Virion Polymerases

Bishop, David H. L.

doi:10.1007/978-1-4684-0832-4_3

Cited by 16 publications

(18 citation statements)

References 455 publications

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“…Animal viruses with virion-associated polymerase and ssRNA genomes are all enveloped and have negative-stranded RNA (Bishop, 1977). RSV has filamentous structures which resemble the structures of nucleocapsids isolated from enveloped arenavirus and bunyavirus particles (Bishop, 1977;Bishop & Shope, 1979; and RSV RNA translates poorly in vitro.…”

Section: Discussionmentioning

confidence: 99%

“…RSV has filamentous structures which resemble the structures of nucleocapsids isolated from enveloped arenavirus and bunyavirus particles (Bishop, 1977;Bishop & Shope, 1979; and RSV RNA translates poorly in vitro. These observations seem to suggest that RSV, which has an associated RNA-dependent RNA polymerase, might be a negative-strand virus.…”

Section: Discussionmentioning

confidence: 99%

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An RNA-dependent RNA Polymerase Associated with the Filamentous Nucleoproteins of Rice Stripe Virus

Toriyama

1986

Journal of General Virology

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SUMMARYFilamentous particles of rice stripe virus (RSV) were found to be associated with an RNA-dependent RNA polymerase activity. The enzyme catalysed the synthesis in vitro of single-and double-stranded RNAs corresponding in size with RNA found in RSV particles, as well as two other dsRNAs with mol. wt. of 1.0 × l06 and 0.56 × 106. The RSV polymerase activity required Mg 2+, Mn 2+ or Fe E+ but not Na +, K + or NH4 + which were inhibitory at concentrations of more than 50 mM. The optimum pH of the polymerase was around 8.0 at the optimum temperature, 40 °C. Detergent treatment of RSV particles did not enhance the polymerase activity. Purified RSV particles contained a minor polypeptide (mol. wt. 230000) as well as coat protein; the former may be the RSV polymerase. The taxonomy of RSV is discussed in relation to enveloped animal viruses with negative-stranded RNA, which also contain an RNAdependent RNA polymerase in virus particles.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

An RNA-dependent RNA Polymerase Associated with the Filamentous Nucleoproteins of Rice Stripe Virus

Toriyama

1986

Journal of General Virology

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“…Synthesis and purification of RNA. RNA was synthesized in vitro, using standard transcriptase reaction mixtures (2). Unlabeled SAM was used at a concentration of 0.8 mM.…”

Section: Methodsmentioning

confidence: 99%

“…Several eucaryotic and viral mRNA species have been shown to have a 5'-terminal cap structure with the sequence 7mGpppNm (21), the methylated cap structure being necessary for efficient translation (3). Sequence analyses of the in vitroor in vivo-synthesized 5' termini of the viral complementary mRNA species of the rhabdoviruses vesicular stomatitis virus (VSV) Indiana and VSV New Jersey have identified a variety of sequences, including both uncapped, capped, and capped and methylated species (1,2,4,7,10,11,15,16,18). The similarities between the 5' termini of the in vitro-synthesized RNA of the VSV serogroup viruses raises the question of whether such sequences have been conserved during the derivation of all the various serotypes and genera of the Rhabdoviridae family.…”

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confidence: 99%

5′-Terminal Sequences of Spring Viremia of Carp Virus RNA Synthesized In Vitro

Gupta

Bishop

Roy

1979

J Virol

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Sequence analyses have been undertaken on the 5′ termini of the RNA species synthesized in vitro at 22°C by Spring viremia of carp virion (SVCV)-associated transcriptase by using virus grown in mammalian BHK-21 cells. SVCV product RNA was synthesized in the absence or presence of low (0.56 μM) or high (0.8 mM) concentrations of added S -adenosyl- l -methionine (SAM). Two major sequences obtained in the absence (or in low concentrations) of SAM have been shown to be GpppAp and GpppAmpAp(C). A minor sequence detected when a low concentration of [ 3 H]SAM was added to reaction mixtures was 7mGpppAmpAp. Larger quantities of the 7mGpppAmpAp(C) sequence, in addition to the GpppAmpAp(C) sequence, were obtained when high concentrations of SAM were used, and under these conditions no GpppAp sequences were detected. It has further been shown that with low concentrations of [ 3 H]SAM the principle in vitro methylation of adenosine in SVCV product RNA occurred at the 2′- O -ribose position; no methylation at the N 6 -adenosine position and no internal product RNA methylation were detected. Comparison of the SVCV results to the published data on the 5′-terminal structures of the in vitro or in vivo mRNA species of vesicular stomatitis virus Indiana and vesicular stomatitis virus New Jersey suggests that the 5′ sequences of transcript RNA of different rhabdoviruses may have been conserved.

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“…Many eucaryotic viruses contain RNA polymerases that catalyze the synthesis offunctional viral mRNAs (2). In purified reoviruses (7) and vaccinia virus (14), additional activities are associated with the transcriptase complex.…”

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confidence: 99%

mRNA 5'-cap binding activity in purified influenza virus detected by simple, rapid assay

Kroath¹,

Shatkin²

1982

J Virol

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Reovirus mRNA 5'-terminal caps were 3'-radiolabeled with pCp and as affinity probes for proteins with cap binding activity. A rapid, simple, and sensitive blot assay was devised that could detect cellular cap binding protein in a complex polypeptide mixture. By using this method, cap binding activity was found in detergent-treated influenza virus but not in reovirus or vaccinia virus. Preincubation of capped reovirus mRNA with purified cellular cap binding protein reduced its primer effect on influenza transcriptase, whereas priming by ApG was not affected. The results indicate that influenza transcriptase complexes include cap-recognizing proteins that are involved in the formation of chimeric mRNAs.

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Virion Polymerases

Cited by 16 publications

References 455 publications

An RNA-dependent RNA Polymerase Associated with the Filamentous Nucleoproteins of Rice Stripe Virus

An RNA-dependent RNA Polymerase Associated with the Filamentous Nucleoproteins of Rice Stripe Virus

5′-Terminal Sequences of Spring Viremia of Carp Virus RNA Synthesized In Vitro

mRNA 5'-cap binding activity in purified influenza virus detected by simple, rapid assay

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