Virulence determinants other than coronatine in Pseudomonas syringae pv. tomato PT23 are plasmid-encoded

Sesma, Ane; Aizpún, M.Teresa; Ortiz‐Barredo, Amaia; Arnold, Dawn L.; Vivian, Alan; Murillo, Jesús

doi:10.1006/pmpp.2000.0316

Cited by 14 publications

(7 citation statements)

References 47 publications

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“…pPT23A-like plasmids are of particular agricultural importance, as they commonly encode virulence and virulence-associated genes such as type III effectors (2,13,39,45,70,82). Plasmid pPMA4326A does not appear to encode any identifiable virulence genes, but it does possess several putative transposases.…”

Section: Resultsmentioning

confidence: 99%

Nucleotide Sequence and Evolution of the Five-Plasmid Complement of the Phytopathogen Pseudomonas syringae pv. maculicola ES4326

Stavrinides

Guttman

2004

J Bacteriol

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Plasmids are transmissible, extrachromosomal genetic elements that are often responsible for environmental or host-specific adaptations. In order to identify the forces driving the evolution of these important molecules, we determined the complete nucleotide sequence of the five-plasmid complement of the radish and Arabidopsis pathogen Pseudomonas syringae pv. maculicola ES4326 and conducted an intraspecific comparative genomic analysis. To date, this is the most complex fully sequenced plasmid complement of any gram-negative bacterium. The plasmid complement comprises two pPT23A-like replicons, pPMA4326A (46,697 bp) and pPMA4326B (40,110 bp); a pPS10-like replicon, pPMA4326C (8,244 bp); and two atypical, replicase-deficient replicons, pPMA4326D (4,833 bp) and pPMA4326E (4,217 bp). A complete type IV secretion system is found on pPMA4326A, while the type III secreted effector hopPmaA is present on pPMA4326B. The region around hopPmaA includes a shorter hopPmaA homolog, insertion sequence (IS) elements, and a three-element cassette composed of a resolvase, an integrase, and an exeA gene that is also present in several human pathogens. We have also identified a novel genetic element (E622) that is present on all but the smallest plasmid (pPMA4326E) that has features of an IS element but lacks an identifiable transposase. This element is associated with virulence-related genes found in a wide range of P. syringae strains. Comparative genomic analyses of these and other P. syringae plasmids suggest a role for recombination and integrative elements in driving plasmid evolution.

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Section: Resultsmentioning

confidence: 99%

Nucleotide Sequence and Evolution of the Five-Plasmid Complement of the Phytopathogen Pseudomonas syringae pv. maculicola ES4326

Stavrinides

Guttman

2004

J Bacteriol

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“…With one exception, the rulAB determinant was encoded on a plasmid that also cross‐hybridized with the oriV ‐pPT23A replication sequences. Such plasmids are members of the pPT23A family of plasmids in P. syringae , a plasmid family that encodes several determinants important to the ecology of individual P. syringae pathovars, including avirulence determinants that affect host range (Vivian et al ., 1997), production of extracellular virulence factors that enhance pathogenicity (Bender et al ., 1989; Jackson, 1997; Sesma et al ., 1997) and genes encoding resistance to copper and streptomycin bactericides that increase cell survival on plant hosts sprayed with these compounds (Sundin and Bender, 1996).…”

Section: Discussionmentioning

confidence: 99%

Functional analysis of the Pseudomonas syringae rulAB determinant in tolerance to ultraviolet B (290–320 nm) radiation and distribution of rulAB among P. syringae pathovars

Sundin

Murillo

1999

Environmental Microbiology

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The effect of the plasmid-encoded rulAB (resistance to ultraviolet radiation) determinant on responses of Pseudomonas syringae to ultraviolet-B (UV-B) radiation and the distribution of rulAB among pathovars of P. syringae were determined. The cloned rulAB determinant and the native rulAB+ plasmid pPSR1 both conferred approximately a 10-fold increase in survival on P. syringae pv. syringae FF5 following increasing doses of UV-B radiation. rulAB+ P. syringae strains also maintained significantly larger epiphytic populations on leaf surfaces irradiated with UV-B. rulAB-insertional mutants, constructed in two native rulAB+ strains, were from 10- to 100-fold more sensitive to UV-B radiation. The UV tolerance phenotype and the rulAB genes were widely distributed among P. syringae pathovars isolated from varied plant hosts throughout the world and within a broad range of genotypic backgrounds of P. syringae pv. syringae. With one exception, the rulAB determinant was harboured on pPT23A-like plasmids; these replicons are indigenous residents of the species P. syringae and also tend to encode determinants of importance in host-pathogen interactions.

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“…In contrast to AvrB and AvrA1, AvrD1 is not known to exhibit virulence activity, at least in tomato (Sesma et al, 2001). Therefore, we first tested if Psg avrD1 showed increased virulence in rpg4 (cv Merit) soybean plants.…”

Section: Gmndr1 Proteins Bind and Affect Virulence Activities Of Avrbmentioning

confidence: 99%

Soybean NDR1‐like proteins bind pathogen effectors and regulate resistance signaling

et al. 2013

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SummaryNonrace specific disease resistance 1 (NDR1) is a conserved downstream regulator of resistance (R) protein-derived signaling. We identified two NDR1-like sequences (GmNDR1a, b) from soybean, and investigated their roles in R-mediated resistance and pathogen effector detection.Silencing GmNDR1a and b in soybean shows that these genes are required for resistance derived from the Rpg1-b, Rpg3, and Rpg4 loci, against Pseudomonas syringae (Psg) expressing avrB, avrB2 and avrD1, respectively. Immunoprecipitation assays show that the GmNDR1 proteins interact with the AvrB2 and AvrD1 Psg effectors. This correlates with the enhanced virulence of Psg avrB2 and Psg avrD1 in GmNDR1-silenced rpg3 rpg4 plants, even though these strains are not normally more virulent on plants lacking cognate R loci.The GmNDR1 proteins interact with GmRIN4 proteins, but not with AvrB, or its cognate R protein Rpg1-b. However, the GmNDR1 proteins promote AvrB-independent activation of Rpg1-b when coexpressed with a phosphomimic derivative of GmRIN4b.The role of GmNDR1 proteins in Rpg1-b activation, their direct interactions with AvrB2/ AvrD1, and a putative role in the virulence activities of Avr effectors, provides the first experimental evidence in support of the proposed role for NDR1 in transducing extracellular pathogen-derived signals.

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Virulence determinants other than coronatine in Pseudomonas syringae pv. tomato PT23 are plasmid-encoded

Cited by 14 publications

References 47 publications

Nucleotide Sequence and Evolution of the Five-Plasmid Complement of the Phytopathogen Pseudomonas syringae pv. maculicola ES4326

Nucleotide Sequence and Evolution of the Five-Plasmid Complement of the Phytopathogen Pseudomonas syringae pv. maculicola ES4326

Functional analysis of the Pseudomonas syringae rulAB determinant in tolerance to ultraviolet B (290–320 nm) radiation and distribution of rulAB among P. syringae pathovars

Soybean NDR1‐like proteins bind pathogen effectors and regulate resistance signaling

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