Virulence and cytotoxicity of seafood borne Aeromonas hydrophila

Illanchezian, Seethalakshmi; Jayaraman, Sathishkumar; Manoharan, Muthu Saravanan; Valsalam, Saritha

doi:10.1590/s1517-83822010000400016

Cited by 27 publications

(13 citation statements)

References 18 publications

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“…The prevalence of cytolytic enterotoxin gene carrying A. hydrophila isolates reported in our study are comparable with those reported from Malaysia [9,13] . However, our results are higher than the results of a study conducted in India [27] .…”

Section: Discussionsupporting

confidence: 89%

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Occurrence of Aeromonas hydrophila in Fish, Shrimp, Lobster and Crab in Iran

Rahimi¹,

Raissy²,

Razzaghimanesh³

et al. 2014

Kafkas Univ Vet Fak Derg

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SummaryThis study was conducted to determine the prevalence rate and antimicrobial resistance of Aeromonas hydrophila in fish, shrimp, lobster and crab caught off the south coast of Iran. A total of 541 samples including, 133 freshly caught fish of 4 different types including Otollithes ruber, Pamous argenteus, Parastromateus niger and Psettodes erumel, 240 shrimp of 4 different species including Penaeus monodon, P. semisulcatus, P. indicus, and P. merguiensis, 108 lobster (Panulirus homarus) and 60 crab (Panulirus homarus) were collected in 3 provinces along Persian Gulf in the south coast of Iran. Samples were collected at the end of each month from September 2011 to May 2012. Using conventional bacteriological techniques, 66 A. hydrophila isolates were identified in which 62 strains were confirmed by PCR assay targeting 16S rDNA gene of A. hydrophila. Using PCR assays targeting the A. hydrophila cytolytic enterotoxin gene, 57 (10.5%) isolates were positive. The highest prevalence of A. hydrophila was found in fish (19.5%), followed by shrimp (9.2%), lobster (9.3%) and crab (6.7%). The highest prevalence of A. hydrophila occurred in summer (21.3%) followed by fall (12.0%), spring (10.8%), and winter (5.6%). To our knowledge, the present study is the first report of the isolation of A. hydrophila from fish, shrimp, lobster and crab in Iran. A. hydrophila sitotoksik enterotoksin gen hedefi ile uygulanan PCR metodu ile 57 (%10.5) izolat pozitif olarak tespit edilmiştir. A. hydrophila prevalansı en yüksek olarak balıklarda (%19.5), sonrasında ise sırasıyla ıstakozlarda (%9.3), karideslerde (%9,2) ve yengeçlerde (%6.7) belirlenmiştir. Mevsimsel olarak en yüksek A. hydrophila prevalansı yaz ayında (%21.3) olurken bunu sırasıyla sonbahar (%12.0), ilkbahar (%10.8) ve kış (%5.6) izledi. Bizim bilgimiz kapsamında bu çalışma İran'da balık, karides, ıstakoz ve yengeçlerde A. hydrophila izolasyonunun rapor edildiği ilk çalışmadır. Keywords

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Section: Discussionsupporting

confidence: 89%

“…Also several investigations have shown that members of the genus Aeromonas are also widely distributed in various foods such as meat [9,10] , sea food [11][12][13][14] , and vegetable [15] . Thus, foods have been suggested as a vector in the dissemination of this pathogen.…”

Section: Introductionmentioning

confidence: 99%

Occurrence of Aeromonas hydrophila in Fish, Shrimp, Lobster and Crab in Iran

Rahimi¹,

Raissy²,

Razzaghimanesh³

et al. 2014

Kafkas Univ Vet Fak Derg

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show abstract

“…Estudos demonstram que A. hydrophila possui vários fatores de virulência, citotoxinas e enzimas extracelulares com atividade proteolítica e hemolítica, importantes para o desenvolvimento da patogênese. Produzem também substâncias glicoconjugadas que auxiliam na formação de biofilme 10 , o qual impede a ação dos medicamentos no tecido lesionado, levando à falha no tratamento antimicrobiano 4,11 .…”

Section: Discussionunclassified

Fasciite necrosante por Aeromonas hydrophila e Staphylococcus epidermidis – relato de caso

et al. 2016

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Introdução: Fasciite necrosante (FN) é uma infecção rara dos tecidos subcutâneos e fáscia superficial, geralmente confundida com infecção benigna. Entretanto, apresenta enorme potencial para o desenvolvimento de complicações graves que contribuem para os elevados índices de mortalidade. Objetivos: Descrever um caso de FN polimicrobiana ocasionada por Aeromonas hydrophila e Staphylococcus epidermidis em paciente portador de síndrome da imunodeficiência adquirida, hepatite C e diabetes mellitus. Métodos: Analisaram-se dados de prontuário e resultados de exames laboratoriais de paciente internado no Hospital Universitário de Santa Maria, Santa Maria, Rio Grande do Sul. Resultados: Paciente do sexo masculino, 47 anos, com relato de fratura exposta em membro inferior esquerdo, desenvolvendo infecção no ferimento. Após desbridamento de tecido desvitalizado, identificaram-se A. hydrophila e S. epidermidis. Paciente continua em tratamento e aguarda cirurgia para enxerto. Conclusões: A FN é uma enfermidade rara que merece toda a atenção médica, pois a identificação e tratamento precoces são fundamentais para a recuperação física do paciente.

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“…It is pathogenic to shrimps, frogs, and fishes (Martin-Carnahan and Joseph, 2005;EPA, 2006). The infection caused by this bacterium can lead to significant mortality rates within a short period, causing losses to fish and shrimp breeders (Illanchezian et al, 2010;Mangunwardoyo et al, 2010). The disease symptoms vary, but infected fish generally show skin ulcers and haemorrhaging in the skin, gills, and oral cavity (Gardenia et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of identification techniques for the fish pathogen, Aeromonas hydrophila, from Indonesia

Kusumawaty¹,

Pancoro²,

Aryantha³

et al. 2016

MJM

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Aims:This study evaluated the accuracy of three methods used in the identification of Aeromonas hydrophila, a Gramnegative bacterium found in warm aquatic environments. A. hydrophila samples from Indonesia were tested using (a) SNI 7303, developed by the Indonesian government, (b) the method of Dorsch and (c) the method of Cascón. The results obtained were compared to that of the gold standard method, which used 16S rDNA sequences. Methodology and results: Based on the Indonesian government standard identification method SNI7303, we identified 56 out of 95 samples as A. hydrophila. The samples were then screened using the PCR amplification approach developed by Dorsch and Cascón. Of the 56 samples, only 20 samples were found to be positive by either the Dorsch or Cascón methods. DNA from these 20 samples was amplified using common 16S rDNA primers and the sequences compared with available 16S rDNA sequences from the GenBank. Phylogenetic analyses on the data were performed using Clustal X and MEGA 5 software. Conclusion, significance and impact of study: Of the 56 samples positively identified as A. hydrophila using the BSN method, identity in only five samples were positively confirmed using the16S rDNA method, giving an accuracy of only 8.9%. In this connection, the Dorsch method was 31.3% accurate while the Cascón method provided 45.5% correct identification. When all three methods were used in combination, 71.4% of the samples were correctly identified. The results of the study show that methods used to identify A. hydrophila cannot be used with confidence to identify A. hydrophila from Indonesia and probably from other tropical regions as well. The genetic diversity of Aeromonas bacteria in Indonesia appears to be considerably higher than that encountered by Dorsch or Cascón. Therefore, there is a need to develop a new simple method to identify A. hydrophila from tropical regions.

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Virulence and cytotoxicity of seafood borne Aeromonas hydrophila

Cited by 27 publications

References 18 publications

Occurrence of Aeromonas hydrophila in Fish, Shrimp, Lobster and Crab in Iran

Occurrence of Aeromonas hydrophila in Fish, Shrimp, Lobster and Crab in Iran

Fasciite necrosante por Aeromonas hydrophila e Staphylococcus epidermidis – relato de caso

Evaluation of identification techniques for the fish pathogen, Aeromonas hydrophila, from Indonesia

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