Viral Genome Structure

Symons, Robert H.

doi:10.1007/978-1-4684-4937-2_3

Cited by 20 publications

(10 citation statements)

References 69 publications

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“…For example, there is a match of 12 nucleotides in maize chlorotic mottle virus R N A (Lommel et al, 1991), up to 10 in tobacco rattle virus R N A (TRV; Cornelissen et al, 1986) and up to 11 in alfalfa mosaic virus R N A (Symons, 1985). As suggested by Cornelissen et al (1986) for T R V R N A , this sequence similarity at the 5' ends may reflect a replicase recognition signal in the corresponding minus-strand RNA.…”

Section: Discussionmentioning

confidence: 96%

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The location of the 5' end of the potato leafroll luteovirus subgenomic coat protein mRNA

Miller¹,

Mayo²

1991

Journal of General Virology

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Section: Discussionmentioning

confidence: 96%

“…RNA from particles of tobacco mosaic virus (6.4 kb; Goelet et al, 1982) and brome mosaic virus (BMV, 3.2 kb, 2.9 kb, 2.1 kb and 0.9 kb; Symons, 1985) was used as an Mr marker.…”

Section: Methodsmentioning

confidence: 99%

The location of the 5' end of the potato leafroll luteovirus subgenomic coat protein mRNA

Miller¹,

Mayo²

1991

Journal of General Virology

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“…Protein extracted from infected protoplasts and from infected C. quinoa tissue contained a 120K polypeptide (Symons, 1985), numbers to the right indicate the proteins present in infected protoplasts (lanes 2) but not in mock-inoculated protoplasts (lanes 1). The arrows indicate the tops of the gels.…”

Section: Serological Detection Of the 120k Polypeptidementioning

confidence: 99%

Virus-specific proteins in cells infected with tomato black ring nepovirus: evidence for proteolytic processing in vivo

Demangeat

Hemmer²,

Reinbolt³

et al. 1992

Journal of General Virology

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The synthesis of proteins encoded by the RNA of tomato black ring virus (TBRV) in vivo was studied in protoplasts by direct labelling with [35S]methionine, and in protoplasts and plants by immunoblotting experiments with specific antisera. Comparison of the proteins synthesized in infected and mock-inoculated protoplasts suggested that proteins of Mr 120K, 90K, 80K, 57K and 46K were virus-specific. The proteins derived from the RNA-l-encoded polyprotein detected by immunoblotting were a stable 120K protein and, only in protoplasts, small amounts of a 90K protein which contains the C-terminal part of the 120K protein and the polymerase domain. The results suggest that the polymerase and the adjacent protease function in vivo largely or solely when combined in a 120K protein.The proteins derived from the RNA-2-encoded polyprotein detected by immunoblotting were 59K and 57K proteins, which reacted with antiserum to TBRV particles, and a 46K protein. In extracts of infected Nicotiana clevelandii and Chenopodium quinoa made soon after inoculation, the 59K protein was more abundant than the 57K protein; later samples contained similar quantities of each protein. The 57K protein comigrated with protein extracted from virus particles. The results of amino acid sequencing suggested that the 57K protein is derived from the 59K protein by the loss of nine C-terminal amino acids. Antiserum to a peptide adjacent to the 57K protein in the 150K polyprotein detected a 46K protein in protoplasts and plant tissue. The results support the processing scheme for TBRV polyproteins proposed after analysis of the products of in vitro translation.

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“…The coat protein gene in RNA 3 is not expressed directly but as a subgenomic, highly effective message -RNA 4 -of approx. 0"9 kb (Symons, 1985). CiLRV and CVV both have coat proteins with an M r of 26000 which are serologically related to each other (Garnsey, 1975).…”

mentioning

confidence: 99%

The complete nucleotide sequence of RNA 3 of citrus leaf rugose and citrus variegation ilarviruses

Scott

Ge²

1995

Journal of General Virology

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Complete sequence data for the RNA 3 of both citrus leaf rugose (CiLRV) and citrus variegation (CVV) ilarviruses have been determined. The RNAs are 2289 nt (CiLRV) and 2309 nt (CVV) in length and both contain the typical Bromoviridae arrangement of two open reading frames (ORFs) which, when translated, code for proteins that correspond to the M r 32 000 (32K) putative movement proteins (ORF 1) and the coat proteins (ORF 2) of the respective viruses. The 3' termini of both viruses can be folded to form a secondary structure similar to those reported for other ilarviruses. These are the first complete nucleotide sequences for RNA 3 of members of subgroup 2 of the ilarviruses. The two viruses share substantial homology in nucleic acid sequence, code for identically sized coat proteins and share high levels of identity in the translated products of both ORFs. Although related, these viruses differ sufficiently to be considered distinct. The RNA 3s of CiLRV and CVV appear to be distinct from those of other ilarviruses for which comparable sequence data are available and also from the closely related alfalfa mosaic virus.Citrus leaf rugose virus (CiLRV) and citrus variegation virus (CVV) are two serologically related but biologically distinct ilarviruses that infect citrus (Garnsey, 1975). Genomes of ilarviruses consist typically of three RNA species of approx. 3.4 kb (RNA 1), 2.9 kb (RNA 2) and 2"2 kb (RNA 3). While RNA 1 and RNA 2 are both monocistronic, RNA 3 is bicistronic with ORF 1 coding for a putative movement protein and ORF 2 coding for the viral coast protein. The coat protein gene in RNA 3 is not expressed directly but as a subgenomic, highly effective message -RNA 4 -of approx. 0"9 kb (Symons, 1985). CiLRV and CVV both have coat proteins with an M r of 26000 which are serologically related to each other (Garnsey, 1975). As groupings within the ilarviruses are based on serological relationships both are members of subgroup 2 which also includes elm mottle virus, Tulare apple mosaic virus and asparagus virus II (Francki et al., 1991).

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Viral Genome Structure

Cited by 20 publications

References 69 publications

The location of the 5' end of the potato leafroll luteovirus subgenomic coat protein mRNA

The location of the 5' end of the potato leafroll luteovirus subgenomic coat protein mRNA

Virus-specific proteins in cells infected with tomato black ring nepovirus: evidence for proteolytic processing in vivo

The complete nucleotide sequence of RNA 3 of citrus leaf rugose and citrus variegation ilarviruses

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