Viral RNA‐dependent DNA polymerase RNA‐dependent DNA polymerase in virions of Rous sarcoma virus

Weiss, Robin A.

doi:10.1002/(sici)1099-1654(199801/03)8:1<3::aid-rmv218>3.0.co;2-#

Cited by 5 publications

(4 citation statements)

References 28 publications

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“…In contrast, TFs with rve and RVT domains occur only in N. vitripennis . These domains were shown to be necessary for most retrotransposons and retroviruses 14 15 . The possible degradation of retrotransposons in genomes of C. solmsi and A. mellifera may owe to the loss of their rve and RVT domains 10 16 .…”

Section: Resultsmentioning

confidence: 99%

Regulation of transcription factors on sexual dimorphism of fig wasps

Sun

Jia

et al. 2015

Sci Rep

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Fig wasps exhibit extreme intraspecific morphological divergence in the wings, compound eyes, antennae, body color, and size. Corresponding to this, behaviors and lifestyles between two sexes are also different: females can emerge from fig and fly to other fig tree to oviposit and pollinate, while males live inside fig for all their lifetime. Genetic regulation may drive these extreme intraspecific morphological and behavioral divergence. Transcription factors (TFs) involved in morphological development and physiological activity may exhibit sex-specific expressions. Herein, we detect 865 TFs by using genomic and transcriptomic data of the fig wasp Ceratosolen solmsi. Analyses of transcriptomic data indicated that up-regulated TFs in females show significant enrichment in development of the wing, eye and antenna in all stages, from larva to adult. Meanwhile, TFs related to the development of a variety of organs display sex-specific patterns of expression in the adults and these may contribute significantly to their sexual dimorphism. In addition, up-regulated TFs in adult males exhibit enrichment in genitalia development and circadian rhythm, which correspond with mating and protandry. This finding is consistent with their sex-specific behaviors. In conclusion, our results strongly indicate that TFs play important roles in the sexual dimorphism of fig wasps.

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Section: Resultsmentioning

confidence: 99%

Regulation of transcription factors on sexual dimorphism of fig wasps

Sun

Jia

et al. 2015

Sci Rep

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“…The higher amplification temperature also permitted more precise targeting of the DNA and reduced the incidence of primer dimers. Combined with the in vitro development of reverse transcription (RT) of mRNA into complementary DNA (cDNA) [ 23 , 24 , 25 , 26 ], detailed investigations of target transcripts became feasible. In order to visualize the PCR product(s), the samples were separated using gel-electrophoresis [ 27 , 28 , 29 ].…”

Section: Historical Background—nucleic Acid Amplificationmentioning

confidence: 99%

Single-Cell Isolation and Gene Analysis: Pitfalls and Possibilities

Hodne

Weltzien

2015

IJMS

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During the last two decades single-cell analysis (SCA) has revealed extensive phenotypic differences within homogenous cell populations. These phenotypic differences are reflected in the stochastic nature of gene regulation, which is often masked by qualitatively and quantitatively averaging in whole tissue analyses. The ability to isolate transcripts and investigate how genes are regulated at the single cell level requires highly sensitive and refined methods. This paper reviews different strategies currently used for SCA, including harvesting, reverse transcription, and amplification of the RNA, followed by methods for transcript quantification. The review provides the historical background to SCA, discusses limitations, and current and future possibilities in this exciting field of research.

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“…Retroviruses are a family of RNA enveloped viruses with the ability to`reverse transcribe' their genome from RNA to DNA [3]. Virions measure 120 nm in diameter and contain two identical copies of positive strand RNA genome complexed with nucleocapsid proteins.…”

Section: The Virus Particlementioning

confidence: 99%

Progress with retroviral gene vectors

et al. 2000

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Retroviral vectors have become a standard tool for gene transfer technology. Compared with other gene transfer systems, retroviral vectors have several advantages, including their ability to transduce a variety of cell types, to integrate efficiently into the genomic DNA of the recipient cells and to express the transduced gene at high levels. The relatively well understood biology of retroviruses has made possible the development of packaging cell lines which provide in trans all the viral proteins required for viral particle formation. The design of different types of packaging cells has evolved to reduce the possibility of helper virus production. The host range of retroviruses has been expanded by pseudotyping the vectors with heterologous viral glycoproteins and receptor‐specific ligands. The development of lentivirus vectors has allowed efficient gene transfer to quiescent cells. This review describes different strategies adopted for developing vectors to be used in gene therapy applications. Copyright © 2000 John Wiley & Sons, Ltd.

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Viral RNA‐dependent DNA polymerase RNA‐dependent DNA polymerase in virions of Rous sarcoma virus

Cited by 5 publications

References 28 publications

Regulation of transcription factors on sexual dimorphism of fig wasps

Regulation of transcription factors on sexual dimorphism of fig wasps

Single-Cell Isolation and Gene Analysis: Pitfalls and Possibilities

Progress with retroviral gene vectors

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