Viral transcription in human keratinocyte cell lines immortalized by human papillomavirus type-16

Oncogenes and tumor angiogenesis: the HPV-16 E6 oncoprotein activates the vascular endothelial growth factor (VEGF) gene promoter in a p53 independent manner

“…C33A is a HPVnegative cervical cancer cell line, while HeLa and CaSki are both HPV-16-positive. HPK1A cells have been previously described (Rohlfs et al, 1991;Durst et al, 1987) and were generated by exogenous expression of HPV-16 DNA into human primary foreskin keratinocytes. All cells were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA) with the exception of MEF cells, which were a generous gift from Dr Larry Donehower, and the HPK1A cell line, which was kindly provided by Dr Federico DeMarco.…”

Human papillomavirus (HPV) type 16 and 33 E6/E7 region transcripts in tonsillar carcinomas can originate from integrated and episomal HPV DNA

“…Since the HPV E20RF encodes transcriptional modulator proteins Cripe et al, 1987;Bernard et al, 1989;Romanczuk et al, 1990), it has been suggested that integration-mediated disruption of E2 expression would trigger uncontrolled expression of the transforming genes E6 and E7. Another event often resulting from HPV-16 or HPV-18 DNA integration is E6/E7 expression via virus-cell fusion transcripts (Inagaki et al, 1988;Schneider-G/idicke & Schwarz, 1986;Rohlfs et al, 1991 ;Smits et al, 1991).…”

Os papilomavírus humanos – HPV: histórico, morfologia e ciclo biológico

“…Estudos da transcrição viral em linhagens de queratinócitos humanos, imortalizados por HPV-16 (Doorbar et al, 1990;Rohls et al, 1991), mostraram que os genes precoces são transcritos a partir de promotores, por splicing diferencial. Assim, vários RNAs são produzidos a partir de promotores diferentes.…”