Viral surveillance using PCR during treatment of AML and ALL

Dixon, Stephanie B; Lane, Adam; O’Brien, Maureen M.; Burns, Karen; Mangino, Jennifer; Breese, Erin H.; Absalon, Michael J.; Perentesis, John P.; Phillips, Christine L

doi:10.1002/pbc.26752

Cited by 10 publications

(16 citation statements)

References 25 publications

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“…In the current study, HSV and VZV DNA was not detected in plasma samples indicating that DNAemia are not common in this group of patients suggesting that DNAemia might be rarely observed in recurrent infections in immunocompromised hosts [22,23]. Patients who are present from geographic regions with a high seroprevalence of either virus in both developing and developed countries are at a higher risk of viral reactivation and symptomatic and asymptomatic screening should be tailored to the present increased risk [8].…”

Section: Discussionmentioning

confidence: 49%

“…The median follow-up duration was 7.4 months range (1-36) from the start of chemotherapy. There are 734 blood specimens, (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) samples per patient with a median of seven samples per patient from which all plasma samples were isolated and stored at − 80°C until DNA extraction. The study was approved by the Ethics Committee and Medical Research of Farhat Hached University Hospital of Sousse, ref: IRB registration number assigned by OHRP: IRB00008931 and informed consent was obtained from all participants enrolled in the study.…”

Section: Study Design and Participantsmentioning

confidence: 99%

“…Similarly, in these settings, poor data is available concerning the other HHVs which can also lead to severe diseases. Most existing studies consist of retrospective analyses and case series whereas only limited information is available from prospective studies [2,[4][5][6][7][8]. The reported incidence range and outcomes of herpes viral infections among these patients groups are wide and variable due to a number of factors, including the study design, the type of disease, the intensity and duration of T-cell-mediated immune suppression [9].…”

Section: Introductionmentioning

confidence: 99%

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Co-infections of human herpesviruses (CMV, HHV-6, HHV-7 and EBV) in non-transplant acute leukemia patients undergoing chemotherapy

Handous

Achour

Marzouk

et al. 2020

Virol J

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Background: Human herpesviruses (HHVs) remain latent after primary infection and can be reactivated in response to immunosuppression and chemotherapy. Little is known about their incidence, potential relationships, risk factors and clinical impact in non-transplant leukemia patients. This study investigated prospectively incidence, risk factors, clinical impact and possible association of HHVs-(1-7) infections in patients with newly diagnosed acute leukemia. Methods: Study design involved longitudinal sampling before chemotherapy and in different phases of chemotherapy: post-induction, post-remission, and post-salvage during 2016-2018. A total of 734 plasma samples from 95 patients were analyzed by a qualitative, multiplex PCR for HHVs detection and a quantitative real-time PCR was used for cytomegalovirus (CMV) quantification. HHVs-(1-6) IgG and IgM antibodies were tested using immunoassays. Risk factors were analyzed by binary logistic regression and relationships between viruses were analyzed using the Chi-square or Fisher's exact test as appropriate. Results: The overall seroprevalences of HHV-(1-6) IgG were high (> 80%). At least one herpes viral agent was detected in 60 patients (63.3%). CMV was the most commonly detected virus in the different phases of chemotherapy (19.4%), followed by HHV-6 (9.7%), HHV-7 (5.2%) and EBV (2.7%). HSV-1/2 and VZV DNA were not detected. Twenty-seven patients (28.4%) had more than one virus detected in the follow-up, with 23 who were co-infected. CMV/HHV-6 was the most frequent co-infection (69.5%, 16/23). HHV-6 infection (p = 0.008) was identified as a risk factor for CMV infection while salvage treatment (p = 0.04) and CMV infection (p = 0.007) were found to be independent risk factors for HHV-6 infection. CMV co-infection was associated with severe lymphopenia with an absolute lymphocyte count (ALC) (< 500/μL) (p = 0.009), rash (p = 0.011), pneumonia (p = 0.016) and opportunistic infections [bacteremia, p < 0.001 and invasive fungal infection, (p = 0.024)] more frequently than CMV mono-viral infections.

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Section: Discussionmentioning

confidence: 49%

Section: Study Design and Participantsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Co-infections of human herpesviruses (CMV, HHV-6, HHV-7 and EBV) in non-transplant acute leukemia patients undergoing chemotherapy

Handous

Achour

Marzouk

et al. 2020

Virol J

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“…Comprehensive EV testing was performed for a period of 17 years in pediatric hematologic and oncologic patients including but not restricted to recipients of HSCT . In contrast to case reports of EV infections and reports on small outbreaks in this group of patients, we analyzed a tremendous number of specimens and patients and detected EV RNA, nonetheless, very rarely.…”

Section: Discussionmentioning

confidence: 99%

Enterovirus infections in pediatric hematologic/oncologic patients

Strenger

Kessler

Stelzl

et al. 2018

Pediatric Blood & Cancer

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Background: Enteroviruses (EV) are a large group of Picornaviruses associated with respiratory, gastrointestinal, and neurologic symptoms in the immunocompetent host. Little is known about the epidemiologic and clinical impact in pediatric hematologic/oncologic patients. Procedure: From 2001 through 2017, different clinical specimens were collected from pediatric hematologic/oncologic patients and were tested for enteroviral RNA.Results: Of 13 004 specimens collected from 761 patients, 38 (0.3%) obtained from 14 patients(1.8%) tested positive for EV RNA. Viral shedding was observed without viremia and vice versa.None of 80 cerebrospinal fluid specimens obtained from 60 patients with neurologic symptoms were positive for EV RNA. None of 14 patients positive for EV RNA showed EV-specific symptoms.In 11/14 patients, EV RNA was found to be negative in the follow-up specimen. The remaining patient with a severe primary immune deficiency showed repeated positive EV RNA results for >5 years. Conclusions:In this pediatric hematologic/oncologic cohort, EV infection occurred rarely and without related symptoms. Specimens concurrently obtained from one patient are commonly not in accordance with each other. In the vast majority of patients, EV RNA appears to turn negative in the follow-up specimen. EV infections seem to have a low impact in this patient cohort. K E Y W O R D Senterovirus, hematopoietic stem cell transplantation, immunodeficiency, pediatric hematologic/oncologic patients, picornavirus

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“…Abbreviations: ADV, adenovirus; ALL, acute lymphoblastic leukemia; AML, acute myeloid leukemia; CMV, cytomegalovirus; HSCT, hematopoietic stem cell transplantation; HSV, herpes simplex virus; PCR, polymerase chain reaction; PVB 19, parvovirus B19; VZV, varicella zoster virus control is an important aspect of supportive cancer care. However, data on the occurrence of viremia of common viral pathogens are scarce and based on small and retrospective studies, 8,9 and information on symptomatic viral infections is mostly limited to case reports.…”

Section: And Theirmentioning

confidence: 99%

Systemic viral infection in children receiving chemotherapy for acute leukemia

Buus-Gehrig

Bochennek

Hennies

et al. 2020

Pediatric Blood & Cancer

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Systemic viral diseases frequently occur in allogeneic hematopoietic stem cell transplantation, but data in children receiving chemotherapy for acute leukemia are scarce. We therefore collected and analyzed the published data on symptomatic infection from cytomegalovirus, herpes simplex virus, varicella zoster virus, parvovirus B19, or adenovirus in pediatric acute leukemia. Reports on 68 children were identified, of whom 16 patients have died from the infection. Further studies have to (1) evaluate the true incidence of these infections in pediatric acute leukemia, (2) their impact on outcome, and (3) whether a subpopulation of patients could benefit from screening and prophylactic strategies. K E Y W O R D S acute leukemia, adenovirus, child, cytomegalovirus, herpes simplex virus, parvovirus B19, systemic viral disease, varicella zoster virus We performed a MEDLINE (including MEDLINE In Process) search to identify relevant English-language articles indexed from January 1, 2000, up to December 31, 2019. The search included a combination of indexed terms and free text terms (child OR adolescent OR pediatr*); (cancer OR malignancy OR leukemia); (parvovirus OR b19)/(adenovirus)/(varicella or VZV)/(cytomegalovirus or CMV)/(herpes simplex virus or HSV). The hits were screened manually by two of the authors (CBG and TL). Only case reports and case series about systemic infections or studies on the prevalence of viremia with CMV, HSV, VZV, PVB19, and ADV, respectively, occurring in children

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Viral surveillance using PCR during treatment of AML and ALL

Cited by 10 publications

References 25 publications

Co-infections of human herpesviruses (CMV, HHV-6, HHV-7 and EBV) in non-transplant acute leukemia patients undergoing chemotherapy

Co-infections of human herpesviruses (CMV, HHV-6, HHV-7 and EBV) in non-transplant acute leukemia patients undergoing chemotherapy

Enterovirus infections in pediatric hematologic/oncologic patients

Systemic viral infection in children receiving chemotherapy for acute leukemia

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