Viral Proteins Involved in the Adsorption Process of Deep-Purple, a Siphovirus Infecting Members of the Bacillus cereus Group

Leprince, Audrey; Nuytten, Manon; Mahillon, Jacques

doi:10.1128/aem.02478-21

Cited by 4 publications

(4 citation statements)

References 73 publications

(80 reference statements)

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“…Interestingly, particular Dit proteins, called evolved Dit (evoDit) proteins, have been identified in several phages. Besides their architectural role, these proteins are also implicated in phage adsorption as they harbor CBM able to bind to the host polysaccharides [ 121 , 122 ]. By being more exposed, evoDit proteins are thought to be involved in the first reversible binding, probably to increase the possibilities of host attachment in any virion orientations [ 116 ].…”

Section: The Phage Sidementioning

confidence: 99%

“…Early characterization of the first tail protein, gp14 (YP_338197.1), revealed that it is involved in the phage γ adsorption and it was assumed that this protein was a tail fiber [ 170 ]. However, our recent analysis of the tail proteins of various siphoviruses infecting the B. cereus group (including those targeting B. anthracis ) showed that gp14 possesses a central CBM and is in fact an evolved Dit [ 122 ]. As for the second tail protein encoded by phage γ, gp15, its implication in the adsorption process was not assessed but analysis of different γ isolates highlighted that the gene encoding this protein is a hot spot for mutations, consistent with its involvement in phage adsorption [ 170 ].…”

Section: Adsorption Of Phages Infecting Gram-positive Bacteriamentioning

confidence: 99%

“…Regarding phages infecting other members of the B. cereus group, several studies showed that they probably rely on carbohydrates for their adsorption, but the exact nature of these receptors has never been demonstrated [ 171 , 172 ]. Recently, we performed an in silico analysis of the tail proteins found in siphoviruses infecting the B. cereus group [ 122 ]. Five different genetic organizations were identified in these phages with the most prevalent ones displaying similar gene syntenies to B. subtilis phage SPP1 (organization A) and L. lactis phage p2 (organization B).…”

Section: Adsorption Of Phages Infecting Gram-positive Bacteriamentioning

confidence: 99%

“…It is noteworthy that CBM are widespread in Dit protein, Tal, and Tal/RBP found in B. cereus phages, giving further credence to the implication of carbohydrates in the adsorption of B. cereus phages. It was also experimentally shown that in the case of the siphophage Deep-Purple, the CBM found in the evoDit protein (i.e., Gp28) and in the Tal/RBP (i.e., Gp29) are both involved in the phage adsorption as GFP fused CBM were able to bind to B. cereus cells, although with different binding ranges [ 122 ]. Indeed, while the evoDit protein bound to sensitive strains but also some insensitive ones, the Tal/RBP could only bind to sensitive strains.…”

Section: Adsorption Of Phages Infecting Gram-positive Bacteriamentioning

confidence: 99%

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Phage Adsorption to Gram-Positive Bacteria

Leprince

Mahillon

2023

Viruses

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The phage life cycle is a multi-stage process initiated by the recognition and attachment of the virus to its bacterial host. This adsorption step depends on the specific interaction between bacterial structures acting as receptors and viral proteins called Receptor Binding Proteins (RBP). The adsorption process is essential as it is the first determinant of phage host range and a sine qua non condition for the subsequent conduct of the life cycle. In phages belonging to the Caudoviricetes class, the capsid is attached to a tail, which is the central player in the adsorption as it comprises the RBP and accessory proteins facilitating phage binding and cell wall penetration prior to genome injection. The nature of the viral proteins involved in host adhesion not only depends on the phage morphology (i.e., myovirus, siphovirus, or podovirus) but also the targeted host. Here, we give an overview of the adsorption process and compile the available information on the type of receptors that can be recognized and the viral proteins taking part in the process, with the primary focus on phages infecting Gram-positive bacteria.

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Section: The Phage Sidementioning

confidence: 99%