Virosomes: Cationic Liposomes Enhance Retroviral Transduction

Hodgson, Clague P.; Solaiman, Fauzia

doi:10.1038/nbt0396-339

Cited by 85 publications

(63 citation statements)

References 15 publications

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“…Polycationic agents such as polybrene and DEAE dextran are often used to increase the transduction efficiency in mammalian cells (Hodgson et al, 1996;Singh and Rigby, 1996). In the absence of polycationic agents, virus and cells tend to repel each other as they both have negatively charged surfaces.…”

Section: Discussionmentioning

confidence: 99%

Efficient Enhancement of Lentiviral Transduction Efficiency in Murine Spermatogonial Stem Cells

Kim

et al. 2012

Molecules and Cells

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Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis throughout postnatal life in male and have the ability to transmit genetic information to the subsequent generation. In this study, we have optimized the transduction efficiency of SSCs using a lentiviral vector by considering different multiplicity of infection (MOI), duration of infection, presence or absence of feeder layer and polycationic agents. We tested MOI of 5, 10 or 20 and infection duration of 6, 9 or 12 h respectively. After infection, cells were cultured for 1 week and as a result, the number of transduced SSCs increased significantly for MOI of 5 and 10 with 6 h of infection. When the same condition (MOI of 5 with 6 hours) was applied in presence or absence of STO feeder layer and infected SSCs were cultured for 3 weeks on the STO feeder layer, a significant increase in the number of transduced cells was observed for without the feeder layer during infection. We subsequently studied the effects of polycationic agents, polybrene and dioctadecylamidoglycyl spermine (DOGS), on the transduction efficiency. Compared with the polybrene treatment, the recovery rate of the transduced SSCs was significantly higher for the DOGS treatment. Therefore, our optimization study could contribute to the enhancement of germ-line modification of SSCs using lentiviral vectors and in generation of transgenic animals.

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Section: Discussionmentioning

confidence: 99%

Efficient Enhancement of Lentiviral Transduction Efficiency in Murine Spermatogonial Stem Cells

Kim

et al. 2012

Molecules and Cells

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“…Polybrene (14), DEAE-dextran (15), or poloxamers (16)), cationic lipids (e.g. Lipofectin or Lipofectamine (17,18)), or cationic peptides/proteins (e.g. fibronectin fragments (19 -21), protamine sulfate (22), prostatic acid phosphatase fragments (23), or HIV-1 gp41-and gp120-derived peptides (24,25)).…”

Section: Lentiviral Vectors (Lvs)mentioning

confidence: 99%

Molecular Determinants of Vectofusin-1 and Its Derivatives for the Enhancement of Lentivirally Mediated Gene Transfer into Hematopoietic Stem/Progenitor Cells

Majdoul

Seye

Kichler

et al. 2016

Journal of Biological Chemistry

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Gene delivery into hCD34؉ hematopoietic stem/progenitor cells (HSPCs) using human immunodeficiency virus, type 1-derived lentiviral vectors (LVs) has several promising therapeutic applications. Numerous clinical trials are currently underway. However, the efficiency, safety, and cost of LV gene therapy could be ameliorated by enhancing target cell transduction levels and reducing the amount of LV used on the cells. Several transduction enhancers already exist, such as fibronectin fragments or cationic compounds. Recently, we discovered Vectofusin-1, a new transduction enhancer, also called LAH4-A4, a short histidine-rich amphipathic peptide derived from the LAH4 family of DNA transfection agents. Vectofusin-1 enhances the infectivity of lentiviral and ␥-retroviral vectors pseudotyped with various envelope glycoproteins. In this study, we compared a family of Vectofusin-1 isomers and showed that Vectofusin-1 remains the lead peptide for HSPC transduction enhancement with LVs pseudotyped with vesicular stomatitis virus glycoproteins and also with modified gibbon ape leukemia virus glycoproteins. By comparing the capacity of numerous Vectofusin-1 variants to promote the modified gibbon ape leukemia virus glycoprotein-pseudotyped lentiviral vector infectivity of HSPCs, the lysine residues on the N-terminal extremity of Vectofusin-1, a hydrophilic angle of 140°formed by the histidine residues in the Schiffer-Edmundson helical wheel representation, hydrophobic residues consisting of leucine were all found to be essential and helped to define a minimal active sequence. The data also show that the critical determinants necessary for lentiviral transduction enhancement are partially different from those necessary for efficient antibiotic or DNA transfection activity of LAH4 derivatives. In conclusion, these results help to decipher the action mechanism of Vectofusin-1 in the context of hCD34؉ cell-based gene therapy.

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“…Recently several authors have demonstrated in vitro that complexing virus particles with cationic amphiphiles can enhance virus infection above that observed in the presence of polybrene. [11][12][13] Such amphiphiles have been used for several years to mediate DNA transfer to cells, albeit at a lower efficiency than viruses. They include cationic liposomes which are lamellar vesicles and a variety of cationic compounds in either linear or branched form with the ability to complex negatively charged molecules.…”

Section: Introductionmentioning

confidence: 99%

Enhanced in vitro and in vivo gene delivery using cationic agent complexed retrovirus vectors

et al. 1998

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Retroviruses are, at present, the most efficient integrative mediated infection without cytotoxicity. To increase virus vectors available for gene delivery. However, these viruses infectivity further we combined the enhancing effect of are still limited by relatively low titres. Although several pro-DOGS on virus infectivity with concentration of virus partocols exist to improve virus titre most of them are timeticles by ultrafiltration to reach titres of 1 × 10 9 IU/ml. The consuming and unable to provide sufficient virus for in vivo in vivo transduction of regenerating rat liver, by an amphoapplications. Virus titre can be enhanced by polybrene and tropic retrovirus was increased approximately five-fold by other cationic agents. By investigating a broad range of the addition of DOGS compared with virus alone. There cationic agents for their ability to enhance virus infectivity was no animal toxicity observed following the adminiswe found that both ecotropic and amphotropic retrovirus tration of DOGS. The improved transduction efficiency infection could be increased. In particular, the lipopolyseen both in vitro and in vivo following the co-adminisamine dioctadecylamidoglycylspermine (DOGS) gave up to tration of DOGS/virus complexes may be useful for future one order of magnitude enhancement above polybrenegene therapy applications.

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Virosomes: Cationic Liposomes Enhance Retroviral Transduction

Cited by 85 publications

References 15 publications

Efficient Enhancement of Lentiviral Transduction Efficiency in Murine Spermatogonial Stem Cells

Efficient Enhancement of Lentiviral Transduction Efficiency in Murine Spermatogonial Stem Cells

Molecular Determinants of Vectofusin-1 and Its Derivatives for the Enhancement of Lentivirally Mediated Gene Transfer into Hematopoietic Stem/Progenitor Cells

Enhanced in vitro and in vivo gene delivery using cationic agent complexed retrovirus vectors

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