Virulence factors and antibiotic susceptibility patterns of multidrug resistance Klebsiella pneumoniae isolated from different clinical infections

Abstract

Many strains of Klebsiella pneumoniae can produce several virulence factors, such as siderophores, capsules, fimbrial adhesins and antibiotic resistance enzymes, which are important in the adherence, colonization, invasion and development of the infection. Therefore the aim of this study was to investigate ten virulence gens in 32 K. pneumoniae isolated from different clinical infections: urine (13), burns (12), sputum (4) and blood (3). Phenotypic methods were used to detect capsule, hypermucoviscosity, ability to form biofilm, produce siderophores, primary and confirmatory test of extended spectrum beta lactamase (ESBL). Polymerase chain reaction (PCR) technique was used to detect ten virulence genes encoding attachments (fimH, ycfM), siderophores (kfu: iron uptake system, entB: enterobactin, irp-2: yersiniabactin), capsule synthesis or invasions (rmpA, uge, wabG) and beta lactamase enzymes (SHV, TEM). Antimicrobial susceptibility was tested by disk diffusion method. According to phenotypic methods, the results demonstrated that, the capsule and siderophores production and biofilm formation were observed in 100% of isolates, while hypermucoviscosity phenomenon was observed in 62.5%. The most common virulence genes were fimH-1, ycfM and entB (100%), uge and TEM (93.75%), wabG and SHV (87.5%). Kfu and rpmA genes were found at medium rates of 65.62 and 62.5%, respectively and at lower prevalence was gene Irp-2 (37.5%). A rate of 84.375% of isolates showed a multidrug resistance (MDR) pattern, 12.5% extensive drug resistance (XDR), 3.12% pandrug resistance (PDR) and the rate of extended-spectrum β-lactamases (ESBL) producing K. pneumoniae was 62.5%.The study concluded that fimH-1, ycfM and entB genes were commonly found in all isolates, they seem to be at the basis of classic pathogenicity of K. pneumoniae. The most common virulence genes were observed in isolates from burns and blood samples. K. pneumoniae became highly resistant to antibiotics especially to 3 rd generation cephalosporins and there was positive relationship between presence of virulence genes and ability of the bacterium to antibiotics resistance.

Association between Virulence Factors and Extended Spectrum Beta-Lactamase ProducingKlebsiella pneumoniaeCompared to Nonproducing Isolates

“…In the study of Shin and Ko, 2014, 33.6% of the isolates were ESBLs producer [32]. A higher percent was found in Arabian region where Aljanaby and Alhasani, 2016, reported that rate of ESBL producing K. pneumoniae was 62.5% in AL-Najaf Governorate, Iraq [33]. In this study, 50% of isolates were estimated as ESBLs producers.…”

“…In this study, PCR detection of these genes revealed that 100, 96, and 84% of ESBL producing K. pneumoniae harbored C TX-M- 15 , SHV, and TEM, respectively (Table 2). Shin and Ko, 2014, showed similar results regarding CTX-M where all ESBL producers were found to harbor bla CTX-M gene [33]. Additionally, Aljanaby and Alhasani, 2016, reported that TEM and SHV were 93.75% (30/32) and 87.5% (28/32), respectively [33].…”

“…Shin and Ko, 2014, showed similar results regarding CTX-M where all ESBL producers were found to harbor bla CTX-M gene [33]. Additionally, Aljanaby and Alhasani, 2016, reported that TEM and SHV were 93.75% (30/32) and 87.5% (28/32), respectively [33]. …”

Detection of blaTEM Gene of Klebsiella pneumoniae Isolated from Swab of Food-Producing Animals in East Java

“…Research conducted by Ahmed & Shimamoto (2011) shows that bla TEM genes as antimicrobial resistance found as many as 23 isolates (67. 6%) of 34 isolates Gram-negative in case of mastitis in Egypt, and research conducted by Aljanaby & Alhasani (2016) also showed 30 isolates (93.75%) found bla TEM genes from 32 bacterial isolates K. pneumoniae isolated from patients with different clinias infections in Iraq. The majority of ESBL enzymes derived from the TEM type decoded by gene bla TEM .…”

Antibacterial Effect of Seidlitzia rosmarinus Extract and Silver Nanoparticles on Staphylococcus aureus and Klebsiella pneumoniae Isolated from Urinary Tract Infections

“…For this purpose, susceptibility of S. aureus isolates was detected by Cefoxitin disk (30 µg) on Mueller Hinton Agar (Merck, Germany) and strains having ≤ 21 mm zone of inhibition were considered as MDR strains (18). MDR strains of K. pneumoniae were determined by combination disk method (Cefotaxime (30 µg) alone and with Clavulanic acid (10 µg)) on Mueller Hinton Agar (Merck, Germany).…”