Virgibacillus chiguensis sp. nov., a novel halophilic bacterium isolated from Chigu, a previously commercial saltern located in southern Taiwan

Wang, Chung-Yi; Chang, Chen-Chin; Ng, Chang Chai; Chen, Tseng-Wei; Shyu, Yuan‐Tay

doi:10.1099/ijs.0.64996-0

Cited by 49 publications

(19 citation statements)

References 15 publications

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“…in 1998 and currently consists of mainly Gram-positive, motile, spore-forming, rod-shaped bacteria that are moderately halophilic [11] . Members of the genus Virgibacillus are found in various environments including sediment of a saline lake [12] , [13] , [14] , [15] , traditional salt-fermented seafood [16] , a permafrost core collected from the Canadian high Arctic [17] , a marine solar saltern [18] , [19] , [20] , [21] , biofilm formation on mural paintings [22] , seawater [23] , [24] , field soil, a dairy product sample [25] , a saline mud sample [26] , residual wash water produced during the processing of Spanish-style green table olive sewage [27] , salt crust [28] and fermented fish [29] .…”

Section: Introductionmentioning

confidence: 99%

Noncontiguous finished genome sequence and description of Virgibacillus massiliensis sp. nov., a moderately halophilic bacterium isolated from human gut

Khelaifia

Croce

Lagier

et al. 2015

New Microbes and New Infections

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Strain Vm-5T was isolated from the stool specimen of a 10-year-old Amazonian boy. This bacterium is a Gram-positive, strictly aerobic rod, motile by a polar flagellum. Here we describe its phenotypic characteristics and complete genome sequence. The 4 353 177 bp long genome exhibits a G + C content of 36.87% and contains 4394 protein-coding and 125 predicted RNA genes. Phylogenetically and genetically, strain Vm-c is a member of the genus Virgibacillus but is distinct enough to be classified as a new species. We propose the creation of V. massiliensis sp. nov., whose type strain is strain Vm-5T (CSUR P971 = DSM 28587).

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Section: Introductionmentioning

confidence: 99%

Noncontiguous finished genome sequence and description of Virgibacillus massiliensis sp. nov., a moderately halophilic bacterium isolated from human gut

Khelaifia

Croce

Lagier

et al. 2015

New Microbes and New Infections

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“…They are Gram‐positive, endospore‐forming, catalase‐ and oxidase‐positive, motile, rod‐shaped, aerobic bacteria and grow optimally at between 5 and 25% NaCl, at pH 6·0–10·0 (optimum 7·5–8·0) and 10–55°C (optimum 30–40°C) (Hua et al. 2008; Wang et al. 2008; Chen et al.…”

Section: Introductionmentioning

confidence: 99%

Identification of novel halotolerant bacillopeptidase F-like proteinases from a moderately halophilic bacterium, Virgibacillus sp. SK37

Phrommao

Rodtong

Yongsawatdigul

2010

Journal of Applied Microbiology

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Aims: Virgibacillus sp. SK37 isolated from Thai fish sauce produced numerous NaCl‐activated subtilisin‐like proteinases. Our objectives were to purify, characterize and identify these extracellular proteinases. Methods and Results: Three major subtilisin‐like enzymes including 19, 34 and 44 kDa were partially purified and showed maximum activity at pH 8, 55–60°C, 25–30% NaCl and 70–100 mmol l−1 CaCl2. Enzymes showed stability at 0–30% NaCl and <20 mmol l−1 CaCl2 and were completely inhibited by phenylmethanesulphonyl fluoride but not by ethylenediaminetetraacetic acid. The isoelectric points of 19‐, 34‐ and 44‐kDa proteinases were at 3·6, 5·2 and 3·8, respectively, based on 2D electrophoresis. Peptide mass fingerprint and de novo peptide homology analysis of tryptic peptides using MALDI‐TOF and LC–MS/MS, respectively, suggested that all three enzymes were novel and homologous to bacillopeptidase F. Conclusions: The three major proteinases are a member of bacillopeptidase F‐like enzymes exhibiting thermophilic and halotolerant characteristics with high stability at 30% NaCl. Significance and Impact of the Study: This is the first report on bacillopeptidase F‐like proteinases in genus Virgibacillus with a distinct halotolerant feature. They showed potential to be a processing aid for food and biotechnological applications, particularly in high salt condition.

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“…One example of such independent linage is a group comprising the genera Alkalibacillus and the genus Virgibacillus [43]. In this work, two out of the 3 clones assigned to the Virgibacillus genus showed sequence similarity to the species Virgibacillus chiguensis [44] showing a high sequence similarity (98.4-98.6 %) and the other clone was phylogenetically assigned to Virgibacillus halodenitrificans (91 % sequence similarity) [45].…”

Section: Phylogenetic Analysis Of the Bacterial Clones And Dominant Pmentioning

confidence: 56%

Phylogenetic Profiling and Diversity of Bacterial Communities in the Death Valley, an Extreme Habitat in the Atacama Desert

et al. 2015

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The Atacama Desert, one of the driest deserts in the world, represents a unique extreme environmental ecosystem to explore the bacterial diversity as it is considered to be at the dry limit for life. A 16S rRNA gene (spanning the hyper variable V3 region) library was constructed from an alkaline sample of unvegetated soil at the hyperarid margin in the Atacama Desert. A total of 244 clone sequences were used for MOTHUR analysis, which revealed 20 unique phylotypes or operational taxonomic units (OTUs). V3 region amplicons of the 16S rRNA were suitable for distinguishing the bacterial community to the genus and specie level. We found that all OTUs were affiliated with taxa representative of the Firmicutes phylum. The extremely high abundance of Firmicutes indicated that most bacteria in the soil were spore-forming survivors. In this study we detected a narrower diversity as compared to other ecological studies performed in other areas of the Atacama Desert. The reported genera were Oceanobacillus (representing the 69.5 % of the clones sequenced), Bacillus, Thalassobacillus and Virgibacillus. The present work shows physical and chemical parameters have a prominent impact on the microbial community structure. It constitutes an example of the communities adapted to live in extreme conditions caused by dryness and metal concentrations.

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Virgibacillus chiguensis sp. nov., a novel halophilic bacterium isolated from Chigu, a previously commercial saltern located in southern Taiwan

Cited by 49 publications

References 15 publications

Noncontiguous finished genome sequence and description of Virgibacillus massiliensis sp. nov., a moderately halophilic bacterium isolated from human gut

Noncontiguous finished genome sequence and description of Virgibacillus massiliensis sp. nov., a moderately halophilic bacterium isolated from human gut

Identification of novel halotolerant bacillopeptidase F-like proteinases from a moderately halophilic bacterium, Virgibacillus sp. SK37

Phylogenetic Profiling and Diversity of Bacterial Communities in the Death Valley, an Extreme Habitat in the Atacama Desert

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