Genotyping was alsoperformed on all positive samples. In this study the data were analyzed using SPSS version 16.A P-value < 0.05 was considered to be significant. Results: A total of 186 nurses were enrolledand screened for Anti HCV in the study. The mean age of reactive and non reactive nurses was22.33±1.15 and 23.66±1.97 years, respectively. Working experience in surgical wards, habitsfor going to beauty salon as well as the purpose for which nurses visited beauty salon wassignificantly associated with HCV status. i.e. p-value<0.05. Out of 9 reactive nurses, 8 patientnurses had 3a viral genotype while 01 patient had un-typeable viral genotype. Conclusion: Inour study nine nurses have HCV infection and are at danger of the disease.Theworking areaespecially surgical wards is a source of infection of HCV.