Virulence factors, antimicrobial resistance and phylogeny of bovine mastitis-associated<i>Streptococcus dysgalactiae</i>

Crippa, Bruna Lourenço; Rodrigues, Marjory Xavier; Tomazi, Tiago; Yang, Yongqiang; Rocha, Liliana de Oliveira; Silva, Nathália Cristina Cirone

doi:10.1017/s0022029923000195

Cited by 5 publications

(5 citation statements)

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“…This metagenomic bin clustered with the genomes of S.dysgalactiae subspecies dysgalactiae ( 73 ) obtained in two large-scale studies aimed at investigating the phylogeny and virulence factors of S.dysgalactiae strains associated with bovine mastitis. In these two studies, one in the United States ( 74 ) and the other in China (unpublished), 2 S. dysgalactiae strains were isolated from the milk of cows with mastitis. All other genomic sequences in this cluster except those assembled from the type material were also associated with bovine mastitis ( Supplementary Figure 4 ).…”

Section: Resultsmentioning

confidence: 99%

“…This bacterium has the potential to cause opportunistic infections in both animals and humans in various body sites ( 63 , 101 ). A thorough investigation of the microorganism’s phylogenetic position using MLST demonstrated that the identified strain belongs to the same cluster as the genomes of S.dysgalactiae obtained from cows’ milk with mastitis ( 74 ). This fact, along with abundant evidence linking S. dysgalactiae to mastitis ( 74 , 102 ), indicates that the mastitis symptoms described in certain capripoxvirus infections ( 103 ) are caused by this opportunistic pathogen.…”

Section: Discussionmentioning

confidence: 99%

“…A thorough investigation of the microorganism’s phylogenetic position using MLST demonstrated that the identified strain belongs to the same cluster as the genomes of S.dysgalactiae obtained from cows’ milk with mastitis ( 74 ). This fact, along with abundant evidence linking S. dysgalactiae to mastitis ( 74 , 102 ), indicates that the mastitis symptoms described in certain capripoxvirus infections ( 103 ) are caused by this opportunistic pathogen. The same can be said for another bacterium, Helcococcus ovis , associated with metritis in cattle and sheep ( 66 , 104 ).…”

Section: Discussionmentioning

confidence: 99%

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Metagenomic profiling of viral and microbial communities from the pox lesions of lumpy skin disease virus and sheeppox virus-infected hosts

Sharko,

Mazloum,

Krotova

et al. 2024

Front. Vet. Sci.

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IntroductionIt has been recognized that capripoxvirus infections have a strong cutaneous tropism with the manifestation of skin lesions in the form of nodules and scabs in the respective hosts, followed by necrosis and sloughing off. Considering that the skin microbiota is a complex community of commensal bacteria, fungi and viruses that are influenced by infections leading to pathological states, there is no evidence on how the skin microbiome is affected during capripoxvirus pathogenesis.MethodsIn this study, shotgun metagenomic sequencing was used to investigate the microbiome in pox lesions from hosts infected with lumpy skin disease virus and sheep pox virus.ResultsThe analysis revealed a high degree of variability in bacterial community structures across affected skin samples, indicating the importance of specific commensal microorganisms colonizing individual hosts. The most common and abundant bacteria found in scab samples were Fusobacterium necrophorum, Streptococcus dysgalactiae, Helcococcus ovis and Trueperella pyogenes, irrespective of host. Bacterial reads belonging to the genera Moraxella, Mannheimia, Corynebacterium, Staphylococcus and Micrococcus were identified.DiscussionThis study is the first to investigate capripox virus-associated changes in the skin microbiome using whole-genome metagenomic profiling. The findings will provide a basis for further investigation into capripoxvirus pathogenesis. In addition, this study highlights the challenge of selecting an optimal bioinformatics approach for the analysis of metagenomic data in clinical and veterinary practice. For example, direct classification of reads using a kmer-based algorithm resulted in a significant number of systematic false positives, which may be attributed to the peculiarities of the algorithm and database selection. On the contrary, the process of de novo assembly requires a large number of target reads from the symbiotic microbial community. In this work, the obtained sequencing data were processed by three different approaches, including direct classification of reads based on k-mers, mapping of reads to a marker gene database, and de novo assembly and binning of metagenomic contigs. The advantages and disadvantages of these techniques and their practicality in veterinary settings are discussed in relation to the results obtained.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Metagenomic profiling of viral and microbial communities from the pox lesions of lumpy skin disease virus and sheeppox virus-infected hosts

Sharko,

Mazloum,

Krotova

et al. 2024

Front. Vet. Sci.

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“…The analysis based on functional genes identified the presence of Streptococcus dysgalactiae and Streptococcus uberis in the pasteurized milk samples. Streptococcus dysgalactiae carries multiple virulence determinants and resistance genes, and can cause mastitis [43]. Streptococcus dysgalactiae infections can occur via several routes of transmission and may persist on dairy farms for more than one year [44].…”

Section: The Bacterial Community Composition In Pasteurized Milk Duri...mentioning

confidence: 99%

The Investigation of Changes in Bacterial Community of Pasteurized Milk during Cold Storage

Lan,

Wu,

et al. 2024

Foods

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The quality of pasteurized milk is commonly assessed through microbiological analysis, with variations in storage conditions significantly impacting the suppression of bacterial growth throughout the milk’s shelf life. This study investigated the dynamics of total bacterial counts (TBCs) and bacterial community shifts in milk that underwent pasteurization at 80 °C for 15 s. The milk was subsequently stored at 4 °C for varying intervals of 1, 4, 7, 10, 13, and 16 days. Culture-based testing revealed a significant TBC increase during the storage period spanning 1 to 16 days (up to −log10 4.2 CFU/mL at day 16). The TBC in pasteurized milk exhibited accelerated microbial growth from day 13 onwards, ultimately peaking on day 16. Bacillus was detected through 16S rRNA identification. Principal component analysis demonstrated a significant impact of storage time on bacterial communities in pasteurized milk. Analysis of bacterial diversity revealed a negative correlation between the Shannon index and the duration of pasteurized milk storage. Using high-throughput sequencing, Streptococcus and Acinetobacter were detected as prevalent bacterial genera, with Streptococcus dysgalactiae and Streptococcus uberis showing as dominant taxa. The presence of Streptococcus dysgalactiae and Streptococcus uberis in pasteurized milk might be attributed to the initial contamination from raw milk with mastitis. This study offers new evidence of the prevalence of bacterial community in pasteurized milk, thereby adding value to the enhancement of quality control and the development of strategies for reducing microbial risks.

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“…E. coli and Streptococcus are also major mastitis-causing pathogens in dairy cows. Multidrug-resistant (acquired resistance to ≥ three classes of antimicrobials) Streptococcus can be frequently isolated from raw milk of cows with mastitis, with the presence of antibiotic resistance genes and virulence genes ( 3 , 4 ).…”

mentioning

confidence: 99%

Editorial: Pathogenic and symbiotic bacteria in ruminants: antimicrobial resistance and microbial homeostasis

Yang,

Zhang,

El-Mahallawy

2024

Front. Vet. Sci.

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Virulence factors, antimicrobial resistance and phylogeny of bovine mastitis-associatedStreptococcus dysgalactiae

Cited by 5 publications

References 30 publications

Metagenomic profiling of viral and microbial communities from the pox lesions of lumpy skin disease virus and sheeppox virus-infected hosts

Metagenomic profiling of viral and microbial communities from the pox lesions of lumpy skin disease virus and sheeppox virus-infected hosts

The Investigation of Changes in Bacterial Community of Pasteurized Milk during Cold Storage

Editorial: Pathogenic and symbiotic bacteria in ruminants: antimicrobial resistance and microbial homeostasis

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