Virulence determinants, biofilm production and antimicrobial susceptibility in Staphylococcus aureus causing device-associated infections in a Tunisian hospital

Mesrati, I.; Saïdani, Mabrouka; Jemili, M.; Ferjeni, Sana; Slim, Aminé; Boubaker, Ilhem Boutiba-Ben

doi:10.1016/j.ijantimicag.2018.05.004

Cited by 12 publications

(6 citation statements)

References 39 publications

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“…If Odc < Ods ≤ 2 × Odc, the sample is a weak biofilm producer (score = 1). If 2 × Odc < Ods ≤ 4 × Odc, the sample is a moderate biofilm producer (score = 2) and the sample is considered strong biofilm producer if 4 × Odc < Ods (score = 3) (Mesrati et al 2018 ).…”

Section: Methodsmentioning

confidence: 99%

Effect of spdC gene expression on virulence and antibiotic resistance in clinical Staphylococcus aureus isolates

et al. 2022

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Surface protein display C (SpdC) protein was described as a novel virulence factor of Staphylococcus aureus that affects biofilm formation and pathogenesis and favors resistance to antimicrobials targeting cell wall. We evaluated the possible correlation between spdC gene expression level and virulence as well as antibiotic resistance phenotypes in S. aureus clinical isolates. The antimicrobial susceptibility of S. aureus clinical isolates (n = 100) was determined by the disk diffusion method. Vancomycin susceptibility was determined by the broth microdilution method. The level of the extracellular proteases and delta-hemolysin was evaluated by measuring the proteolysis and hemolysis zone diameters in skim milk and blood agar plates, respectively. Biofilm formation was assayed using the 96-well microtiter plate method. Most of the isolates (81%) were multidrug-resistant and about half of the isolates (49%) were methicillin-resistant S. aureus. Hemolysin, protease, and biofilm production were detectable in 79%, 71%, and 96% of the isolates. No significant correlation was detectable between the level of spdC gene expression and the activity of tested virulence factors or the antimicrobial resistance phenotype. Therefore, the role of SpdC protein as a virulence regulator in S. aureus needs further evaluation together with the determination of the predominant regulators for each virulence factor.

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Section: Methodsmentioning

confidence: 99%

Effect of spdC gene expression on virulence and antibiotic resistance in clinical Staphylococcus aureus isolates

et al. 2022

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“…In terms of its antibacterial action, nisin functions similarly to glycopeptide antibiotics. Nisin binds to lipid II to form a complex, which prevents the normal synthesis of peptidoglycan and, as a result, prevents the formation of bacterial cell walls (49). Additionally, nisin has the ability to produce transmembrane pores that release cytoplasmic components, dissipate proton-motive force, and alter certain energy-dependent cellular functions, ultimately killing bacteria (50).…”

Section: Cytokine Assaymentioning

confidence: 99%

Enhanced Antibacterial and Antibiofilm Effects of Thiolated Chitosan-Encapsulated Nisin and Selenium Nanoparticles: A Potential Nanotechnology-Based Solution for Antibiotic- Resistant Bacterial Infections

Sefidi,

Bakhshi

2023

Preprint

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Nanotechnology holds significant promise in the battle against antibiotic-resistant bacteria. The aim of this study was to assess the bactericidal and antibiofilm effects of nisin and selenium encapsulated within thiolated chitosan (TC), a biodegradable and nontoxicderivative of chitosan, against common multidrug-resistant clinical enteric pathogens, including Vibrio cholerae O1 Eltor, Campylobacter jejuni, Salmonella enterica subsp. enterica, Shigella dysenteriae, Escherichia coli O157:H7, Listeria monocytogenes, andStaphylococcus aureus, using in vitro evaluations. TC-nisin-selenium nanoparticles (TCs-N-SeNPs) were synthesized using ionic cross-linking,and their formation was confirmed through various tests. The synthesized nanoparticles were subjected to assessment of their antibacterial properties and antibiofilm activity using micro broth dilution and the crystal violet method, respectively. Furthermore, their potential inflammatory and anti-inflammatory properties were examined by measuring the release of the cytokines TNF-α, IL-6, IL-10, and TGF-β in vitro. Nanoparticle synthesis confirmatory tests, along with the brick red color, validatedthe synthesis process and confirmedthe formation of TCs-N-SeNPs. The primary result indicates that TC-N-SeNPs exhibited a significant antibacterial and antibiofilm effect at concentrations ranging from 6.25 to 200 μg/mL. Due to disulfide linkages that keep nisin and selenium from being destroyed by lower pH, the effective release of nanosystems at alkaline pH is enhanced. The synergistic impact of uniform TCs-N-SeNPs compared to TCs-NNPs and TCs-SeNPs was evaluated successfully. In conclusion, TCs-N-SeNPs have promising potential as an effective therapeutic approach against antibiotic-resistant bacteria, with synergistic action, improved stability and enhanced bioavailability, antibacterial and antibiofilm properties.

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“…Bacteria can adhere to various metals (59) and biofilm formation may be different than on nonmetal material. However, irrespective of the method applied, several studies have demonstrated differences in biofilm production of the same species within a sample collection (60,61). Post et al demonstrated that, in a collection of isolates obtained from orthopedic implant-related infections, PJI isolates were more frequently strong biofilm formers than were isolates from fracture fixation-device infections (60).…”

Section: Differences In Biofilm Productionmentioning

confidence: 99%

Precision Medicine in the Diagnosis and Management of Orthopedic Biofilm Infections

Baldan

Sendi

2020

Front. Med.

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Virulence determinants, biofilm production and antimicrobial susceptibility in Staphylococcus aureus causing device-associated infections in a Tunisian hospital

Cited by 12 publications

References 39 publications

Effect of spdC gene expression on virulence and antibiotic resistance in clinical Staphylococcus aureus isolates

Effect of spdC gene expression on virulence and antibiotic resistance in clinical Staphylococcus aureus isolates

Enhanced Antibacterial and Antibiofilm Effects of Thiolated Chitosan-Encapsulated Nisin and Selenium Nanoparticles: A Potential Nanotechnology-Based Solution for Antibiotic- Resistant Bacterial Infections

Precision Medicine in the Diagnosis and Management of Orthopedic Biofilm Infections

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