Viral load of human papillomavirus types 16/18/31/33/45 as a predictor of cervical intraepithelial neoplasia and cancer by age

Malagón, Talía; Louvanto, Karolina; Ramanakumar, Agnihotram V.; Koushik, Anita; Coutlée, François; Franco, Eduardo L.

doi:10.1016/j.ygyno.2019.09.010

Cited by 18 publications

(24 citation statements)

References 39 publications

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“…A higher viral load is also likely to be associated with immunological factors affecting VAIN progression, such as genetic susceptibility. Further studies are needed to verify these mechanisms (9). Furthermore, we discovered that, for women with cervical lesions, the viral load of HPV showed little difference whether accompanied with vaginal lesions or not.…”

Section: Discussionmentioning

confidence: 91%

“…These studies discovered that a group of α9 species including HPV16, 31, 33, 35, 52 and 58 were positively associated with the severity of CIN, and the optimal cutoffs of the log10-transformed viral loads of these specific HPV types might help identify high-grade squamous intraepithelial lesions (HSIL). Controversy surrounds the correlation between CIN and α7 species such as HPV18 (7)(8)(9). However, few studies have explored the correlation of HPV types, viral load and vaginal lesions, or the potential role of HPV viral load to serve as an indicator for VAIN.…”

Section: Introductionmentioning

confidence: 99%

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Types and viral load of human papillomavirus, and vaginal microbiota in vaginal intraepithelial neoplasia: a cross-sectional study

Zhou¹,

Zhou²,

Zhu³

et al. 2020

Ann Transl Med

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Background: Human papilloma virus (HPV) infection is an important risk factor for vaginal intraepithelial neoplasia (VAIN). Recent studies have suggested that the microbiome may play a potential role in cervicovaginal diseases. This study aimed to explore the characteristics of the types and viral load of HPV in VAIN, as well as the association between vaginal microbiota and VAIN.Methods: A total of 176 women, either with VAIN, or without VAIN but with HPV infection were enrolled in the study. Among them, 109 HPV positive cases were qualified for viral load assay. The vaginal microbiota of 122 HPV positive women, who were matched by severity of cervical lesions and menopause status, was determined by 16S ribosomal RNA (16S rRNA) sequencing. Results:The top 5 types of HPV-associated vaginal lesions were HPV16 (24.2%), HPV52 (24.2%), HPV53 (16.1%), HPV58 (14.5%) and HPV66 (14.5%). The viral load of HPV types 16, 52, and 58 appeared higher in separate vaginal lesions than in histopathologically normal cases (P=0.026, 0.002, and 0.013, respectively). The vaginal microbiota of HPV-positive patients with VAIN did not exhibit a large change in diversity. Vaginal microbiota of VAIN was characterized by an increased abundance of Atopobium, Gardnerella, Allobaculum and Clostridium, as well as decreased abundance of Finegoldia, Actinobaculum and Blautia. A higher level of Enterococcus and some specific Clostridium spp. might be associated with an elevated risk of VAIN2/3.Conclusions: A higher level of viral load of HPV16, 52, and 58 may indicate VAIN. The composition of vaginal microbiota changes during the progression of VAIN and specific bacteria such as Atopobium, Gardnerella, Allobaculum, Enterococcus and Clostridium, may help to promote its development.

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Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Types and viral load of human papillomavirus, and vaginal microbiota in vaginal intraepithelial neoplasia: a cross-sectional study

Zhou¹,

Zhou²,

Zhu³

et al. 2020

Ann Transl Med

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“…Cervical cancer is the most common gynecological cancer among women and the high-risk HPV genotypes play a major role in abnormal lesion development and cervical malignant neoplasms (Malagón et al, 2019;So et al, 2019). The presence of a high viral load in HPV-positive women indicates that the virus has not been entirely removed and will likely continue to replicate in the body cells for a long time.…”

Section: Discussionmentioning

confidence: 99%

Association between ТР53, MDM2 and NQO1 gene polymorphisms and viral load among women with human papillomavirus

Albosale

Машкина

2022

Vestn. VOGiS

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The risk of cervical cancer is caused by persistent human papillomavirus (HPV) infection. Cervical cancer is the most frequent cancer among women. Our purpose was to investigate the association between TP53 215C>G (Pro72Arg), MDM2 -410T>G, and NQO1 609C>T gene polymorphisms with a high HPV load and the influence of gene-gene interactions on prolonged HPV infection. Eighty-nine women with a high HPV viral load and 114 healthy women were involved in a case–control study. Genotyping for TP53 215C>G (Pro72Arg) and MDM2 -410T>G SNPs was carried out by allele-specific PCR and genotyping for NQO1 609C>T was performed by a TaqMan assay. Quantitative analysis of HPV DNA was performed by AmpliSens® HPV HCR screen-titer-FRT test system. Gene-gene interactions were analyzed using the multifactor dimensionality reduction (MDR) method. The study of separate SNPs of MDM2 -410T>G and NQO1 609C>T genes did not reveal any statistically significant difference in genotype and allele frequencies among women within the two groups. The frequency of the 215G (72Arg) allele and 215GG (72Arg/Arg) genotype of the TP53 gene was significantly higher in the case group than in the control group (OR = 1.74, 95 % CI = 1.10–2.73; p = 0.02 and OR = 1.97, 95 % CI = 1.13–3.46; p = 0.04, respectively). MDR analysis showed the significance of intergenic interactions of the three studied loci TP53 (rs1042522) – MDM2 (rs2279744) – NQO1 (rs1800566) for the formation of a high HPV load (OR = 3.05, 95 % CI = 1.73–5.46; p = 0.0001).

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“…2 ). The importance of the amount of HPV copies has been highlighted since their increase has been seen to be related to the appearance of cervical lesions (a dose–response relationship) 16 and a greater probability of STI transmission 32 . An increase in the amount of viral copies leads to an increase in the amount of infected cells and viral DNA integration, meaning that many cellular targets are required for successful HPV propagation 33 .…”

Section: Discussionmentioning

confidence: 99%

“…This is why parasite infection is considered a key risk factor regarding HPV persistence 12 – 14 . Understanding the factors involved in HPV persistence is thus relevant as it has been clearly demonstrated that the virus’ permanence in an organism is a key factor in cervical cancer (CC) development 15 , 16 .…”

Section: Introductionmentioning

confidence: 99%

Trichomonas vaginalis follow-up and persistence in Colombian women

Hernández-Buelvas

Camargo

Sánchez

et al. 2021

Sci Rep

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Trichomonas vaginalis (TV), the most common non-viral sexually-transmitted infection is considered a neglected infection and its epidemiology is not well known. This study determined TV-infection dynamics in a retrospective cohort of Colombian women and evaluated associations between risk factors and TV-outcome. TV was identified by PCR. Cox proportional risk models were used for evaluating the relationship between TV-outcome (infection, clearance and persistence) and risk factors (sexually-transmitted infections and sociodemographic characteristics). Two hundred and sixty-four women were included in the study; 26.1% had TV at the start of the study, 40.9% suffered at least one episode of infection and 13.0% suffered more than one episode of TV during the study. Women suffering HPV had a greater risk of TV-infection (aHR 1.59), high viral-load (> 102) for HPV-16 being related to a greater risk of persistent parasite infection; a high viral load (> 102) for HPV-18 and -33 was related to a lower probability of TV-clearance. Ethnicity (afrodescendent/indigenous people: aHR 5.11) and having had more than two sexual partners (aHR 1.94) were related to greater risk of infection, contrasting with women having a background of abortions and lower probability of having TV (aHR 0.50). Women aged 35- to 49-years-old (aHR 2.08), increased years of sexual activity (aHR 1.10), multiple sexual partners (aHR 8.86) and multiparous women (aHR 3.85) led to a greater probability of persistence. Women whose cervical findings worsened had a 9.99 greater probability of TV-persistence. TV distribution was high in the study population; its coexistence with HPV and other risk factors influenced parasite infection dynamics. The results suggested that routine TV detection should be considered regarding populations at risk of infection.

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Viral load of human papillomavirus types 16/18/31/33/45 as a predictor of cervical intraepithelial neoplasia and cancer by age

Cited by 18 publications

References 39 publications

Types and viral load of human papillomavirus, and vaginal microbiota in vaginal intraepithelial neoplasia: a cross-sectional study

Types and viral load of human papillomavirus, and vaginal microbiota in vaginal intraepithelial neoplasia: a cross-sectional study

Association between ТР53, MDM2 and NQO1 gene polymorphisms and viral load among women with human papillomavirus

Trichomonas vaginalis follow-up and persistence in Colombian women

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