Virulence and resistance determinants in Pseudomonas aeruginosa isolated from pericarditis in diseased broiler chickens in Egypt

Abstract

Objective: This study was performed to probe the antimicrobial resistance and virulence genes profiling in Pseudomonas aeruginosa recovered from the cases of pericarditis in broiler chickens. Materials and methods: The samples ( n = 250) collected from the cases of pericarditis in broiler chickens were bacteriologically examined. Antimicrobial susceptibility was tested by disc diffusion technique. The isolates were genotypically studied for the presence of antimicrobial resistance and virulence gene traits. Finally, the nucleotide sequence of representative resistance gene ( mexR gene) and virulence genes ( tox A and las I genes) was analyzed. Results: P. aeruginosa was isolated from 45 samples (18%). Antimicrobial susceptibility testing revealed multidrug resistance in most of the recovered P. aeruginosa isolates, whereas colistin and imipenem were the furthermost in vitro-sensitive antibiotics. Antimicrobial resistance genes, such as bla CTX , fox , and mex R, were prevalent in 100%, 80%, and 100% of the isolates, respectively. PCR confirmed virulence genes such as tox A, exo Y, las B, and las I in 100%, 60%, 80%, and 80% of the isolates, respectively. Nucleotide sequence analysis of representative resistance gene ( mex R gene) and virulence genes ( tox A and las I genes) revealed a high correlation between P. aeruginosa recovered from pericarditis in broiler chickens in the present study with PAO1 (reference strain) and with other sequences published on the GenBank representing different localities worldwide. Conclusion: It could be concluded that P. aeruginosa recovered from pericarditis in broiler chickens in the current study is highly virulent bacteria, resisting most of the therapeutic agents which not only bear hazards for poultry industry but also represent a public health concern.

Effect of garlic oil nanoemulsion against multidrug resistant Pseudomonas aeruginosa isolated from broiler

“…In agreement with our study, the positive zeta potentials were significantly associated with nano-delivery system uptake across the mucosa (58). Furthermore, garlic oil nanoemulsions of mean size 36.3 nm, of average zeta potential was -26.23 mV (with different ratio of surfactant) was reported, and polydispersity index was 0.527 showed a weak antibacterial effect on some Gramnegative bacteria (59). Our study showed that garlic oil nanoemulsion's low MIC value revealed an antibacterial effect (partial bioactivity) against ten P. aeruginosa isolates.…”

Occurrence, antimicrobial susceptibility, and pathogenic factors of Pseudomonas aeruginosa in canine clinical samples

“…The extracellular pathogenic factors play a key role in P. aeruginosa infection and the most investigated are represented by exotoxin A, phospholipase C, elastase, alkaline protease, and type III secretion system [61]. Few data are available in veterinary literature about the occurrence of these factors in P. aeruginosa clinical isolates from companion [62] and farm animals [63][64][65]. In the present study, a high occurrence was found for all the pathogenic markers that we investigated, suggesting the elevated virulence potential of the isolates, as well as reported in human clinical isolates [66][67][68].…”

Phenotypic Characterization of Extensively Drug Resistant Pseudomonas aeruginosa (XDR) from Broiler Chickens in Sharkia Province, Egypt

“…(Tawakol et al, 2018) reported that P. aeruginosa isolates were highly sensitive to colistin sulphate and amikacin (90%) for each, and (Hassan et al, 2020) recorded that the most effective antibiotics were imipenem and colistin (100% for each), and amikacin (92%). Colistin is considered a significant option for treatment of MDR-resistant P. aeruginosa infections due to its bactericidal effect (Gill et al,…”