Virulence factors, antibiotic resistance genes and genetic relatedness of commensal Escherichia coli isolates from dogs and their owners

Derakhshandeh, Abdollah; Eraghi, Vida; Boroojeni, Azar Motamedi; Niaki, Malihe Akbarzadeh; Zare, Sahar; Naziri, Zahra

doi:10.1016/j.micpath.2018.01.041

Cited by 25 publications

(21 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One pet owner and his dog completely showed the same virulence gene profile. Derakhshandeh et al (2018) in the Iran, Harada et al (2012) in the Japan and Stenske et al (2009) in the United States, reported that canine feces are a significant source of pathogenic E. coli strains for urinary tract infections for their owners (30)(31)(32). Based to our data pet dogs may be considered as a mild source of pathogenic and a major reservoir of commensal E. coli strains.…”

Section: Discussionsupporting

confidence: 58%

Detection of zoonotic diarrheagenic pathotypes of Escherichia coli in healthy household dogs

Askari¹,

Ghanbarpour²,

Akhtardanesh³

et al. 2020

IJM

View full text Add to dashboard Cite

Background and Objectives: Intestinal pathotypes of Escherichia coli belong to the companion animals may poses po- tential risk to public health following zoonotic transmission. Therefore, this study was proposed to determine the virulence genes associated to diarrheagenic E. coli strains isolated from healthy pet dogs and their owners in the southeast of Iran, Kerman province. Materials and Methods: Totally 168 E. coli isolates were collected from 49 healthy household dogs and their owners. Sev- enty isolates were obtained from non-pet owners as control group. Presence or absence of the virulence genes including eae, stx1, stx2, st1, lt1, ipaH, cnf1 and cnf2 were screened by conventional polymerase chain reaction (PCR) and dissemination pattern of the genes were studied among the various hosts. Results: PCR examinations showed that the most frequent virulence gene was ipaH (6.1%) in dogs followed by eae in dog owners (6.1%) and in controls (8.6%). The most frequent pathotypes in dogs, their owners and controls were EIEC (6.1%), EHEC (4.08%) and EPEC (8.5%), respectively. In one of studied houses, both of dog and its owner harbored E. coli strains with same virulence profile (stx1/eae) and pathotype (EHEC). Conclusion: These results collectively indicate that healthy household dogs probably are the mild reservoir of potential virulent E. coli strains with possible active transmission to their contact owner. However, even non-pet owners seemed to be a notable source of intestinal pathotypes, especially EPEC, for their environment. Transmission of E. coli pathotypes may occurs by direct contact with the reservoirs or ingestion of contaminated food. These pathotypes are potentially virulent and creates public health hazards. Further studies are needed for better understanding of dissemination mechanisms of E. coli pathotypes among humans and their pets.

show abstract

Section: Discussionsupporting

confidence: 58%

Detection of zoonotic diarrheagenic pathotypes of Escherichia coli in healthy household dogs

Askari¹,

Ghanbarpour²,

Akhtardanesh³

et al. 2020

IJM

View full text Add to dashboard Cite

show abstract

“…The gene catB3 was located together with the resistance genes aacA4 and dfrA1, and the gene cmlA6 was located together with the genes aadB and aadA1 in class 1 integrons of different sizes (194). In a study of 62 E. coli isolates from dogs in Iran, three isolates harbored the cmlA gene, whereas six isolates were positive for the floR gene (195). Among 36 chloramphenicol-and florfenicol-resistant E. coli isolates from dogs suffering from urinary tract infections in Taiwan, all isolates harbored the cmlA gene and 18 carried the floR gene (196).…”

Section: Resistance To Phenicolsmentioning

confidence: 99%

Antimicrobial Resistance inEscherichia coli

et al. 2018

View full text Add to dashboard Cite

Multidrug resistance in has become a worrying issue that is increasingly observed in human but also in veterinary medicine worldwide. is intrinsically susceptible to almost all clinically relevant antimicrobial agents, but this bacterial species has a great capacity to accumulate resistance genes, mostly through horizontal gene transfer. The most problematic mechanisms in correspond to the acquisition of genes coding for extended-spectrum β-lactamases (conferring resistance to broad-spectrum cephalosporins), carbapenemases (conferring resistance to carbapenems), 16S rRNA methylases (conferring pan-resistance to aminoglycosides), plasmid-mediated quinolone resistance (PMQR) genes (conferring resistance to [fluoro]quinolones), and genes (conferring resistance to polymyxins). Although the spread of carbapenemase genes has been mainly recognized in the human sector but poorly recognized in animals, colistin resistance in seems rather to be related to the use of colistin in veterinary medicine on a global scale. For the other resistance traits, their cross-transfer between the human and animal sectors still remains controversial even though genomic investigations indicate that extended-spectrum β-lactamase producers encountered in animals are distinct from those affecting humans. In addition, of animal origin often also show resistances to other-mostly older-antimicrobial agents, including tetracyclines, phenicols, sulfonamides, trimethoprim, and fosfomycin. Plasmids, especially multiresistance plasmids, but also other mobile genetic elements, such as transposons and gene cassettes in class 1 and class 2 integrons, seem to play a major role in the dissemination of resistance genes. Of note, coselection and persistence of resistances to critically important antimicrobial agents in human medicine also occurs through the massive use of antimicrobial agents in veterinary medicine, such as tetracyclines or sulfonamides, as long as all those determinants are located on the same genetic elements.

show abstract

“…Gelatinase production was assayed by inoculating test organisms on Gelatin agar and incubated at 37 • C for 24 h; the culture was flooded with mercuric chloride solution; thus, the development of opacity in the medium and a zone of clearance around the bacterial colonies were considered positive factors for the presence of gelatinase [70].…”

Section: Detection Of Virulence Factors Cytolysin (Hemolysin) and Gelmentioning

confidence: 99%

Antibiotic Resistance and Virulence Traits in Vancomycin-Resistant Enterococci (VRE) and Extended-Spectrum β-Lactamase/AmpC-producing (ESBL/AmpC) Enterobacteriaceae from Humans and Pets

et al. 2020

View full text Add to dashboard Cite

Background: We investigated the virulence factors, genes, antibiotic resistance patterns, and genotypes (VRE and ESBL/AmpC) production in Enterococci and Enterobacteriaceae strains isolated from fecal samples of humans, dogs, and cats. Methods: A total of 100 fecal samples from 50 humans, 25 dogs, and 25 cats were used in the study. MICs of nine antimicrobials were determined using the broth microdilution method. Polymerase chain reaction was used for the detection of genes responsible for antibiotic resistance (VRE and ESBL/AmpC) and virulence genes both in Enterococcus species, such as cytolysin (cylA, cylB, cylM), aggregation substance (agg), gelatinase (gelE), enterococcal surface protein (esp), cell wall adhesins (efaAfs and efaAfm), and in Enterobacteriaceae, such as cytolysin (hemolysin) and gelatinase production (afa, cdt, cnf1, hlyA, iutA, papC, sfa). Results: Enterococcus faecium was the most prevalent species in humans and cats, whereas Enterococcus faecalis was the species isolated in the remaining samples. A total of 200 Enterobacteriaceae strains were also detected, mainly from humans, and Escherichia coli was the most frequently isolated species in all types of samples. In the Enterococcus spp, the highest percentages of resistance for ampicillin, amoxicillin/clavulanate, erythromycin, tetracycline, ciprofloxacin, teicoplanin, and vancomycin were detected in cat isolates (41.6%, 52.8%, 38.9%, 23.6%, 62.5%, 20.8%, and 23.6% respectively), and in E. coli, a higher rate of resistance to cefotaxime and ceftazidime emerged in cat and dog samples, if compared with humans (75.4% and 66.0%, 80.0% and 71.4%, and 32.0% and 27.2%, respectively). Regarding the total number of enterococci, 5% and 3.4% of the strains were vancomycin and teicoplanin resistant, and the vancomycin resistance (van A) gene has been detected in all samples by PCR amplification. All the Enterobacteriaceae strains were confirmed as ESBL producers by PCR and sequencing, and the most frequent ESBL genes in E. coli strains from humans and pet samples were blaCTX-M-1 and blaCTX-M-15. Conclusions: Our results provide evidence that one or more virulence factors were present in both genera, underlining again the ability of pet strains to act as pathogens.

show abstract

Virulence factors, antibiotic resistance genes and genetic relatedness of commensal Escherichia coli isolates from dogs and their owners

Cited by 25 publications

References 22 publications

Detection of zoonotic diarrheagenic pathotypes of Escherichia coli in healthy household dogs

Detection of zoonotic diarrheagenic pathotypes of Escherichia coli in healthy household dogs

Antimicrobial Resistance inEscherichia coli

Antibiotic Resistance and Virulence Traits in Vancomycin-Resistant Enterococci (VRE) and Extended-Spectrum β-Lactamase/AmpC-producing (ESBL/AmpC) Enterobacteriaceae from Humans and Pets

Contact Info

Product

Resources

About