Virulence and antimicrobial resistance factors in Salmonella enterica serotypes isolated from pigs and chickens in central Chile

Abstract

Salmonella enterica is a food-borne pathogen with a wide host-range that during decades has been of public health concern in developed and developing countries. In Chile, the poultry and pig industries represent the biggest contribution of meat consumption in the population, and sanitary regulations have been imposed for Salmonella control. The aim of this work was to determine and characterize Salmonella strains isolated from pigs and chicken raised on commercials farms in Chile. For this, isolates belonging to pigs (n = 46) and poultry (n = 57) were genotyped by two multiplex PCR reactions and virulotyped by the PCR detection of virulence-associated genes. In addition, isolates were serotyped and analyzed by the Kirby Bauer assay to determine their antimicrobial resistance phenotypes. From these analyses 52 genotypes, six serotypes and several multidrug resistance phenotypes and different combinations of virulence-associated genes were detected. These results suggest that S. enterica in pigs and poultry in central Chile should be monitored due to potential consequences in public and animal health.

Epidemiological Characterization of Isolates of Salmonella enterica and Shiga Toxin-Producing Escherichia coli from Backyard Production System Animals in the Valparaíso and Metropolitana Regions

“…In this study, five strains of S. enterica were isolated, with five different antimicrobial resistance profiles, 80% of them being MDR. Among the groups of antimicrobials to which resistance was reported were Betalactams, Tetracyclines, Nitrofurans, and Phenicols; similar results were reported for different animal systems from central Chile [43], which includes S. enterica isolates from BPS, also observed in isolates from industrial pigs and chickens in Chile [85]. Within the Betalactams, resistance was reported against Penicillin, firstand third-generation Cephalosporins, and Carbapenemics, all of which are considered critically important in human medicine based on five prioritization criteria according to the World Health Organization (WHO) [86].…”

Cell membrane-coated nanoparticles: An emerging antibacterial platform for pathogens of food animals

“…It is a foodborne pathogen, possess several zoonotic serotypes as well as a wide range of host organisms including poultry, ovine, bovine, and humans ( 111 ). It is the most common type of infectious agent, causing animal and human Salmonellosis ( 112 , 113 ).…”