-The purpose of this study is to investigate users' wayfinding task performance and subjective preference in a three-dimensional (3D) virtual environment. The research variables are interaction mode (touch sensitive vs body movement modes), viewing perspective [first-person perspective (1PP) versus third-person perspective (3PP)], and gender (man versus woman). Three difficult levels of tasks were conducted, i.e., A, A-P, and A-A types. The experiment is 2 × 2 × 2 between-subject design. Participants needed to complete three wayfinding tasks and fill out subjective preference questionnaires regarding task fluency, satisfaction, system usability scale, and NASA Task Load Index (NASA-TLX). Fifty-six participants (28 men and 28 women) were invited using convenient sampling method. The results are as follows: (1) Participants adopting touch-sensitive mode performed better than body movement mode. (2) Participants using touch-sensitive mode could have better task fluency and higher SUS score, and with less effort and frustration. (3) Participants adopting 3PP had better wayfinding task performance than first-person perspective. They also had higher system usability scale score and spent less effort for having wider viewing perspective. (4) The gender variable only showed significant effect in A-P type of wayfinding task. Men performed better than women. It is concluded that adopting touch-sensitive interaction and 3PP designs can better facilitate users' wayfinding tasks.