Virulence Attributes and Antibiotic Resistance Pattern of E. coli Isolated from Human and Animals

Pandey, Anshu; Joshi, Namita; Joshi, Rajesh K.; Prajapati, R. B.; Singh, Ankita

doi:10.3923/ajava.2016.67.72

Cited by 5 publications

(5 citation statements)

References 14 publications

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“…Studies conducted on E. coli isolates exhibited 75% (Gharieb et al 2019), 83% (Wani et al 2013) and 87% (Gupta et al 2017) resistance for ampicillin. Several studies also reported resistance toward ampicillin as 100% in E. coli isolates from calves (Donaldson et al 2006;Mailk et al 2013;Pandey et al 2015;Mohammed et al 2019). With respect to tetracycline resistance, the resistance level indicated in this study (54.3%) also corroborated earlier studies mostly ranging from 57% (Hang et al 2019) to 63% (Srivani et al 2017;Maciel et al 2019).…”

Section: Discussionsupporting

confidence: 88%

Pathotyping and antimicrobial susceptibility testing of Escherichia coli isolates from neonatal calves

et al. 2021

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Section: Discussionsupporting

confidence: 88%

Pathotyping and antimicrobial susceptibility testing of Escherichia coli isolates from neonatal calves

et al. 2021

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“…In India, there are reports of 92.9% resistance of E. coli isolates to ampicillin in children [19] and resistance of 80.43% in poultry in Eastern India [20] which also were particularly true with our finding; however, their finding of high resistance to cefotaxime (47.5%) disagree with our report in which we could detect only 9.25% resistance against it. Also, Pandey et al, [21] reported 100% resistance of E. coli isolates of pigs to cephalexin and ampicillin. We reported high susceptibility of the isolates to ciprofloxacin (91.6%) and gentamicin (88.02%) which agreed with reports of other scientists [21,20] where ciprofloxacin was the drug of choice as far as susceptibility is concerned and high susceptibility to ciprofloxacin that has been observed is consistent with the finding of other authors [22].…”

Section: Resultsmentioning

confidence: 99%

“…Also, Pandey et al, [21] reported 100% resistance of E. coli isolates of pigs to cephalexin and ampicillin. We reported high susceptibility of the isolates to ciprofloxacin (91.6%) and gentamicin (88.02%) which agreed with reports of other scientists [21,20] where ciprofloxacin was the drug of choice as far as susceptibility is concerned and high susceptibility to ciprofloxacin that has been observed is consistent with the finding of other authors [22]. There is a variation in resistance to streptomycin, ampicillin, cefotaxime, ceftriaxone and ceftazidime as compared to findings of Sasirekha et al, [23].…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial Drug Resistance of Escherichia coli Isolated from Piglets in North East India

Kylla¹,

Dutta

Roychoudhury

et al. 2020

CJAST

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Aim: The research is aimed to study the antimicrobial drug resistance (AMR) of Escherichia coli from fecal samples of pre-weaned piglets in North Eastern states (NE) of India. Materials and Methods: A total of 457 fresh fecal samples were collected from pre-weaned piglets of organized (n=225) and unorganized (n=232) farms of 4 North Eastern states of India, namely, Manipur, Meghalaya, Mizoram and Nagaland state. Samples were collected from diarrheic (n=339) and non-diarrheic (n=118) piglets in different seasons during the study period. The samples were processed for isolation of E. coli and detection of their putative virulence genes by polymerase chain reaction (PCR). Results: A total of 1286 E. coli were isolated. Forty-two isolates (3.26%) were found to be atypical enteropathogenic E. coli (aEPEC) and thirty isolates (2.33%) belongs to shigatoxigenic E. coli (STEC) in which 28 isolates were positive for stx2 gene and two isolates possessed hlyA gene. All the 1286 isolates showed wide variation (0.15-78.69%) in resistance pattern against the 15 antimicrobial agents; of which there is higher resistance against cefalexin (78.69%), amoxycillin (77.13%), ampicillin (72.31%) and enrofloxacin (60.73%). The isolates showed low resistance to imipenem (0.15%), ceftriaxone (8.32%), ciprofloxacin (8.39%) and streptomycin (8.94%). Higher prevalence of AMR to numerous antimicrobials in this study was observed in isolates of organized farm compared to unorganized farming system. Conclusion: The present study exhibited variation in AMR in different NE states of India as well as in different farming system which indicate that drug consumption and resistance are closely related.

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“…Escherichia coli dilaporkan sebagai penyebab penyakit pada sapi (Hogan et al, 1990), ayam broiler (Ruaa at al., 2014), unggas, babi dan manusia (Pandey et al, 2016). Pada manusia menyebabkan Hemolytic Uremic Syndrome (sindrome infeksi saluran kencing berdarah) (Pullanhi et al, 2019;Kaira dan Pai, 2018;Divakara et al., 2017).…”

Section: Pendahuluanunclassified

“…Hogan et al (1990) melaporkan adanya perbedaan hasil uji hemaglutinasi dengan menggunakan eritrosit sapi dengan marmut. Hasil yang berbeda dengan menggunakan eritrosit sapi, babi, manusia dan ayam (Pandey et al, 2016) Hemaglutinin merupakan faktor virulensi pada E. coli yang berperan sebagai faktor adhesin, kolonisasi dipermukaan sel epitel dan pelekatan bakteri pada permukaan sel epitel inang (Chanter et al, 1993). Adhesi bakteri dipermukaan sel epitel inang merupakan langkah awal dari proses infeksi (Mulvey, 2002), baik bakteri patogen maupun non patogen (Haiko dan Westerlund-Wikström, 2013).…”

Section: Uji Fagositosis Netrofilunclassified

Deteksi Faktor Virulensi Secara Fenotip pada Escherichia coli Isolat Susu Mentah Sapi Perah

Khusnan¹,

Purnomo²

2022

jveteriner

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Susu sapi merupakan media pertumbuhan bakteri patogen maupun non patogen. Escherichia coli mrupakan salah satu bakteri yang sering ditemukan pada susu sapi mentah. Escherichia coli patogen pada sapi dapat menyebabkan mastitis maupun radang usus serta dapat menyebabkan masalah kesehatan pada manusia seperti diare, kolitis hemoragik dan sindrom uraemik hemolitik. Hemaglutinin dan hemolisin merupakan faktor virulen yang penting pada E. coli patogen, karena berperan sebagai faktor adhesin, dan faktor pertahanan terhadap fagositosis. Tujuan penelitian ini mendeteksi hemaglutinin dan hemolisin E. coli isolat asal susu sapi mentah serta kemampuan adhesi pada sel epitel serta pertahanan terhadap fagosit netrofil. Hasil penelitian ini menunjukkan 100% isolat E. coli tidak memiliki hemaglutinin dan 17,2% merupakan E. coli hemolitik. Pada uji adhesi mampu melekat pada sel epitel bukalis 46,87 bakteri/sel. Pada uji fagositosis isolat-isolat hemolitik lebih sedikit difagosit oleh neutrofil dibandingkan isolat non hemolitik (2,64 dibanding 3,1 bakteri) tiap neutrofil (P<0,05). Ditemukannya E. coli patogen pada susu mentah menegaskan pentingnya pasteurisasi pada susu sebelum dikonsumsi.

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Virulence Attributes and Antibiotic Resistance Pattern of E. coli Isolated from Human and Animals

Cited by 5 publications

References 14 publications

Pathotyping and antimicrobial susceptibility testing of Escherichia coli isolates from neonatal calves

Pathotyping and antimicrobial susceptibility testing of Escherichia coli isolates from neonatal calves

Antimicrobial Drug Resistance of Escherichia coli Isolated from Piglets in North East India

Deteksi Faktor Virulensi Secara Fenotip pada Escherichia coli Isolat Susu Mentah Sapi Perah

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