Viral etiology of Acute Respiratory Infections in Hospitalized Children in Novosibirsk City, Russia (2013 – 2017)

Kurskaya, Olga; Ryabichenko, T. I.; Leonova, N.V.; Shi, Weifeng; Bi, Haibo; Sharshov, Kirill; Kazachkova, E. A.; Sobolev, Ivan; Prokopyeva, Elena A.; Карцева, Т. В.; Alekseev, A. A.; Шестопалов, А. М.

doi:10.1101/353037

Cited by 21 publications

(47 citation statements)

References 43 publications

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“…In our study, gender had no influence on the detection rate of respiratory viruses, which was consistent with other reports . Many studies documented that respiratory viral infection was characterized by seasonal distributions, with their peaks lasting from winter to early spring .…”

Section: Discussionsupporting

confidence: 92%

See 1 more Smart Citation

Detection and clinical characteristics analysis of respiratory viruses in hospitalized children with acute respiratory tract infections by a GeXP‐based multiplex‐PCR assay

Huang

Chen

Zhang

et al. 2019

Clinical Laboratory Analysis

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Background: The information regarding viral epidemiology and clinical characteristics in hospitalized children with acute respiratory tract infection (ARTI) in central Fujian is limited. In this study, we aimed at analyzing the viral epidemiology and clinical characteristics of ARTI in hospitalized children admitted to The First Affiliated Hospital of Fujian Medical University. Methods: Cohort of 386 hospitalized children (31 days to 15 years) diagnosed with ARTI admitted to the Department of Pediatrics from January 1, 2018, to December 31, 2018, was enrolled in this study. Nasopharyngeal swab or sputum samples on the day of hospitalization were tested for 11 viruses via a GeXP-based multiplex-PCR assay. The viral profiles and clinical characteristics were analyzed. Results: The overall positive rate of the samples was 43.26% (167/386). Among the 167 positive samples, 134 (80.24%, 134/167) had a single virus and 33 (19.76%, 33/167) had multiple viruses. There was a significant difference in the frequency of single vs mixed infections among positive samples (80.24% vs 19.76%; χ 2 = 122.168, P = .000)as well as among the total examined samples (34.72% vs 8.55%; χ 2 = 77.945, P = .000).Human rhinovirus was the most prevalent virus (17.36%, 67/386), followed by influenza A (5.96%, 23/386) and human adenovirus (5.70%, 22/386). There was no significant difference in the etiological distribution of viral pathogens between males and females (χ 2 = 0.480, P = .489). Viral infections were more likely to occur in the winter-spring months than in the summer-autumn months (52.51% vs 33.53%, χ 2 = 13.830, P = .000). Conclusions:The GeXP-based multiplex PCR is an accurate and high-throughput assay allows us to quickly detect multiple respiratory viruses simultaneously in pediatric patients. Our study provides information on the viral profiles and clinical characteristics in hospitalized children with ARTI, which would help better effective prevention strategies. K E Y W O R D S acute respiratory tract infection, children, clinical characteristics, multiplex PCR How to cite this article: Huang H, Chen S, Zhang X, Hong L, Zeng Y, Wu B. Detection and clinical characteristics analysis of respiratory viruses in hospitalized children with acute respiratory tract infections by a GeXP-based multiplex-PCR assay.

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Section: Discussionsupporting

confidence: 92%

“…It is well known that ARTI prevalence in children may vary in different geographic regions and different seasons . However, information regarding viral ARTI in pediatric hospitalized children in Fuzhou city (central Fujian) is still limited.…”

Section: Introductionmentioning

confidence: 99%

Detection and clinical characteristics analysis of respiratory viruses in hospitalized children with acute respiratory tract infections by a GeXP‐based multiplex‐PCR assay

Huang

Chen

Zhang

et al. 2019

Clinical Laboratory Analysis

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“…Healthcare attendees were studied in order to increase the likelihood of detecting exposure of humans to the pathogens since parents of children with febrile illness or influenza-like illness are more likely to seek healthcare attention. The finding is similar to the prevalence of 22.1% influenza infection reported in Russian children between 2013 and 2017 [24]. A study conducted in Germany (2008-2010) showed a higher IgG seroprevalence of 87.6% to influenza virus using ELISA technique [25].…”

Section: Discussionsupporting

confidence: 85%

Influenza, Malaria parasitaemia and Typhoid fever Coinfection in Children: Seroepidemiologic Investigation in Four Healthcare Centres in Lagos, Nigeria

Anjorin

Babalola

Iyiade

2019

Preprint

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Background : According to WHO, out of about one billion individuals usually infected with influenza virus, children from developing countries account for 99% of deaths due to related infections. There is dearth of clinical-epidemiological information on the trio of influenza, malaria, and typhoid fever co-infection in Nigeria. Similarity of their clinical symptoms coupled with lack of vaccine and all year-round circulation in sub-Saharan Africa cause serious paediatric morbidity and mortality. This study therefore investigated influenza, malaria and typhoid fever coinfection among children in Lagos, Nigeria. Methods : A sero-epidemiologic hospital-based study was designed. Blood from 364 children tested by RDT for malaria HRP-II/pLDH (Accessbio or Medicon BioCheck, USA) and typhoid (CTKBiotech, USA or Omega, UK) were screened by ELISA (Demeditec, Germany) for influenza virus specific IgM antibody. Descriptive statistics was used and p-values were determined with Chi-square. Results : Demographic data showed median age of 3 (mean 3.8, mode 2, range 0-14) years. Out of the 364 samples tested, 76/364 (20.9%) were seropositive for influenza A virus. Of the 76 seropositive patients, 47/76 (61.8%) had malaria parasitaemia, 42/76 (55.3%) had typhoid, and 21/76 (27.6%) were co-infected with malaria parasitaemia and typhoid fever. Furthermore, 2/76 (2.6%) children having underlying health condition of sickle cell anaemia recorded influenza seropositivity. Ojo primary healthcare centre had the highest seroprevalence of 48.7% (37/76), age group 1-4 years recorded the highest seroprevalence of 55.3% (42/76), the highest serologic evidence of 61.8% (47/76) was detected among male children, while fever 27.6% (21/76) was the most common of all the clinical symptoms recorded. Although, the month of March had the highest seroprevalence of 20/76 (26.3%), seropositivity was recorded in all other months considered for this study. Conclusions : We hereby report the first paediatric co-infection of influenza, malaria and typhoid fever with a percentage seroprevalence of 27.6% among all age group round the months. Low co-infection was however recorded in children having sickle cell anaemia. Annual vaccination is strongly recommended for children of all ages in order to prevent co-infection of influenza with other deadly diseases.

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“…Respiratory virus is the main cause of infection [2]. Nasopharynx swabs or oropharynx swabs are commonly used as the pathogen detection samples of ARIs [3,5,6]. In this study, the detection of four respiratory viruses (ADV, FLUA, FLUB, and RSV) in oropharyngeal swabs of 103210 children with ARIs was analyzed retrospectively.…”

Section: Discussionmentioning

confidence: 99%

“…Acute respiratory infections (ARIs) are common respiratory diseases in childhood. ARIs are the main cause of morbidity, hospitalization and death of children, and also cause economic loss of family and society [1][2][3]. Viruses are the main infectious agents of ARIs [4], among which respiratory syncytial virus (RSV), rhinovirus, human metapneumovirus, parain uenza virus, human enterovirus, in uenza virus (Flu), human coronavirus, adenovirus (ADV) and human Boca virus account for about 70% of ARIs viral infection sources [2].…”

Section: Introductionmentioning

confidence: 99%

Epidemiological characteristics of four common respiratory viral infections in children

Zhu

Zhang

et al. 2020

Preprint

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Abstract Background: Viruses are the main infectious agents of ARIs in children. We aim to describe the epidemiological characteristics of viral pathogens of acute respiratory tract infections in outpatient children. Methods: From April 2018 to March 2019, the results of viral detection in oral pharyngeal swabs in 103 210 children with acute respiratory tract infection in the outpatient department of the Children’s Hospital, Zhejiang University School of Medicine were retrospectively analyzed. Viral antigens, including adenovirus (ADV), influenza A (FLUA), influenza B (FLUB) and respiratory syncytial virus (RSV) were detected by colloidal gold method. Results: At least one virus was detected in 38 355 cases, and the total positive rate was 37.2%. A total of 1910 cases of mixed infection with two or more viruses were detected, and the positive rate of multiple infection was 1.9%. ADV positive rate was the highest in 3-6-year-old group (18.7%), FLUA positive rate was the highest in >6-year-old group (21.6%), FLUB positive rate was the highest in >6-year-old group (6.6%) and RSV positive rate was the highest in <1-year-old group (10.6%). There was significant difference in the positive rate of viral infection among different age groups, χ2 = 1280.7, P < 0.001. The positive rate of viral infection was the highest in winter (47.1%). ADV infection rate was highest in spring (18.2%). The positive rates of FLUA and FLUB were the highest in winter (28.8% and 3.6%, respectively). The positive rate of RSV was the highest in autumn (17.4%). The positive rate of viral infection in different seasons had significant difference, χ2 = 6459.1, P < 0.001. Conclusions: Viral infection rates in children are different in different ages and seasons. ADV positive rate is the highest in preschool age, RSV positive rate is the highest in infants, and FLU positive rate increases with age. The total positive rate of viral infection in different seasons was the highest in winter. The positive rate of FLU was the highest in winter.

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Viral etiology of Acute Respiratory Infections in Hospitalized Children in Novosibirsk City, Russia (2013 – 2017)

Cited by 21 publications

References 43 publications

Detection and clinical characteristics analysis of respiratory viruses in hospitalized children with acute respiratory tract infections by a GeXP‐based multiplex‐PCR assay

Detection and clinical characteristics analysis of respiratory viruses in hospitalized children with acute respiratory tract infections by a GeXP‐based multiplex‐PCR assay

Influenza, Malaria parasitaemia and Typhoid fever Coinfection in Children: Seroepidemiologic Investigation in Four Healthcare Centres in Lagos, Nigeria

Epidemiological characteristics of four common respiratory viral infections in children

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