Virulence factors and antimicrobial resistance of escherichia coli isolated from urinary tract of swine in southern of Brazil

Costa, Mateus Matiuzzi da; Drescher, Guilherme; Maboni, Franciele; Weber, Shana de Souto; Botton, Sônia de Ávila; Vainstein, Marilene Henning; Schrank, Irene Silveira; Vargas, Águeda Castagna de

doi:10.1590/s1517-83822008000400027

Cited by 8 publications

(7 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast with WHO recommendations for UTI treatment with SXT, many studies have proposed SXT as an inappropriate antibiotic for the treatment of UTI (12)(13)(14)(15) In addition, this report described a high prevalence (44z) of multidrug-resistant UPEC isolates from Iran, which is similar to our observations (14). In one study, the frequency of UPEC strains resistant to neomycin (37.8z) was less than that of the other studied antibiotics (17). According to the present study, due to highlevel resistance of isolates against AMC, SXT, and CTX, we conclude that these antibiotics are relatively ineffective for UTI treatment in Southern Iran.…”

contrasting

confidence: 47%

Virulence Characteristics and Antibiotic Resistance Patterns among Various Phylogenetic Groups of Uropathogenic <i>Escherichia coli</i> Isolates

et al. 2015

View full text Add to dashboard Cite

SUMMARY:The aim of this study was to determine the resistance patterns of uropathogenic Escherichia coli (UPEC) isolates and to investigate the frequency of several virulence genes, including fimH, papA, hlyD, cnf-1, sitA, and tsh, among various phylogenetic groups of UPEC isolates. A total of 85 E. coli isolates were recovered from urine samples from outpatients with a clinical diagnosis of uncomplicated urinary tract infections. A molecular approach to examine the antimicrobial resistance patterns was employed using PCR and the disc diffusion method. The detected frequencies of the virulence factor genes determined using PCR were: fimH (34.1z), papA (9.4z), hlyD (21.2z), cnf-1 (3.5z), sitA (15.3z), and tsh (27.1z). These results revealed that the isolates were resistant to trimethoprimsulfamethoxazole (SXT) (74.1z), cefotaxime (CTX) (68.2z), and amoxicillin-clavulanic acid (AMC) (94.1z), and they were relatively less resistant to N (56.5z). According to these results, further investigation is needed to determine exactly whether or not SXT, CTX, and AMC are appropriate antibiotics for the treatment of UPEC infections in southern Iran. Although these results demonstrate that fimH is the most frequent virulence gene among UPEC isolates, the high prevalence of isolates that do not encode fimH (75.9z) and the relatively low frequency of isolates that carry other virulence genes require further investigation to clarify the role of the other potential virulence factors in the pathogenesis of these isolates.

show abstract

contrasting

confidence: 47%

Virulence Characteristics and Antibiotic Resistance Patterns among Various Phylogenetic Groups of Uropathogenic <i>Escherichia coli</i> Isolates

et al. 2015

View full text Add to dashboard Cite

show abstract

“…The majority of urine isolates (62.8%) were negative for virulence factors when tested by mPCR, which is consistent with previous ϐindings (Costa et al 2008). The remaining 37.1% (26/70) were positive for one or more virulence factors, 20% of these (14/70) were classiϐied as UPEC, 12.8% (9/70) as both UPEC and ETEC and 5.8% (4/70) as ETEC (Fig.1A).…”

Section: Resultssupporting

confidence: 88%

“…The E. coli samples were genotyped using two mPCR assays to detect ϐimbria and toxins by amplifying the following gene encoding for regions: STa (158 bp), STb (113 bp), LT (272 bp), STx (758 bp), F4 (499 bp), F5 (230 bp), F6 (520 bp), F18 (313 bp) and F41 (613 bp), for the intestinal, fecal and tissue samples; and cnf (543 bp), hly (1117 bp), bfp (326 bp), eae (368 bp), pap (328 bp), iha (827 bp), sfa (410 bp), cnf (543 bp) and usp (440 bp) for the urinary isolates, as previously described (Brito et al 2004, Costa et al 2008, Blanco et al 1996, Blanco et al 1997 Aliquots of 10μl ampliϐication products were subjected to electrophoresis for 45 minutes at 100V in 1.5% agarose gel stained with ethidium bromide. DNA fragments were compared with a 50 bp DNA ladder.…”

Section: Methodsmentioning

confidence: 99%

“…In Brazil, there are a limited number of studies involving the molecular characterization of E. coli strains of swine origin (Brito et al 1999, Costa et al 2006, Costa et al 2008, Costa et al 2010 as well as its classiϐication into phylogenetic groups. The aims of the current study were the characterization of swine E. coli isolates by multiplex PCR (mPCR) and to classify these isolates into phylogenetic groups.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Phylogenetic and pathotype analysis of Escherichia coli swine isolates from Southern Brazil

et al. 2012

View full text Add to dashboard Cite

The current study evaluated the presence of virulence factors by a multiplex PCR technique and then phylogenetically classified the studied strains into groups A, B1, B2 and D, according to Clermont et al. (2000), in 152 intestinal and extraintestinal swine isolates of Escherichia coli. Seventy seven isolates tested were positive for virulence factors. Phylogenetic characterization placed 21 samples into group A, 65 into B1, 19 into B2 and 47 into D. Fourteen urine samples were classified as uropathogenic E. coli (UPEC), nine were both UPEC and enterotoxigenic E. coli (ETEC) and four were ETEC only. The most common phylogenetic classifications were B1 and D groups. Of the analyzed fecal samples, 25 were classified as ETEC. Phylogenetically, the group of higher occurrence was B1, followed by B2, A and D. For the small intestine samples, 20 were classified as ETEC. Phylogenetic analysis found groups B1 and A to be the most commons in these samples. Six isolated tissue samples were classified as ETEC and most of them were designated as group D by phylogenetic classification. The phylogenetic analysis could be employed in veterinary laboratories in the E. coli isolates screening, including the possibility of vaccine strain selection and epidemiological searches.

show abstract

“…Women are more likely to experience UTI than men. UTIs affect a large proportion of the world population and are responsible for significant morbidity and high medical costs (1, 2, 3, 45). …”

Section: Introductionmentioning

confidence: 99%

Multiple drug resistance patterns in various phylogenetic groups of uropathogenic E.coli isolated from Faisalabad region of Pakistan

Bashir¹,

Sarwar²,

Ali³

et al. 2011

Braz. J. Microbiol.

View full text Add to dashboard Cite

The objective of this work was the phylogenetic characterization of local clinical isolates of uropathogenic E. coli with respect to drug resistance. A total of 59 uropathogenic E. coli responsible for community acquired urinary tract infections were included in this study. A triplex PCR was employed to segregate each isolate into four different phylogenetic groups (A, B1, B2 and D). Drug resistance was evaluated by disc diffusion method. The drugs used were ampicillin, aztreonam, cefixime, cefoperazone, ceftriaxone, cephradine among β-lactam group; amikacin, gentamicin, and streptomycin among aminoglycosides; nalidixic acid and ciprofloxacin from quinolones; trimethoprim-sulfomethoxazole, and tetracycline. Among 59 uropathogenic E. coli isolates majority belonged to phylogenetic group B2 (50%) where as 19% each belonged to groups A and B1, and 12% to group D. All the isolates were multiple drug resistant (MDR). Most effective drugs against Group A, B1, and B2 were gentamicin, amikacin and cefixime; ceftriaxone and quinolones; and ceftriaxone and amikacin, respectively. Group D isolates were found to be highly resistant to all drugs. Our results have shown emergence of MDR isolates among uropathogenic E. coli with dominance of phylogenetic group B2. However, it was found that group D isolates were though less frequent, more drug resistant as compared with group B2. Groups A and B1 were relatively uncommon. Amikacin, ceftriaxone and gentamicin were the most effective drugs in general.

show abstract

Virulence factors and antimicrobial resistance of escherichia coli isolated from urinary tract of swine in southern of Brazil

Cited by 8 publications

References 22 publications

Virulence Characteristics and Antibiotic Resistance Patterns among Various Phylogenetic Groups of Uropathogenic <i>Escherichia coli</i> Isolates

Virulence Characteristics and Antibiotic Resistance Patterns among Various Phylogenetic Groups of Uropathogenic <i>Escherichia coli</i> Isolates

Phylogenetic and pathotype analysis of Escherichia coli swine isolates from Southern Brazil

Multiple drug resistance patterns in various phylogenetic groups of uropathogenic E.coli isolated from Faisalabad region of Pakistan

Contact Info

Product

Resources

About