Virulence and Cultural Characteristics of Two<i>Aspergillus flavus</i>Strains Pathogenic on Cotton

Cotty, Peter J.

doi:10.1094/phyto-79-808

Cited by 395 publications

(351 citation statements)

References 17 publications

Supporting

Mentioning

340

Contrasting

Unclassified

Order By: Relevance

“…a Strain classification of A. flavus isolate [7]. b Reaction type derived from pectinase culture assay: CZ = clear zone; RR = red ring.…”

Section: Resultsmentioning

confidence: 99%

“…Soil samples were collected in fields planted in cotton in a period from April to May of a given year. The isolates tested in Survey 1 (Table 1) were obtained in an earlier study [7]. The isolates tested in Survey 2 were obtained in a second soil sampling done in 1993 [9].…”

Section: Biological Materialsmentioning

confidence: 99%

“…Propagule suspensions were then spread on modified rose Bengal medium. Isolation plates were incubated for 3 days at 31 • C, after which discrete colonies were transferred to 5/2 agar (5% V-8 vegetable juice, 2% agar) [7]. Isolates were maintained on 5/2 agar at 30 • C or on agar plugs submerged in sterile deionized water [7].…”

Section: Biological Materialsmentioning

confidence: 99%

“…Isolation plates were incubated for 3 days at 31 • C, after which discrete colonies were transferred to 5/2 agar (5% V-8 vegetable juice, 2% agar) [7]. Isolates were maintained on 5/2 agar at 30 • C or on agar plugs submerged in sterile deionized water [7]. Conidial suspensions in sterile deionized water (10 5 to 10 6 spores/ml) were used for inoculations.…”

Section: Biological Materialsmentioning

confidence: 99%

“…This collection of isolates consisted of two physiologically and morphologically distinct strains, S and L [7]. Strain S isolates produce abundant small sclerotia (<400 µm in diameter) and high levels of aflatoxin, while strain L isolates produce fewer but larger sclerotia (>400 µm in diameter), on average, and lower aflatoxin levels.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Expression of Pectinase Activity Among Aspergillus Flavus Isolates from Southwestern and Southeastern United States

Mellon

Cotty

2004

Mycopathologia

View full text Add to dashboard Cite

Aspergillus flavus is a widely distributed filamentous fungus that contaminates crops with the potent carcinogen aflatoxin. This species can be divided into S and L strains on the basis of sclerotial morphology. During crop infection, A. flavus can secrete a large array of hydrolytic enzymes. These include pectinase, which aids fungal spread through plant tissues. A survey of pectinase expression by soil isolates derived from different regions of the United States revealed geographic polymorphisms. Strain L isolates from Arizona produced moderate to high levels of a specific pectinase P2c, while S strain isolates produced variable amounts of P2c. In contrast, L strain isolates from southeastern U.S. yielded variable P2c production, while S strain isolates consistently expressed high P2c levels. These results were corroborated by pectinase surveys of additional collections of A. flavus from soil and cottonseed. Expression patterns for P2c and pectinmethylesterase were evaluated for a select number of isolates using an isoelectric focusing technique. Clear zone reactions from the pectinase plate assay corresponded to the presence of P2c, while red ring reactions corresponded to the lack of P2c. Commercial cottonseed infected by S strain isolates frequently contained aflatoxin, even when infected by S strain isolates that did not produce pectinase P2c. Thus, although P2c-lacking isolates have reduced invasiveness, these isolates still have sufficient pathogenicity to cause aflatoxin contamination.

show abstract

“…a Strain classification of A. flavus isolate [7]. b Reaction type derived from pectinase culture assay: CZ = clear zone; RR = red ring.…”

Section: Resultsmentioning

confidence: 99%

Section: Biological Materialsmentioning

confidence: 99%