Virulence factors (aerobactin and mucoid phenotype) in and blood culture isolates

Vernet, V.; Philippon, A.; Madoulet, Claudie; Vistelle, Richard; Jaussaud, R.; Chippaux, C

doi:10.1016/0378-1097(95)00141-q

Cited by 15 publications

(20 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[27][28][29][30] Compared with other Enterobacteriaceae, K. pneumonia is the most concerning pathogen for its severe morbidity and mortality. 31 Tsukadaira et al 32 reported four cases of K. pneumonia infections, which were typical lobar pneumonia (Friedlander pneumonia), acute bronchopneumonia with subclinical aspiration, and chronic K. pneumonia with typical cavitary lung abscesses. E. coli, K. pneumoniae, and Enterococcus faecalis, which are often detected in the wastewater treatment process, can be used as indicators for pathogenic microorganisms in culture-based detection.…”

Section: Pathogen/primer Dna Sequence (5-3) Referencementioning

confidence: 98%

Quantification and Analysis of Airborne Bacterial Characteristics in a Nursing Care Institution

Feng

Sung

et al. 2011

Journal of the Air & Waste Management Association

View full text Add to dashboard Cite

Indoor air quality has become a critical issue because people spend most of their time in the indoor environment. The factors that influence indoor air quality are very important to environmental sanitation and air quality improvement. This study focuses on monitoring air quality, colony counts, and bacteria species of the indoor air of a nursing care institution. The regular colony counts in two different wards range from 55 to 600 cfu m(-3) Regression analysis results indicate that the bacterial colony counts have close correlation with relative humidity or carbon dioxide (CO2) but not with carbon monoxide (CO) or ozone (O3). Real-time PCR was used to quantify the bacterial pathogens of nosocomial infection, including Acinetobacter baumannii, Citrobacter freundii, Escherichia coli, Klebsiella pneumoniae, and methicillin-sensitive Staphylococcus aureus. The most abundant bacteria species in the air of the nursing care institution is E. coli.

show abstract

Section: Pathogen/primer Dna Sequence (5-3) Referencementioning

confidence: 98%

Quantification and Analysis of Airborne Bacterial Characteristics in a Nursing Care Institution

Feng

Sung

et al. 2011

Journal of the Air & Waste Management Association

View full text Add to dashboard Cite

show abstract

“…Nevertheless, the problem of sampling the cervix of the uterus is that a speculum exam is needed which is not easy to perform in women in labor. The most specific molecular target for invasive E. coli , other than the serotype K1 itself, was the gene for the iron uptake aerobactin system [11,26,27] . This target was used to detect what we define as invasive E. coli strains, based on our studies on a collection of pathogen isolates.…”

Section: Discussionmentioning

confidence: 99%

Development of a Rapid PCR Assay for Screening of Maternal Colonization by Group B <i>Streptococcus</i> and Neonatal Invasive <i>Escherichia coli</i> during Labor

Tejada¹,

Stan²,

Boulvain³

et al. 2010

Gynecol Obstet Invest

View full text Add to dashboard Cite

Objective: Group B Streptococcus (GBS) and Escherichiacoli(E. coli) are the leading causes of early-onset neonatal disease (EOD). Intrapartum antibiotic prophylaxis of GBS-colonized women decreases vertical transmission and EOD due to GBS. Nevertheless, no intervention has been developed to reduce the risk of EOD related to E. coli. Timely and accurate identification of colonized mothers is necessary to implement preventive strategies against neonatal sepsis. To screen for colonization during labor, we developed a real-time PCR assay for the simultaneous detection of GBS and neonatal invasive strains of E. coli. Study Design: Specific DNA targets for GBS are publicly available. For neonatal invasive E. coli, we analyzed candidate DNA targets by DNA hybridization on microarrays of invasive strains isolated from neonatal E. coli sepsis or meningitis (K1 and not K1 ‘invasive’ serotypes). Specificity of DNA probes was tested against a panel of bacteria and by simulating clinical conditions (spiking vaginal samples from pregnant women). Then, the characteristics of the selected probes were evaluated in a pilot study including 200 women in labor. Results: Prevalence of rectovaginal GBS and of vaginal and cervical E. coliserotype K1 colonization were 16.0, 3.5 and 3.5% by culture and 27, 10 and 8.5% by PCR, respectively. The prevalence of other invasive E. coli in the vagina and in the cervix, detected by PCR, was around 10%. Compared to the culture, considered as the gold standard, the sensitivities of the PCRs for the GBS and E. coli K1 were 97 and 71%, respectively. Specificities were 86 and 92%, respectively. Specificity is difficult to interpret, as a false-positive PCR result may in fact be a false-negative result of the culture. The turnaround time needed for PCR analysis was 2.5 h, compared to a minimum of 48 h for the culture. Conclusion: Our rapid PCR is reliable in detecting GBS in women in labor. Optimization of the PCR for invasive E. coli is needed before its implementation in clinical practice. More efforts to fight against main causes of neonatal sepsis need to be undertaken to prevent this terrible medical complication.

show abstract

“…pneumoniae was included in the hazard identification category "B" by FAO and WHO on microorganisms, in powdered infant formula, which are considered to be an emerging public health issue (FAO-WHO, 2014). In comparison to other Enterobacteriaceae in the category "B", K. pneumoniae was the most important pathogen due to its severe morbidity and mortality (Vernet et al, 1995). Klebsiella species can cause severe infections that include meningitis, bronchitis, bacteremia, pneumonia, and urinary tract infections in humans and animals (Siri et al, 2011).…”

Section: Introductionmentioning

confidence: 98%

Effect of radiation processing in elimination of Klebsiella pneumoniae from food

Gautam

Nagar

Shashidhar

2015

Radiation Physics and Chemistry

View full text Add to dashboard Cite

Virulence factors (aerobactin and mucoid phenotype) in and blood culture isolates

Cited by 15 publications

References 28 publications

Quantification and Analysis of Airborne Bacterial Characteristics in a Nursing Care Institution

Quantification and Analysis of Airborne Bacterial Characteristics in a Nursing Care Institution

Development of a Rapid PCR Assay for Screening of Maternal Colonization by Group B <i>Streptococcus</i> and Neonatal Invasive <i>Escherichia coli</i> during Labor

Effect of radiation processing in elimination of Klebsiella pneumoniae from food

Contact Info

Product

Resources

About