Virgibacillus natechei sp. nov., A Moderately Halophilic Bacterium Isolated from Sediment of a Saline Lake in Southwest of Algeria

Abstract

A novel, Gram-positive, moderately halophilic bacterium, oxidase- and catalase-positive designated FarD(T) was isolated from sediments of a saline lake located in Taghit, 93 km from Bechar, southwest of Algeria. Cells were rod-shaped, endospore forming, and motile. Growth occurred at 15-40 °C (optimum, 35 °C), pH 6.0-12.0 (optimum, 7.0) and in the presence of 1-20 % NaCl (optimum, 10 %). Strain FarD(T) used glucose, mannitol, melibiose, D-mannose, and 5 ketogluconate. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, and three phospholipids; MK-7 is the predominant menaquinone. The predominant cellular fatty acids were anteiso C15:0, anteiso C17:0, C20:0, and anteiso C19:0. The DNA G+C content was 42.1 mol%. Phylogenetic analysis of the small-subunit ribosomal RNA gene sequence indicated that strain FarD(T) had as its closest relative Virgibacillus salinus (similarity of 96.3 %). Based on phenotypic, phylogenetic, and taxonomic characteristics, strain FarD(T) is proposed as a novel species of the genus Virgibacillus within the order Clostridiales, for which the name V. natechei is proposed. The type strain is FarD(T) (=DSM 25609(T) = CCUG 62224(T)).

Identification of a New Serine Alkaline Peptidase from the Moderately Halophilic Virgibacillus natechei sp. nov., Strain FarDT and its Application as Bioadditive for Peptide Synthesis and Laundry Detergent Formulations

“…The type strain of Virgibacillus natechei is FarD T (DSM 25609 T or CCUG 62224 T ) [5]. As reported previously by the authors [5], strain FarD T was maintained at 35°C on Sehgal and Gibbons medium (SG) containing the following (in g/L): casamino acids, 7.5; yeast extract, 10; sodium glutamate, 1; trisodium citrate, 3; MgSO 4 ·7 H 2 O, 20; KCl, 2; FeSO 4 ·7 H 2 O, 0.036; and MnCl 2 ·4 H 2 O, 0.00036 supplemented with 100 g/L of NaCl at pH 7. After incubation at 35°C, a halo of casein degradation was revealed around the colony growth onto skimmed milk agar plates, as well described previously by the authors [17, 18].…”

“…The assessment of bacterial diversity of an Algerian saline lake revealed the presence of a novel specie of a genus Virgibacillus , namely Virgibacillus natechei sp. nov., strain FarD T with unusual phenotypic and genotypic characteristics [5]. In fact, the strain FarD T was mesophilic, moderately halophilic, and alkaliphilic.…”

“…The temperature range for growth was (15–40°C), with optimal growth occurring at 35°C. The pH range for growth was from 6 to 12, with an optimum at 7 [5]. No enzymatic research regarding this new species has been found in the literature, and for the first time with the current study, a research into the purification, characterization and biotechnological applicability of a new peptidase enzyme from strain FarD T was investigated.…”

Screening for Genes Coding for Putative Antitumor Compounds, Antimicrobial and Enzymatic Activities from Haloalkalitolerant and Haloalkaliphilic Bacteria Strains of Algerian Sahara Soils

“…However, it is also considered to be one of the less explored parts. Our team has been interested in these magnificent ecosystems for many years and the few studies that have been published have shown great active biomolecules [16–18], biodiversity of interesting new taxa [19–24], and enzymes [25, 26]. …”

Virgibacillus halotolerans sp. nov., isolated from a dairy product

“…At the time of writing, the genus Virgibacillus comprises 26 species with validly published names (http://www.bacterio.net/uw/virgibacillus. html); two other species, designated 'Virgibacillus zhanjiangensis' (Peng et al, 2009) and 'Virgibacillus natachei' (Amziane et al, 2013), have been proposed, but these names have not been validly published. Phylogenetically related genera are Oceanobacillus, Ornithinibacillus, Lentibacillus, Paucisalibacillus and Cerasibacillus.…”