Viral Polymorphism in Human Papillomavirus Types 33 and 35 and Persistent and Transient Infection in the Genital Tract of Women

Gagnon, Simon; Hankins, Catherine; Tremblay, Cécile; Forest, Pierre; Pourreaux, Karina; Coutlée, François

doi:10.1086/424854

Cited by 37 publications

(73 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, other prospective studies found the same molecular variant of HPV-16 in 100% of the cases over follow-up time (14). The same was observed in infections of HPV types 33, 35, and 52 (11,13).…”

Section: Nucleotide Variability and Risk Of Cervical Neoplasiasupporting

confidence: 57%

“…The association of specific variants of HPVs 33, 35, 52, and 58 with the persistence of the infection and with the severity of the neoplasia has also been observed (9,11,13). Taken together, these results indicate that, epidemiologically, the oncogenicity of molecular variants of HPVs 16, 18, and possibly other types, appears to vary not only geographically, but also with the ethnic origin of the populations under study.…”

Section: Nucleotide Variability and Risk Of Cervical Neoplasiamentioning

confidence: 53%

“…Today, papillomaviruses are a taxonomic family of their own, Papillomavirinae, and comparative nucleotide sequence analysis of these viruses has elucidated some features of their phylogenetic relationship (1). Concerning HPV intratypic nucleotide heterogeneity, the most extensive studies have been conducted on HPV-16 (3)(4)(5), followed by HPVs 18 and 45 (6), HPVs 6 and 11 (7), HPVs 5 and 8 (8), and more recently HPVs 58 (9,10), 31, 33, 35, and 52 (11)(12)(13).…”

Section: Papillomavirus Heterogeneitymentioning

confidence: 99%

“…The prototype of HPV-52 was detected in a biopsy sample from a woman living in the US. Other associations between race and infection by different molecular variants of HPVs 31, 33, and 35 have also been described (13). However, excluding the study by Hildesheim and collaborators (17), in all other studies, race classification was based on skin color which is not an ideal method to determine the degree of genetic relatedness among individuals.…”

Section: Nucleotide Variability and Risk Of Cervical Neoplasiamentioning

confidence: 99%

See 3 more Smart Citations

Epidemiological and functional implications of molecular variants of human papillomavirus

Sichero¹,

Villa²

2006

Braz J Med Biol Res

View full text Add to dashboard Cite

Human papillomavirus genomes are classified into molecular variants when they present more than 98% of similarity to the prototype sequence within the L1 gene. Comparative nucleotide sequence analyses of these viruses have elucidated some features of their phylogenetic relationship. In addition, human papillomavirus intratype variability has also been used as an important tool in epidemiological studies of viral transmission, persistence and progression to clinically relevant cervical lesions. Until the present, little has been published concerning the functional significance of molecular variants. It has been shown that nucleotide variability within the long control region leads to differences in the binding affinity of some cellular transcriptional factors and to the enhancement of the expression of E6 and E7 oncogenes. Furthermore, in vivo and in vitro studies revealed differences in E6 and E7 biochemical and biological properties among molecular variants. Nevertheless, further correlation with additional functional information is needed to evaluate the significance of genome intratypic variability. These results are also important for the development of vaccines and to determine the extent to which immunization with L1 virus-like particles of one variant could induce antibodies that cross-neutralize other variants. Key words Papillomavirus heterogeneityThe first evidence for the existence of different human papillomavirus (HPV) types was obtained in the early 1970's when it was observed that mRNA purified from plantar warts hybridized with DNA purified from other plantar warts but not from warts at other sites, including anogenital ones. In the following years, the genetic heterogeneity of HPV was confirmed by restriction fragment length polymorphism analysis and this was followed by an extensive and clear definition of HPV types based on DNA sequencing of different genes and the long control region (LCR) (1).HPV types are defined as a viral genome with an L1 late gene sequence that is at least 10% dissimilar from that of any other type. To date more than 120 different HPV genotypes associated with infections in humans have been described. In addition, the Papillomavirus Nomenclature Committee has established that HPV genomes be classified into molecular variants when they present more than 98% of similarity to the prototype

show abstract

Section: Nucleotide Variability and Risk Of Cervical Neoplasiasupporting

confidence: 57%

Section: Nucleotide Variability and Risk Of Cervical Neoplasiamentioning

confidence: 53%

Section: Papillomavirus Heterogeneitymentioning

confidence: 99%

Section: Nucleotide Variability and Risk Of Cervical Neoplasiamentioning

confidence: 99%

See 2 more Smart Citations

Epidemiological and functional implications of molecular variants of human papillomavirus

Sichero¹,

Villa²

2006

Braz J Med Biol Res

View full text Add to dashboard Cite

show abstract

“…HPV16 and -33 are high-risk HPVs frequently detected in cervical cancer. Naturally occurring variants of HPV16 and -33 with polymorphisms in the E6 open reading frame (ORF) have been reported (18,22,32,58,72), some of which have been linked to oncogenicity (23,47,73). To investigate whether these polymorphisms have an impact on p53 degradation by E6, we performed p53 expression assays in the presence of HPV16 E6 variants R10G, L83V, and R10G/L83V, as well as HPV33 E6 K35N (N), K35N/N86H (NH), I73L/V83L/K93N/ A138V (LLNV), P36T/I73L/V83L/K93N/A138V (TLLNV), and A18V/P36T/I73L/V83L/K93N/A138V (VTLLNV) and the prototype HPV16 E6 and HPV33 E6 (Fig.…”

Section: Resultsmentioning

confidence: 99%

p53 Degradation Activity, Expression, and Subcellular Localization of E6 Proteins from 29 Human Papillomavirus Genotypes

Mésplède

Gagnon

Bergeron-Labrecque

et al. 2012

J Virol

Self Cite

View full text Add to dashboard Cite

Human papillomaviruses (HPVs) are the etiological agents of cervical cancer and other human malignancies. HPVs are classified into high-and low-risk genotypes according to their association with cancer. Host cell transformation by high-risk HPVs relies in part on the ability of the viral E6 protein to induce the degradation of p53. We report the development of a cellular assay that accurately quantifies the p53 degradation activity of E6 in vivo, based on the fusion of p53 to Renilla luciferase (RLuc-p53). This assay was used to measure the p53 degradation activities of E6 proteins from 29 prevalent HPV types and variants of HPV type 16 (HPV16) and HPV33 by determining the amount of E6 expression vector required to reduce by half the levels of RLuc-p53 (50% effective concentration [EC 50 ]). These studies revealed an unexpected variability in the p53 degradation activities of different E6 proteins, even among active types whose EC 50 s span more than 2 log units. Differences in activity were greater between types than between variants and did not correlate with differences in the intracellular localization of E6, with most being predominantly nuclear. Protein and mRNA expression of the 29 E6 proteins was also examined. For 16 high-risk types, spliced transcripts that encode shorter E6*I proteins of variable sizes and abundances were detected. Mutation of the splice donor site in five different E6 proteins increased their p53 degradation activity, suggesting that mRNA splicing can limit the activity of some highrisk E6 types. The quantification of p53 degradation in vivo represents a novel tool to systematically compare the oncogenic potentials of E6 proteins from different HPV types and variants.

show abstract

Patterns of persistent genital human papillomavirus infection among women worldwide: A literature review and meta-analysis

et al. 2012

View full text Add to dashboard Cite

Persistent high-risk human papillomavirus (HR-HPV) infection is the strongest risk factor for high-grade cervical precancer. We performed a systematic review and meta-analysis of HPV persistence patterns worldwide. Medline and ISI Web of Science were searched through January 1, 2010 for articles estimating HPV persistence or duration of detection. Descriptive and meta-regression techniques were used to summarize variability and the influence of study definitions and characteristics on duration and persistence of cervical HPV infections in women. Among 86 studies providing data on over 100,000 women, 73% defined persistence as HPV positivity at a minimum of two time points. Persistence varied notably across studies and was largely mediated by study region and HPV type, with HPV-16, 31, 33 and 52 being most persistent. Weighted median duration of any-HPV detection was 9.8 months. HR-HPV (9.3 months) persisted longer than low-risk HPV (8.4 months), and HPV-16 (12.4 months) persisted longer than HPV-18 (9.8 months). Among populations of HPV positive women with normal cytology, the median duration of any-HPV detection was 11.5 and HR-HPV detection was10.9 months. In conclusion, we estimated that approximately half of HPV infections persist past 6–12 months. Repeat HPV testing at 12 month intervals could identify women at increased risk of high-grade cervical precancer due to persistent HPV infections.

show abstract

Viral Polymorphism in Human Papillomavirus Types 33 and 35 and Persistent and Transient Infection in the Genital Tract of Women

Abstract: HPV-33 and -35 polymorphism was different between types and was associated with persistence of HPV infection.

Cited by 37 publications

References 41 publications

Epidemiological and functional implications of molecular variants of human papillomavirus

Epidemiological and functional implications of molecular variants of human papillomavirus

p53 Degradation Activity, Expression, and Subcellular Localization of E6 Proteins from 29 Human Papillomavirus Genotypes

Patterns of persistent genital human papillomavirus infection among women worldwide: A literature review and meta-analysis

Contact Info

Product

Resources

About