Virion RNA of Beet Yellows Closterovirus: Cell-free Translation and Some Properties

Karasev, Alexander V.; Agranovsky, A. A.; Рогов, В. В.; Miroshnichenko, N.A.; Dolja, V. V.; Atabekov, J.G.

doi:10.1099/0022-1317-70-1-241

Cited by 40 publications

(36 citation statements)

References 12 publications

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“…For example, antibody to the coat protein immunoprecipitated a large (approximately 200K) polypeptide in addition to the 26K coat protein product from in vitro translation products of CTV RNA (Lee & Calvert, 1987). Similarly, a large (250K) polypeptide was immunoprecipitated from translation products of RNA from beet yellows virus (BYV) by an antibody to BYV coat protein (Karasev et al, 1989). However, the results of the present study indicate that the CTV coat protein is not translated as part of a polyprotein.…”

Section: Discussioncontrasting

confidence: 55%

Molecular cloning and nucleotide sequencing of the coat protein gene of citrus tristeza virus

Sekiya

Lawrence

McCaffery

et al. 1991

Journal of General Virology

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Section: Discussioncontrasting

confidence: 55%

Molecular cloning and nucleotide sequencing of the coat protein gene of citrus tristeza virus

Sekiya

Lawrence

McCaffery

et al. 1991

Journal of General Virology

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“…RNA extracted from beet yellows virus (BYV) particles directed the synthesis of a major 250 kDa protein, covering only the Y-terminal half of the genome (Karasev et al, 1989). Seven other genes, including the CPG, are located downstream Brunstedt et al, 19913 and are most likely expressed only in planta from non-encapsidated sub-genomic RNAs Dolja et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

“…The viral CP was precipitated from the phenol phase using 2 vols of acetone and the concentration of CTV-CP was estimated by Coomassie blue staining of acrylamide gels and immunoblotting. The CTV-RNA was fractionated on a 5-20 % sucrose gradient as described by Karasev et al (1989).…”

Section: Methodsmentioning

confidence: 99%

Populations of citrus tristeza virus contain smaller-than-full-length particles which encapsidate sub-genomic RNA molecules

Mawassi

Gafny

Gagliardi

et al. 1995

Journal of General Virology

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Plants infected by citrus tristeza virus (CTV) contain, in addition to the 2000 nm full-length thread-like virions, a heterogeneous population of smaller particles. The CTV particles and RNA extracts from purified CTV preparations were fractionated by sucrose gradient centrifugation and the RNA molecules from different fractions translated in a reticulocyte translation system. Fractions containing predominantly an RNA band of approximately 3"2 kb directed the synthesis of CTV coat protein (CP), which in SDS-PAGE had an estimated molecular mass of 28 kDa. Three additional polypeptides, with estimated sizes of 21 kDa, 23 kDa and 27 kDa, were translated from a range of RNA molecules smaller than 3.2 kb. Hybridization with cDNA to the CP gene (CTV-CPG) and with a 350 base clone complementary to the 3' and 5' termini of the genes for CTV p20 and p23"5, respectively, indicated that preparations of CTV particles contain, in addition to the genomic (20 kb) RNA, two sub-genomic RNA molecules of 3-2 kb and 2-4kb and probably also two smaller molecules of 1.6 kb and 0.9 kb. Only the 3.2 kb RNA, its corresponding dsRNA molecule and populations of larger RNAs, including the 20kb genomic RNA, hybridized with a CTV-CPG probe, thus conflicting with our previous assignment of the CTV-CPG to the 0.8 kbp dsRNA. Based on these results we propose that distinct populations of CTV particles encapsidate smaller RNAs which were formed as a nested set of subgenomic RNAs. Sequence analysis of 2540 nucleotides downstream to CTV-CPG of strain VT revealed four open reading frames (ORFs) potentially encoding, in the 5' to 3' direction, 18 kDa (p18), 13 kDa (p13), 20 kDa (p20) and 23.5 kDa (p23.5) proteins. The CTV-VT ORFs showed variable but usually close levels of homology with the corresponding ORFs of CTV-T36 from Florida.

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“…4). It should be noted that the coat protein of beet yellows virus was not translatable from the 14.5 kb genomic ssRNA (Karasev et al, 1989). Assuming an average Mr of 110 per amino acid residue the 27K polypeptide corresponds to 92~ of the coding capacity of the 0.8 kbp segment.…”

mentioning

confidence: 99%

“…Compared with most other filamentous plant viruses (Hiebert & Dougherty, 1988) very little genomic characterization has been carried out on members of the closterovirus group (Bar-Joseph & Murant, 1982;Lee et al, 1988;Karasev et al, 1989). The main reason for this is the difficulty in purifying and isolating sufficient quantities of intact nucleic acid from viruses with large (14 to 19-5 kb) single-stranded genomic RNAs.…”

mentioning

confidence: 99%

In vitro translation of the citrus tristeza virus coat protein from a 0.8 kbp double-stranded RNA segment

Dulieu

Bar‐Joseph

1990

Journal of General Virology

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Virion RNA of Beet Yellows Closterovirus: Cell-free Translation and Some Properties

Cited by 40 publications

References 12 publications

Molecular cloning and nucleotide sequencing of the coat protein gene of citrus tristeza virus

Molecular cloning and nucleotide sequencing of the coat protein gene of citrus tristeza virus

Populations of citrus tristeza virus contain smaller-than-full-length particles which encapsidate sub-genomic RNA molecules

In vitro translation of the citrus tristeza virus coat protein from a 0.8 kbp double-stranded RNA segment

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