Viral Etiology Acute Intestinal Infections Molecular Monitoring in Children’s Hospital

Sergeeva, A. V.; Poslova, L.Yu.; Ковалишена, О. В.; Благонравова, А С; Епифанова, Н. В.; Сашина, Т. А.; Морозова, О. В.; Новикова, Н. А.

doi:10.15789/2220-7619-2015-3-243-252

Cited by 3 publications

(4 citation statements)

References 13 publications

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“…Достаточно широкое внедрение молекулярно-биологических лабораторных методов диагностики внесло существенный вклад в представление об эпидемиологии острых диарей (ОД) и их современной структуре [9,12,22]. В настоящее время в Российской Федерации, как и в других странах, лидерами среди вирусных возбудителей острых диарей, наиболее значимыми по частоте возникновения как спорадической, так и вспышечной заболеваемости, являются ротавирусы и норовирусы [7,8,18,13].…”

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Characteristics of acute diarrhea adult cases with samples positive for Norwalk virus

Кожухова

Gorbova²

2019

Russian Journal of Infection and Immunity

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Remaining unmanageable,Norwalkvirus infection is clearly tended to be recorded at higher rate, including adult patients. In many cases, clinical picture of adult acute diarrhea in patients positive for Norwalk virus in clinical samples vs. pathogenetically-caused norovirus infection differs, thereby justifying comparison of clinical and laboratory data. A cohort retrospective study with 146 hospitalized adult patients suffering from acute moderate diarrhea positive for fecalNorwalkvirus was performed. Along with standard laboratory tests (culturing, serologic for detecting 4-fold increase in titer between paired serum samples, ELISA), detection of diarrhea-linked agents included PCR kit Amplisens® AII-bacto-screen-FL Lab (Interlabservice). The data obtained demonstrated that in adult patients with acute diarrhea 54.1% of cases were positive forNorwalkvirus as well as for other enteric pathogens, including bacteria found in 36.3% of cases. Moreover, clinical samples of patients with acute diarrhea hospitalized at least on day 4 vs. day 3 after the onset were significantly more often (by 1.5-fold) positive for association between Norwalk virus and other acute enteric infection agents mainly due to astrovirus (р = 0.03; PCR data) and Shigella spp. (culture-based and serologic methods; р = 0.03). In addition,Norwalkvirus was associated with rotavirus, but not other enteric pathogens, at 2.1-fold higher rate in clinical samples from patients treated vs. untreated with antimicrobials before hospitalization. Finally, clinical samples positive for Norwalk virus from patients with vs. without developed colitis syndrome were at higher risk of developing virus-bacterial enteric infection detecting Salmonella spp. at 7.6and 3-fold higher rate verified by culture-based and PCR assay, respectively. Importantly, patients with vs. without hemorrhagic colitis Salmonella spp. verified primarily a culture-based method was detected by 11-fold more frequently (p = 0.01).

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Section: Introductionunclassified

Characteristics of acute diarrhea adult cases with samples positive for Norwalk virus

Кожухова

Gorbova²

2019

Russian Journal of Infection and Immunity

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“…At the first stage of the study, it was found that enteric viruses were detected in 43.8% of patients with the syndrome of gastrointestinal dysfunction unrelated to the underlying disease (95% CI 40.1-47.5) [4]. Among the persons infected with enteric viruses, mono-infection was diagnosed in 72% of patients, mixed infection in 28% (p=0.007).…”

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confidence: 97%

“…Studying different aspects of the problem of acute enteric infections (AEI) of viral etiology is urgent today due to their wide spread, polyetiology, variety of transmission ways and factors, marked seasonality, the continuing upward trend in incidence often acquiring group character and occurring as outbreaks, particularly among pediatric population [1][2][3][4][5][6]. The outbreaks of viral AEI can be also registered in various healthcare institutions specializing in both infectious and noninfectious diseases [1,3,7,8].…”

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Developing the Molecular Genetic Component of Microbiological Monitoring of Nosocomial Acute Enteric Viral Infections

Shkarin¹,

Sergeeva²,

Poslova³

et al. 2017

Sovrem Tehnol Med

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The aim of the investigation was improving the effectiveness of microbiological monitoring through optimization and development of the molecular genetic component based on comprehensive long-term assessment of hospital-acquired acute enteric infections of viral etiology.Materials and Methods. In the framework of epidemiological surveillance of nosocomial viral infections, syndrome-based diagnosis of acute enteric infection cases was implemented in a children's hospital. In fact, the patients with the signs of gastrointestinal dysfunctions unrelated to the underlying disease were identified and examined. DNAs (RNAs) of acute enteric infections of viral etiology were detected and differentiated through PCR-based diagnosis. G[P]-typing of rotaviruses was performed by RT-PCR method using various primers. Genotyping of enteric viruses by sequencing method was performed determining the relevant nucleotide sequences of rotavirus, norovirus and astrovirus cDNA segments using genetic analyzer Beckman Coulter. Nucleotide sequences of cDNA segments were analyzed using BLAST programs to identify closely related strains and an online service for automatic genotyping of noroviruses. Alignment of nucleotide sequences and phylogenetic analysis were performed using MEGA software. Sequences of genome segments obtained in this study have been represented in the international GenBank database.Results. There has been developed the molecular genetic component of microbiological monitoring of acute enteric infections of viral etiology, which involves not only diagnosing the enteric pathogens by PCR, but also subsequent genotyping as well as phylogenetic analysis to determine genetic characteristics of pathogens.

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Characteristics of acute diarrhea in adult cases with <i>Campylobacter</i> spp. positive fecal samples

Kozhukhova,

Andreeva,

Dmitrieva

et al. 2023

Russian Journal of Infection and Immunity

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Recently, cases caused by Campylobacter spp. in Russia similar to other countries tended to clearly rise, partly due to the widely implemented biomolecular PCR-based laboratory analysis that substantially facilitated Campylobacter spp. detection in patients samples. The problem of being infected by Campylobacter spp. is so far worth studying because of campilbacteriosis signs in a form of a variety of clinical patterns including local and general forms, possible trigger role in forming the consequent chronic digestive tract pathology as well as general systemic pathology. Development of colitis or heamocolitis as the pattern part of acute diarrhea caused by Campylobacter spp. is usually considered as associated with the disease burden. So far as adult patients have acute diarrhea with positive Campylobacter spp. samples it might be of interest to compare and analyze their clinical and laboratory data to find some possible associations as particular features of different disease patterns. Objective to analyze clinical features and laboratory data of hospitalized AD adult cases with feces samples positive for Campylobacter spp. When carrying out the retrospective cohort study the analyzed were clinical and laboratory data of 111 hospitalized adult patients suffering from moderate acute diarrhea with clinical samples (feces) positive for Campylobacter spp. To verify acute infectious diarrhea all patients went through complex specific laboratory examination that included PCR based methods (feces) with use of Amplisense AD screen-FL in addition to routine standard culture (feces), serologic (specific antibody detection) and ELISA laboratory methods. The analysis results have shown that more than 1/2 part of patients developed colitis syndrome with blood in feces in practically every forth case. In patients (predominantly males) without chronic digestive tract problems relative risks to develop colitis and haemocolitis are respectively 1.6 and 2.6 times higher given they are hospitalized on day 4 after the onset and later versus those hospitalized earlier.

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Viral Etiology Acute Intestinal Infections Molecular Monitoring in Children’s Hospital

Cited by 3 publications

References 13 publications

Characteristics of acute diarrhea adult cases with samples positive for Norwalk virus

Characteristics of acute diarrhea adult cases with samples positive for Norwalk virus

Developing the Molecular Genetic Component of Microbiological Monitoring of Nosocomial Acute Enteric Viral Infections

Characteristics of acute diarrhea in adult cases with <i>Campylobacter</i> spp. positive fecal samples

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