Virulence determination of Beauveria bassiana isolates on a predatory hemipteran, Andrallus spinidens Fabricius (Hemiptera: Pentatomidae)

Abstract

Virulence of the two isolates of Beauveria bassiana, BB 2 and AM-118, were evaluated on adults of a predatory hemipteran, Andrallus spinidens Fabricius by conidial bioassay and enzymatic activities. Results of the bioassay revealed LC 50 of 37×10 4 and 15×10 3 spore/ml for isolates BB 2 and AM-118, respectively. Activities of chitinase, lipase and ALP showed the higher activity in the media inoculated by AM-118 while no statistical differences were observed in activity of ACP. Although no statistical differences were found in general protease and Pr 1 activities but activity of Pr 2 in AM-118 was significantly higher than that of BB 2 . Activity of general esterases demonstrated no statistical differences when α-and β-naphtyl acetate were used as substrates but activity of glutathione S-transferase in AM-118 was higher than that of BB 2 by using CDNB and DCNB as specific reagents. Results of the current study indicated higher virulence of isolate AM-118 against adults of A. spinidens by lower LC 50 value and higher activities of the enzymes involved in pathogenicity. Recruiting of these isolates against C. suppressalis must be considered by their adaptability of A. spinidens. Moreover, AM-118 has been isolated from rice fields of northern Iran, so it may somehow indicate a type of host-microorganism interaction.

The abundance of canopy arthropods in South Sumatra (Indonesia) freshwater swamp main and ratooned rice applied with bioinsecticides and synthetic insecticide

“…While the entomopathogenic fungi had specific host insects, specifically the lepidopteran (Safitri et al 2018;Ayudya et al 2019), whereas in this study the dominant arthropods were spiders and insects of coleopteran. The entomopathogenic fungi were harmless to spiders (Yuliani 2016), Coleoptera (Ngin et al 2017), and Hemiptera (Firouzbakht et al 2015;Gholamzadeh-Chitgar et al 2017).…”

Purification and characterization of the cuticle-degrading proteases produced by an isolate of Beauveria bassiana using the cuticle of the predatory bug, Andrallus spinidens Fabricius (Hemiptera: Pentatomidae)

“…Understanding the potential adaptability of the two biocontrol agents is crucial to preventing conflicts in an agro-ecosystem. Therefore, we are interested in the virulence of two isolates of B. bassiana -BB2 and AM-118 -on A. spinidens (Safavi 2010;Chitgar et al 2014;Firouzbakht et al 2015). In this context, we have conducted the current study by extracting and purifying Pr1 and Pr2 proteases produced by isolate AM-118.…”

The impact of bioinsecticide overdoses of Beauveria bassiana on species diversity and abundance of not targeted arthropods in South Sumatra (Indonesia) freshwater swamp paddy

“…B. bassiana can kill paddy insects such as brown plant hoppers (BPH) (Li et al 2014;Sumikarsih et al 2019), Spodoptera litura (Ayudya et al 2019). The research results in the laboratory proved that B. bassiana did not harm the natural enemies of BPH (non-target arthropods) (Firouzbakht et al 2015;Gholamzadeh-Chitgar et al 2017). However, caution is needed in applying the bioinsecticide because the non-target arthropod species is very abundant, rich, and diverse in the paddy ecosystem of freshwater swamps in South Sumatra, such as spiders (Herlinda et al 2018), predatory insects, parasitoids, and neutral insects (Herlinda et al 2019b;Karenina et al 2019) if an overdose can affect the abundance and diversity of non-target arthropods.…”