Virome analysis for identification of viruses associated with asymptomatic infection of purple passion fruit (<i>Passiflora edulis</i> f. edulis) in Colombia

Sepúlveda, Mariana; Cardona, Daniela; García, Yuliana Gallo; Higuita, Mónica; Gutiérrez, Pablo

doi:10.1080/14620316.2021.1973583

Cited by 6 publications

(6 citation statements)

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“…The advancement of virome databases, powered by reliable and precise HTS techniques, has transformed virology and created new avenues for research opportunities. These include managing disease risks [ 54 ], tracking emerging diseases, and implementing new phytosanitary regulations [ 55 ]. Hence, HTS offers a significant advantage for virome characterization over conventional techniques such as ELISA, microarray, or (RT-) PCR detection because its efficacy is primarily dictated by the completeness of the reference databases [ 56 ], the depth of the data [ 57 ], and the accuracy of the base calls [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

Deciphering the virome of Chunkung (Cnidium officinale) showing dwarfism-like symptoms via a high-throughput sequencing analysis

Belete,

Kim,

Gudeta

et al. 2024

Virol J

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Background Viruses have notable effects on agroecosystems, wherein they can adversely affect plant health and cause problems (e.g., increased biosecurity risks and economic losses). However, our knowledge of their diversity and interactions with specific host plants in ecosystems remains limited. To enhance our understanding of the roles that viruses play in agroecosystems, comprehensive analyses of the viromes of a wide range of plants are essential. High-throughput sequencing (HTS) techniques are useful for conducting impartial and unbiased investigations of plant viromes, ultimately forming a basis for generating further biological and ecological insights. This study was conducted to thoroughly characterize the viral community dynamics in individual plants. Results An HTS-based virome analysis in conjunction with proximity sampling and a tripartite network analysis were performed to investigate the viral diversity in chunkung (Cnidium officinale) plants. We identified 61 distinct chunkung plant-associated viruses (27 DNA and 34 RNA viruses) from 21 known genera and 6 unclassified genera in 14 known viral families. Notably, 12 persistent viruses (7 DNA and 5 RNA viruses) were exclusive to dwarfed chunkung plants. The detection of viruses from the families Partitiviridae, Picobirnaviridae, and Spinareoviridae only in the dwarfed plants suggested that they may contribute to the observed dwarfism. The co-infection of chunkung by multiple viruses is indicative of a dynamic and interactive viral ecosystem with significant sequence variability and evidence of recombination. Conclusions We revealed the viral community involved in chunkung. Our findings suggest that chunkung serves as a significant reservoir for a variety of plant viruses. Moreover, the co-infection rate of individual plants was unexpectedly high. Future research will need to elucidate the mechanisms enabling several dozen viruses to co-exist in chunkung. Nevertheless, the important insights into the chunkung virome generated in this study may be relevant to developing effective plant viral disease management and control strategies.

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Section: Discussionmentioning

confidence: 99%

Deciphering the virome of Chunkung (Cnidium officinale) showing dwarfism-like symptoms via a high-throughput sequencing analysis

Belete,

Kim,

Gudeta

et al. 2024

Virol J

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“…2 especially due to diseases caused by viral (Sepúlveda et al, 2022), bacterial or fungal etiologies (Joy and Sherin, 2016). Among these microorganisms Xanthomonas bacteria and Fusarium fungus are frequently involved (Fernández Barbosa and Suarez Meza, 2009;Benítez et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of Bothrops asper and Porthidium nasutum venom on purple passion fruit (Passiflora edulis f. edulis) phytopathogens

Núñez-Rangel,

Rey-Suárez,

Serna

et al. 2023

Rev. Colomb. Cienc. Hortic

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The most important pathogens causing high damage to fruit and vegetable crops are bacteria and fungi; among them Xanthomonas and Fusarium respectively are in the phytopathogens responsible of diseases in those crops. In Colombia, the crop of Passiflora edulis f. edulis occupies a position preferential within international market. However different pathologies affect their production, one of them is the infectious diseases and are caused by microorganisms as Xanthomonas and Fusarium. The control includes application of pesticides or antibiotics, but to some of them the microorganisms have develop resistance is widespread, and others are restricted associated to the impact on the environment and effects in animal and human healthy. Then find other alternatives is necessary, in this work the snake venoms of the vipers Bothrops asper and Porthidium nasutum were evaluated in ability to inhibit growth of these microorganism isolated of purple passion fruit crops with typical symptoms of diseases associated with infections by microorganisms. For these antimicrobial assays were performed in agar culture and broth microdilution. The results showed growth inhibition of 80% to B. asper against Xanthomonas axonopodis and 70% Xanthomonas perforans. The minimum inhibitory concentration was 31,2 µg mL-1 to B. asper and 500 µg mL-1 to P. nasutum to both bacteria. Nevertheless, the activity against F. oxysporum was low. The isolation of compounds responsible of this activity will contribute to the development of new molecules for antimicrobial agents against phytopathogens affecting purple passion fruit crops.

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“…Unfortunately, the productivity of purple passion fruit in Colombia started to be seriously compromised by the rapid emergence of different viral diseases that remained uncharacterized until recent years (Jaramillo et al, 2018(Jaramillo et al, , 2019Sepúlveda et al, 2021). To address this problem, a series of high throughput sequencing (HTS) surveys have been performed in the province of Antioquia.…”

Section: Introductionmentioning

confidence: 99%

“…To address this problem, a series of high throughput sequencing (HTS) surveys have been performed in the province of Antioquia. These studies have revealed that the most prevalent viruses infecting purple passion fruit in the region are the potyvirus soybean mosaic virus (SMV), the tymovirus passion fruit yellow mosaic virus (PFYMV), the cucumovirus cucumber mosaic virus (CMV) and a new badnavirus tentatively named gulupa bacilliform virus A (GBVA) (tentative binomial name: Badnavirus passiflorae) (Jaramillo et al, 2018(Jaramillo et al, , 2019Sepúlveda et al, 2021). In addition, serological and PCR-based methods suggest the circulation of the potyvirus cowpea aphid-borne mosaic virus (CABMV) in the province of Cundinamarca (Camelo, 2010;Gordillo, 2011).…”

Section: Introductionmentioning

confidence: 99%

Natural infection of purple passion fruit (Passiflora edulis f. edulis) by a novel member of the family Tymoviridae in Colombia

Cardona¹,

Restrepo²,

Higuita³

et al. 2022

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Purple passion fruit is one of the most important fruit exports of Colombia, but its productivity is being compromised by the emergence of several viral diseases. High-throughput sequencing (HTS) surveys of viruses in purple passion fruit fields in the province of Antioquia suggested infection by a new member of the family Tymoviridae. In this work, we characterize the complete genome sequence of this virus, tentatively named purple passionfruit leaf deformation virus (PpLDV), and evaluate its distribution in Antioquia. PpLDV was assembled at high coverage in four datasets from different regions. The 6.1 kb genome of PpLDV encodes a single polyprotein with domains characteristic of the family Tymoviridae, contains a marafibox-like promoter and the 3'-UTR can fold into a tRNA-like secondary structure with a valine anti-codon. Phylogenetic analysis of the polyprotein revealed that PpLDV is a distinct member of the family Tymoviridae, more closely related to the genus Tymovirus and the unclassified Poinsettia mosaic virus (PnMV). The presence of PpLDV was confirmed by RT-qPCR and RT-PCR in samples from commercial purple passion fruit fields, plantlets and seed sprouts collected in Antioquia using primers designed in this study.

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Virome analysis for identification of viruses associated with asymptomatic infection of purple passion fruit (Passiflora edulis f. edulis) in Colombia

Cited by 6 publications

References 50 publications

Deciphering the virome of Chunkung (Cnidium officinale) showing dwarfism-like symptoms via a high-throughput sequencing analysis

Deciphering the virome of Chunkung (Cnidium officinale) showing dwarfism-like symptoms via a high-throughput sequencing analysis

Antimicrobial activity of Bothrops asper and Porthidium nasutum venom on purple passion fruit (Passiflora edulis f. edulis) phytopathogens

Natural infection of purple passion fruit (Passiflora edulis f. edulis) by a novel member of the family Tymoviridae in Colombia

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