Viral Vectors, Exosomes, and Vexosomes: Potential armamentarium for delivering CRISPR/Cas to cancer cells

Farzanehpour, Mahdieh; Miri, Ali; Alvanegh, Akbar Ghorbani; Gouvarchinghaleh, Hadi Esmaeili

doi:10.1016/j.bcp.2023.115555

Cited by 15 publications

(9 citation statements)

References 179 publications

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“…Viral vectors are genetically modified viruses that can deliver therapeutic genes or drugs to cancer cells. Compared to viral vectors, exosomes do not pose the risk of causing an immune response or integrating into the host genome (Farzanehpour et al, 2023; Xu et al, 2021). Moreover, exosomes show little long‐term accumulation in tissues, which results in low immunogenicity and toxicity (Kalluri & LeBleu, 2020).…”

Section: Exosome‐based Nanoparticle and Ncrna Deliverymentioning

confidence: 99%

Applications of engineered exosomes in drugging noncoding RNAs for cancer therapy

et al. 2023

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Noncoding RNAs (ncRNAs) are engaged in key cell biological and pathological events, and their expression alteration is connected to cancer progression both directly and indirectly. A huge number of studies have mentioned the significant role of ncRNAs in cancer prevention and therapy that make them an interesting subject for cancer therapy. However, there are several limitations, including delivery, uptake, and short half‐life, in the application of ncRNAs in cancer treatment. Exosomes are introduced as promising options for the delivery of ncRNAs to the target cells. In this review, we will briefly discuss the application and barriers of ncRNAs. After that we will focus on exosome‐based ncRNAs delivery and their advantages as well as the latest achievements in drugging ncRNAs with exosomes.

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Section: Exosome‐based Nanoparticle and Ncrna Deliverymentioning

confidence: 99%

Applications of engineered exosomes in drugging noncoding RNAs for cancer therapy

et al. 2023

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“…Exosomes, a subset of the extracellular vesicles (EVs), have recently gained attention as vectors due to the plethora of natural sources from which they can be harvested and/or cultured. − Exosomes are 50–200 nm in size with a phospholipid bilayer that encapsulates biomolecular contents from the host cell of origin. Due to their makeup, exosomes are intimately involved in cellular communication ,, and thus can be used as vectors, being proven to be capable of tissue-specific delivery and crossing biological barriers, such as the blood brain barrier. , In comparison to current vectors, exosomes are advantageous due to their biocompatibility, easy uptake without negative immune responses, lower toxicity, and selectivity toward target cancer cells . Exosomes have been used to treat various cancers ranging from ovarian to pancreatic cancers, − with tumor-derived exosomes proven to target the cell-types of origin .…”

mentioning

confidence: 99%

“…Due to their makeup, exosomes are intimately involved in cellular communication ,, and thus can be used as vectors, being proven to be capable of tissue-specific delivery and crossing biological barriers, such as the blood brain barrier. , In comparison to current vectors, exosomes are advantageous due to their biocompatibility, easy uptake without negative immune responses, lower toxicity, and selectivity toward target cancer cells . Exosomes have been used to treat various cancers ranging from ovarian to pancreatic cancers, − with tumor-derived exosomes proven to target the cell-types of origin . Recent studies have also looked at engineering exosomes for the targeted transport of their contents. , At present, a number of different cell cultivation approaches are under investigation for mass production, including those derived from human mesenchymal stem cells (MSCs) and embryonic kidney (HEK) cells as well as CHO cells .…”

mentioning

confidence: 99%

“…25,27 In comparison to current vectors, exosomes are advantageous due to their biocompatibility, easy uptake without negative immune responses, lower toxicity, and selectivity toward target cancer cells. 28 Exosomes have been used to treat various cancers ranging from ovarian to pancreatic cancers, 29−33 with tumor-derived exosomes proven to target the cell-types of origin. 28 Recent studies have also looked at engineering exosomes for the targeted transport of their contents.…”

mentioning

confidence: 99%

“…28 Exosomes have been used to treat various cancers ranging from ovarian to pancreatic cancers, 29−33 with tumor-derived exosomes proven to target the cell-types of origin. 28 Recent studies have also looked at engineering exosomes for the targeted transport of their contents. 26,34 At present, a number of different cell cultivation approaches are under investigation for mass production, including those derived from human mesenchymal stem cells (MSCs) and embryonic kidney (HEK) cells 35 as well as CHO cells.…”

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confidence: 99%

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Quantitative Recoveries of Exosomes and Monoclonal Antibodies from Chinese Hamster Ovary Cell Cultures by Use of a Single, Integrated Two-Dimensional Liquid Chromatography Method

Wysor,

Marcus

2023

Anal. Chem.

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Recent Progress in Prompt Molecular Detection of Exosomes Using CRISPR/Cas and Microfluidic‐Assisted Approaches Toward Smart Cancer Diagnosis and Analysis

Mojtaba Mousavi,

Alireza Hashemi,

Yari Kalashgrani

et al. 2023

ChemMedChem

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Exosomes are essential indicators of molecular mechanisms involved in interacting with cancer cells and the tumor environment. As nanostructures based on lipids and nucleic acids, exosomes provide a communication pathway for information transfer by transporting biomolecules from the target cell to other cells. Importantly, these extracellular vesicles are released into the bloodstream by the most invasive cells, i. e., cancer cells; in this way, they could be considered a promising specific biomarker for cancer diagnosis. In this matter, CRISPR‐Cas systems and microfluidic approaches could be considered practical tools for cancer diagnosis and understanding cancer biology. CRISPR‐Cas systems, as a genome editing approach, provide a way to inactivate or even remove a target gene from the cell without affecting intracellular mechanisms. These practical systems provide vital information about the factors involved in cancer development that could lead to more effective cancer treatment. Meanwhile, microfluidic approaches can also significantly benefit cancer research due to their proper sensitivity, high throughput, low material consumption, low cost, and advanced spatial and temporal control. Thereby, employing CRISPR‐Cas‐ and microfluidics‐based approaches toward exosome monitoring could be considered a valuable source of information for cancer therapy and diagnosis. This review assesses the recent progress in these promising diagnosis approaches toward accurate cancer therapy and in‐depth study of cancer cell behavior.

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Viral Vectors, Exosomes, and Vexosomes: Potential armamentarium for delivering CRISPR/Cas to cancer cells

Cited by 15 publications

References 179 publications

Applications of engineered exosomes in drugging noncoding RNAs for cancer therapy

Applications of engineered exosomes in drugging noncoding RNAs for cancer therapy

Quantitative Recoveries of Exosomes and Monoclonal Antibodies from Chinese Hamster Ovary Cell Cultures by Use of a Single, Integrated Two-Dimensional Liquid Chromatography Method

Recent Progress in Prompt Molecular Detection of Exosomes Using CRISPR/Cas and Microfluidic‐Assisted Approaches Toward Smart Cancer Diagnosis and Analysis

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