Viral Respiratory Infections: New Tools for a Rapid Diagnosis

Colagrossi, Luna; Mattana, Giordana; Piccioni, Livia; Cento, Valeria; Perno, Carlo Federico

doi:10.1055/s-0041-1739306

Cited by 5 publications

(4 citation statements)

References 112 publications

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“…Respiratory tract infections in children are often associated with respiratory pathogens, including measles virus, influenza virus, varicella zoster virus, coronavirus, respiratory syncytial virus, adenovirus, Bordetella pertussis , diphtheria, Haemophilus influenzae , Mycobacterium tuberculosis, Neisseria meningitidis, Streptococcus pneumoniae , Mycoplasma pneumoniae , and Chlamydia pneumoniae ( 10 , 12 – 14 ). A variety of methods can be applied in clinical pathogen detection, and molecular detection methods are the most widely used ( 15 ).…”

Section: Discussionmentioning

confidence: 99%

Multicenter evaluation of the Acaruiter Respiratory Panel for diagnosis of respiratory tract infections in Chinese children

Zhao,

Dong,

Cao

et al. 2023

Microbiol Spectr

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Respiratory tract infection represents the most frequent infectious disease afflicting children worldwide. This study was to evaluate the performance of Acaruiter Respiratory Panel (fluorescent PCR) in the detection of respiratory tract infection pathogens in children. Clinical trials were conducted in three representative pediatric hospitals in Shanghai, Chengdu, and Beijing. With reference to another widely used multiplex assay and first-generation sequencing, the sensitivity, specificity, accuracy, and consistency coefficient kappa ( κ ) value of the assay were evaluated to verify the accuracy and effectiveness in the detection of clinical respiratory pathogens, so as to objectively evaluate the clinical performance and application value of Acaruiter Respiratory Panel (fluorescent PCR). A total of 1,540 samples were screened in this clinical trial project, of which 1,404 were valid samples. Based on the comprehensive analysis of the clinical trial data of 1,404 effective samples, the detection ability of Acaruiter Respiratory Panel (fluorescent PCR) for various pathogens compared with the results of clinical diagnosis, the positive percent agreement was 97.04%–100%, the negative percent agreement was 99.31%–100%, the total percent agreement was 99.36%–100%, and the kappa value was 0.982–1.000, which had good consistency and high accuracy. IMPORTANCE Compared to multiplex PCR assays that are available in the Chinese market, the Acaruiter Respiratory Panel (fluorescent PCR) covers a wider range of pathogens including eight viruses, five bacteria, Mycoplasma pneumoniae and Chlamydia pneumoniae , and has high accuracy and effectiveness in the detection of pathogens. This is the first study to evaluate the performance of the Acaruiter Respiratory Panel. This regent may be a promising assay for comprehensive testing for respiratory pathogens from nasopharyngeal swab specimens in Chinese children.

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Section: Discussionmentioning

confidence: 99%

Multicenter evaluation of the Acaruiter Respiratory Panel for diagnosis of respiratory tract infections in Chinese children

Zhao,

Dong,

Cao

et al. 2023

Microbiol Spectr

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“…Finally, the nasopharyngeal swab is a reliable "proxy" for the possible presence of the virus in the lower tracts, which are the "best habitat" for Parainfluenza virus 3, Metapneumovirus, and Influenza virus, but not for Rhinoviruses (which represent the most frequent co-infection in this cohort of patients), as well as for Coronaviruses (OC43 and 229E) (30). Indeed, these last have an optimum of growth at the cool temperatures found in the nasal cavity (33-35°C) than at core body temperature (37°C) (31), and thus they may not be present in replicating form in the lower airways unless the patient is subjected to invasive ventilation (in this case, Rhinoviruses are the first cause of viral pneumonia) (32).…”

Section: Patients (N = 60)mentioning

confidence: 93%

Are lung ultrasound features more severe in infants with bronchiolitis and coinfections?

De Rose,

Maddaloni,

Martini

et al. 2023

Front. Pediatr.

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BackgroundThe lung ultrasound (LUS) score can be a useful tool to predict the need for respiratory support and the length of hospital stay in infants with bronchiolitis.ObjectiveTo compare lung ultrasound features in neonates and infants up to three months of age with bronchiolitis to determine whether LUS scores (range 0–36) differ in infants with coinfections or not.MethodsNeonates and infants younger than three months admitted to neonatal units from October 2022 to March 2023, who underwent lung ultrasound evaluation on admission, were included in this retrospective study.ResultsWe included 60 patients who underwent LUS evaluation at admission. Forty-two infants (70.0%) had a single viral infection. Eighteen infants (30.0%) had a coinfection: fifteen infants (25.0%) had more than one virus at PCR; one infant (1.7%) had both a viral coinfection and a viral-bacteria coinfection; two infants (3.3%) had viral-bacteria coinfection. Infants with a single viral infection and those with coinfections had similar LUS scores globally and in different lung zones. An LUS score higher than 8 was identified to significantly predict the need for any respiratory support (p = 0.0035), whereas an LUS score higher than 13 was identified to significantly predict the need for mechanical ventilation (p = 0.024).ConclusionIn our small cohort of neonates and infants younger than three months hospitalized with bronchiolitis, we found no statistically significant differences in the LUS score on admission between patients with a single viral infection and those with multiple infections.

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“…Two review articles provided general overviews of the microbiologic diagnosis of pneumonia, including important data about microbiologic diagnostic testing for respiratory viruses that cause pneumonia [116,117]. A recent review by COLAGROSSI et al [118] provided an overview of the rapid microbiologic detection of the main respiratory viruses.…”

Section: Microbiologic Diagnosismentioning

confidence: 99%

“…A recent review by C olagrossi et al . [ 118 ] provided an overview of the rapid microbiologic detection of the main respiratory viruses.…”

Section: Microbiologic Diagnosismentioning

confidence: 99%

Respiratory viruses: their importance and lessons learned from COVID-19

et al. 2022

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Respiratory virus infection can cause severe illnesses capable of inducing acute respiratory failure that can progress rapidly to acute respiratory distress syndrome (ARDS). ARDS is related to poor outcomes, especially in individuals with a higher risk of infection, such as the elderly and those with comorbidities,i.e.obesity, asthma, diabetes mellitus and chronic respiratory or cardiovascular disease. Despite this, effective antiviral treatments available for severe viral lung infections are scarce. The coronavirus disease 2019 (COVID-19) pandemic demonstrated that there is also a need to understand the role of airborne transmission of respiratory viruses. Robust evidence supporting this exists, but better comprehension could help implement adequate measures to mitigate respiratory viral infections. In severe viral lung infections, early diagnosis, risk stratification and prognosis are essential in managing patients. Biomarkers can provide reliable, timely and accessible information possibly helpful for clinicians in managing severe lung viral infections. Although respiratory viruses highly impact global health, more research is needed to improve care and prognosis of severe lung viral infections. In this review, we discuss the epidemiology, diagnosis, clinical characteristics, management and prognosis of patients with severe infections due to respiratory viruses.

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Viral Respiratory Infections: New Tools for a Rapid Diagnosis

Cited by 5 publications

References 112 publications

Multicenter evaluation of the Acaruiter Respiratory Panel for diagnosis of respiratory tract infections in Chinese children

Multicenter evaluation of the Acaruiter Respiratory Panel for diagnosis of respiratory tract infections in Chinese children

Are lung ultrasound features more severe in infants with bronchiolitis and coinfections?

Respiratory viruses: their importance and lessons learned from COVID-19

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