Virological Analysis of a Regional Mumps Outbreak in the Northern Island of Japan&amp;mdash;Mumps Virus Genotyping and Clinical Description

Okajima, Kazuki; Iseki, Kenichi; Koyano, Shin; Kato, Atsushi; Azuma, Hiroshi

doi:10.7883/yoken.66.561

Cited by 8 publications

(2 citation statements)

References 25 publications

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“…Mumps is a vaccine-preventable disease, and worldwide vaccination campaigns have led to a considerable reduction of incidence (3)(4)(5). However, in recent years, an increasing number of mumps outbreaks have been reported worldwide within vaccinated populations (6)(7)(8)(9)(10)(11).…”

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confidence: 99%

Mumps Virus SH Protein Inhibits NF-κB Activation by Interacting with Tumor Necrosis Factor Receptor 1, Interleukin-1 Receptor 1, and Toll-Like Receptor 3 Complexes

Franz

Rennert

Woznik

et al. 2017

J Virol

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The mumps virus (MuV) small hydrophobic protein (SH) is a type I membrane protein expressed in infected cells. SH has been reported to interfere with innate immunity by inhibiting tumor necrosis factor alpha (TNF-␣)-mediated apoptosis and NF-B activation. To elucidate the underlying mechanism, we generated recombinant MuVs (rMuVs) expressing the SH protein with an N-terminal FLAG epitope or lacking SH expression due to the insertion of three stop codons into the SH gene. Using these viruses, we were able to show that SH reduces the phosphorylation of IKK␤, IB␣, and p65 as well as the translocation of p65 into the nucleus of infected A549 cells. Reporter gene assays revealed that SH interferes not only with TNF-␣-mediated NF-B activation but also with IL-1␤-and poly(I·C)-mediated NF-B activation, and that this inhibition occurs upstream of the NF-B pathway components TRAF2, TRAF6, and TAK1. Since SH coimmunoprecipitated with tumor necrosis factor receptor 1 (TNFR1), RIP1, and IRAK1, we hypothesize that SH exerts its inhibitory function by interacting with TNFR1, interleukin-1 receptor type 1 (IL-1R1), and TLR3 complexes in the plasma membrane of infected cells.IMPORTANCE The MuV SH has been shown to impede TNF-␣-mediated NF-B activation and is therefore thought to contribute to viral immune evasion. However, the mechanisms by which SH mediates NF-B inhibition remained largely unknown. In this study, we show that SH interacts with TNFR1, IL-1R1, and TLR3 complexes in infected cells. We thereby not only shed light on the mechanisms of SH-mediated NF-B inhibition but also reveal that SH interferes with NF-B activation induced by interleukin-1␤ (IL-1␤) and double-stranded RNA.

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confidence: 99%

Mumps Virus SH Protein Inhibits NF-κB Activation by Interacting with Tumor Necrosis Factor Receptor 1, Interleukin-1 Receptor 1, and Toll-Like Receptor 3 Complexes

Franz

Rennert

Woznik

et al. 2017

J Virol

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“…When the amount of the viral genome in clinical specimens is under the detectable level, virus cultivation in culture cells (Vero cells or Caco-2 cells being used) is needed. Although mumps is preventable with a vaccine, large outbreaks and re-emergence of mumps have occurred in vaccinated populations including college students in the UK, Japan, and USA [ 1 , 4 ].…”

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confidence: 99%

Easy and Rapid Detection of Mumps Virus by Live Fluorescent Visualization of Virus-Infected Cells

et al. 2015

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Mumps viruses show diverse cytopathic effects (CPEs) of infected cells and viral plaque formation (no CPE or no plaque formation in some cases) depending on the viral strain, highlighting the difficulty in mumps laboratory studies. In our previous study, a new sialidase substrate, 2-(benzothiazol-2-yl)-4-bromophenyl 5-acetamido-3,5-dideoxy-α-D-glycero-D-galacto-2-nonulopyranosidonic acid (BTP3-Neu5Ac), was developed for visualization of sialidase activity. BTP3-Neu5Ac can easily and rapidly perform histochemical fluorescent visualization of influenza viruses and virus-infected cells without an antiviral antibody and cell fixation. In the present study, the potential utility of BTP3-Neu5Ac for rapid detection of mumps virus was demonstrated. BTP3-Neu5Ac could visualize dot-blotted mumps virus, virus-infected cells, and plaques (plaques should be called focuses due to staining of infected cells in this study), even if a CPE was not observed. Furthermore, virus cultivation was possible by direct pick-up from a fluorescent focus. In conventional methods, visible appearance of the CPE and focuses often requires more than 6 days after infection, but the new method with BTP3-Neu5Ac clearly visualized infected cells after 2 days and focuses after 4 days. The BTP3-Neu5Ac assay is a precise, easy, and rapid assay for confirmation and titration of mumps virus.

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The antiviral effect of catechins on mumps virus infection

Takahashi

Kurebayashi

Tani

et al. 2021

Journal of Functional Foods

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Virological Analysis of a Regional Mumps Outbreak in the Northern Island of Japan—Mumps Virus Genotyping and Clinical Description

Cited by 8 publications

References 25 publications

Mumps Virus SH Protein Inhibits NF-κB Activation by Interacting with Tumor Necrosis Factor Receptor 1, Interleukin-1 Receptor 1, and Toll-Like Receptor 3 Complexes

Mumps Virus SH Protein Inhibits NF-κB Activation by Interacting with Tumor Necrosis Factor Receptor 1, Interleukin-1 Receptor 1, and Toll-Like Receptor 3 Complexes

Easy and Rapid Detection of Mumps Virus by Live Fluorescent Visualization of Virus-Infected Cells

The antiviral effect of catechins on mumps virus infection

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