Virulence Factors and O-Serogroups Profiles of Uropathogenic Escherichia Coli Isolated from Iranian Pediatric Patients

Abstract

Background:Uropathogenic Escherichia coli O- Serogroups with their virulence factors are the most prevalent causes of UTIs.Objectives:The present investigation was performed to study the virulence factors and O-Serogroups profiles of UPEC isolated from Iranian pediatric patients.Patients and Methods:This cross sectional investigation was performed on 100 urine samples collected from hospitalized pediatrics of Baqiyatallah Hospital, Tehran, Iran. Midstream urine was collected to decrease potential bacterial, cellular and artifactual contamination. All samples were cultured and those with positive results were subjected to polymerase chain reactions to detect pap, cnf1, afa, sfa and hlyA genes and various O- Serogroups.Results:We found that 37.5% of boys and 75% of girls had positive results for Escherichia coli. We also found that O1 (19.33%), O2 (13.33%), O6 (13.33%), O4 (11.66%), and O18 (11.66 %) were the most commonly detected Serogroups. Totally, the serogroup of 5% of all strains were not detected. In addition, all of these O- Serogroups were pap+, cnf1+, hlyA+, and afa+. Totally, pap (70 %), cnf1 (56.66 %), and hlyA (43.33 %) were the most commonly detected virulence genes in the both studied groups of children. The sfa (30 %) and afa (26.66 %) genes had the lowest incidence rates.Conclusions:Special health care should be performed on UTIs management in Iranian pediatric patients. Extended researches should be performed to evaluate relation between other O-Serogroups and virulent genes.

Distribution of Virulence Factors and Antimicrobial Resistance Properties of Uropathogenic Escherichia coli Isolated from Diabetic and Healthy Males Suffered from Urinary Tract Infections

“…Momtaz et al (2013) 8 represented that set1, fim, cnf1, papGIII and hlyA were the most commonly detected virulence factors in the UPEC strains of patients suffered from UTIs which was entirely similar to our findings. High prevalence of fim, hly, sfa, afa and also various types of pap genes were also reported by Dormanesh et al (2014) 15 24 . These genes are mainly associated with adhesion, colonization and invasion of bacterial strains into the urinary epithelial cells.…”

“…All of the positive colonies were confirmed using the polymerase chain reaction (PCR) technique (15). PCR was performed with a total volume of 50 µL including 2 mM MgCl2, 1 µM of forward primer (5'-AGAGTTTGATC MTGGCTCAG-3'), 1 µM of reverse primer (5'-CCGTCAATTCATTTGAGTTT-3'), 5 µL PCR buffer 10X, 200 µM dNTP (Fermentas), 1 U Taq DNA polymerase (Fermentas) and 2.5 µL DNA template.…”

Antibacterial effects of the methanolic extract of Glycine Max (Soybean)

“…3,4 Resistant bacteria caused more severe clinical diseases for longer period of time which causes abundant economic losses. [1][2][3][4] Documented data revealed that pathogenic bacteria and especially Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), Bacillus cereus (B. cereus), Pseudomonas aeruginosa (P. aeruginosa), Listeria monocytogenes (L. monocytogenes), Klebsiella pneumoniae (K. pneumoniae) and Salmonella typhi (S. typhi) exhibited the high levels of resistance against aminoglycosides, tetracyclines, lincosamides, macrolides, beta-lactams, quinolones, fluoroquinolone and cephems groups of antibiotics. [1][2][3][4][5][6][7][8] Therefore, therapeutic and pharmacological factories tried to use from novel sources for antimicrobial agents to produce strong antibiotic drugs.…”

“…Occurrence of severe levels of the antibiotic resistance is the main issue facing medical practitioners. 1,2 Antimicrobial resistance threatens the effective prevention and treatment of an ever-increasing range of infections caused by bacteria. It is an increasingly serious threat with global public health impact that requires action across all government sectors and society.…”

Prevalence of antibiotic resistance and blaIMP-1 gene among Pseudomonas aeruginosa strains isolated from burn and urinary tract infections in Isfahan, central Iran

“…[2][3][4] UTIs is an important cause of mortality and morbidity alaround the world. 5,6 Pseudomonas aeruginosa (P. aeruginosa) is a non-fermentative, aerobic, Gramnegative rod shape bacterium and is responsible for severe human clinical infections such as BIs, UTIs, pneumonia, wound, reproductive tract, respiratory tract and superficial and gastrointestinal infections, cystic fibrosis, ecthyma gangrenosum and black necrotic lesions. 7 It is also related to sever cases of hospital-acquired and healthcare associated infections globally.…”