Virulence factors genes of Staphylococcus spp. isolated from caprine subclinical mastitis

Salaberry, Sandra Renata Sampaio; Saidenberg, André Becker Simões; Zuniga, Eveline; Melville, Priscilla Anne; Santos, Franklin Gerônimo Bispo; Guimarães, Ednaldo Carvalho; Gregori, Fábio; Benites, Nilson Roberti

doi:10.1016/j.micpath.2015.05.007

Cited by 29 publications

(17 citation statements)

References 31 publications

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“…To gain insight into the virulence profiles of the NAS species we also included all the publicly available annotated genome sequences of S. agnetis , S. chromogenes and S. simulans of veterinary origin and searched the putative virulence factors from these isolates ( Calcutt et al, 2013 ; Calcutt et al, 2014 ; Fry et al, 2014a ; Seixas et al, 2014 ; Al-Rubaye et al, 2015 ). Altogether, the virulence gene profiles of these ( Table S7 ) and our own isolates were similar to each other and profiles found in other studies ( Akineden et al, 2001 ; Srinivasan et al, 2006 ; Thomas et al, 2006 ; Kalorey et al, 2007 ; Sung, Lloyd & Lindsay, 2008 ; Hart, Hart & Roop, 2009 ; Oliveira et al, 2011 ; Wilson et al, 2011 ; Piessens et al, 2012 ; Simojoki et al, 2012 ; Tang et al, 2013 ; Tremblay et al, 2013 ; Nemeghaire et al, 2014 ; Salaberry et al, 2015 ; Snell et al, 2015 ; Xu et al, 2015 ; Adkins, Middleton & Fox, 2016 ; Artursson et al, 2016 ; Casaes Nunes et al, 2016 ; Kot et al, 2016 ; Mello et al, 2016 ). Some differences existed, however, mostly on the presence/absence of toxins.…”

Section: Discussionsupporting

confidence: 88%

Comparative genome analysis of 24 bovine-associatedStaphylococcusisolates with special focus on the putative virulence genes

Åvall-Jääskeläinen

Taponen

Kant

et al. 2018

PeerJ

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Non-aureus staphylococci (NAS) are most commonly isolated from subclinical mastitis. Different NAS species may, however, have diverse effects on the inflammatory response in the udder. We determined the genome sequences of 20 staphylococcal isolates from clinical or subclinical bovine mastitis, belonging to the NAS species Staphylococcus agnetis, S. chromogenes, and S. simulans, and focused on the putative virulence factor genes present in the genomes. For comparison we used our previously published genome sequences of four S. aureus isolates from bovine mastitis. The pan-genome and core genomes of the non-aureus isolates were characterized. After that, putative virulence factor orthologues were searched in silico. We compared the presence of putative virulence factors in the NAS species and S. aureus and evaluated the potential association between bacterial genotype and type of mastitis (clinical vs. subclinical). The NAS isolates had much less virulence gene orthologues than the S. aureus isolates. One third of the virulence genes were detected only in S. aureus. About 100 virulence genes were present in all S. aureus isolates, compared to about 40 to 50 in each NAS isolate. S. simulans differed the most. Several of the virulence genes detected among NAS were harbored only by S. simulans, but it also lacked a number of genes present both in S. agnetis and S. chromogenes. The type of mastitis was not associated with any specific virulence gene profile. It seems that the virulence gene profiles or cumulative number of different virulence genes are not directly associated with the type of mastitis (clinical or subclinical), indicating that host derived factors such as the immune status play a pivotal role in the manifestation of mastitis.

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Section: Discussionsupporting

confidence: 88%

Comparative genome analysis of 24 bovine-associatedStaphylococcusisolates with special focus on the putative virulence genes

Åvall-Jääskeläinen

Taponen

Kant

et al. 2018

PeerJ

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“…In 15 isolates of S. intermedius, obtained from cases of caprine subclinical mastitis, the most frequent genes encoding surface proteins involved in adhesion were laminin, elastin and fibrinogen binding proteins, in addition to one for fibronectin binding protein A (Salaberry et al 2015). Genes encoding collagen binding protein and fibronectin binding protein B were not detected, and the bap gene, encoding biofilm-associated proteins was observed in only 13% of isolates.…”

Section: Staphylococcus Intermedius Groupmentioning

confidence: 99%

“…In 15 S. intermedius isolated from subclinical mastitis, all genes encoding enterotoxins (A-E) were detected, with sec the most frequent (n ¼ 7) but only 6% (n ¼ 1) each of the others (Salaberry et al 2015). Due to their potential to cause vomiting and diarrhea, the presence of enterotoxins in non-aureus staphylococci could also be considered a risk in food-producing animals.…”

Section: Staphylococcus Intermedius Groupmentioning

confidence: 99%

Virulence factors in coagulase-positive staphylococci of veterinary interest other than Staphylococcus aureus

González-Martín

Corbera

Suárez‐Bonnet

et al. 2020

Veterinary Quarterly

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Coagulase-positive Staphylococci (CoPS) can exist as commensals in humans, companion and food-producing animals, but can cause severe or even lethal diseases. Exchange of these bacteria between humans and animals has been described. Special attention has been focused on Methicillin-Resistant Staphylococcus aureus, but other CoPS can also represent an important threat. In addition to significant antimicrobial resistance, these bacteria may carry a plethora of virulence factors-molecules that allow bacteria to establish on or within a host and increase their ability to cause disease. These virulence factors have been widely described in S. aureus but information about other species of CoPS is scarce. The aim of this paper is to review the recent literature about the virulence factors of non-aureus CoPS of animal origin. Their possible effects on human health are also described. The role and prevalence of different virulence factors including leukocidins, hemolysins, adhesins, enterotoxins, exfoliative and toxic shock syndrome toxins as well as superantigen-like proteins are addressed. The effect of these virulence factors on human health is also described. The possibility of misdiagnosis of species of CoPS has been demonstrated in human clinical samples. Prevalence of zoonotic infections could be higher than thought and medical laboratories should be aware of these other staphylococcal species. In keeping with the 'One Health' approach to animal and human disease, medical professionals, veterinarians and health workers should be aware of the risks derived from exposure to these bacteria in people in close contact with animals, including pet owners, farmers and veterinarians themselves.

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“…20 S. intermedius as infectious agent of domestic animals (mostly dogs, cats and pigeons) causes zoonotic infection in a wide range. 21,22 It is difficult to identify S. intermedius and S. pseudintermedius using conventional microbiological tests. Molecular diagnostic tools change the understanding of the epidemiology of these 2 organisms.…”

Section: The Status Of Zoonotic Staphylococcal Infectionsmentioning

confidence: 99%

Role of Enterotoxin-Producing Staphylococci in Zoonotic Infections in Iran

Ghasemian¹,

Nojoomi²,

Mahrooghi³

et al. 2018

Int J Enteric Pathog

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Context Typical opportunistic pathogens of coagulase positive Staphylococcus spp. are capable of causing a wide spectrum of different purulent and toxin-mediated diseases such as enteric infections. 1 Staphylococcus intermedius is very similar to S. aureus in phenotypic characteristics and similarly produces enterotoxins. 2-4 Pigment production, hemolysis, alkaline phosphatase and urease positive tests, mannitol and maltose fermentation and susceptibility to novobiocin and Polymyxin B are characteristics of S. intermedius. 2,5 Originally regarded as a single species, molecular characterization has placed S. intermedius in a new classification as S. intermedius group (SIG), which includes S. intermedius, S. pseudintermedius, and S. delphini. 6,7 These species cause diseases in human and animals (especially people who keep pets at home). S. aureus is a human commensal, while other coagulase positive species are present in animals. It has been revealed that S. intermedius mostly infects dogs (resides on the skin or mucosal surfaces). 8 The general transferability of these species between pets and humans has increased during recent years. 9,10 Staphylococcal species are able to express a wide spectrum of virulence factors, such as coagulase, cell wall components, hemolysins, proteases, enterotoxins, toxic-shock syndrome (TSS), and exfoliating toxins. 11 Enterotoxins produced by these species are pyrogenic components not inactivated by gastrointestinal enzymes and thermal process and lead to diarrhea. Enterotoxins are members of superantigens and more than 20 different types have been recognized, however some most important agents include SEA, SEB, SEC, SED and SEE. 12,13 On the other hand, increasing antibiotic resistance among coagulase positive staphylococci is an ongoing concern. 14 Differentiation of coagulase positive staphylococci except for S. aureus by phenotypic tests is a difficult and unreliable or insufficient process in laboratory settings. 15 Considering this, several methods have been introduced for the identification of these strains, 16 for example a molecular test including hsp60 and sodA genes could differentiate them. 17 S. intermedius is the predominant infectious agent among non-S. aureus coagulase positive species in Iran according to previous reports. The aims

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Virulence factors genes of Staphylococcus spp. isolated from caprine subclinical mastitis

Cited by 29 publications

References 31 publications

Comparative genome analysis of 24 bovine-associatedStaphylococcusisolates with special focus on the putative virulence genes

Comparative genome analysis of 24 bovine-associatedStaphylococcusisolates with special focus on the putative virulence genes

Virulence factors in coagulase-positive staphylococci of veterinary interest other than Staphylococcus aureus

Role of Enterotoxin-Producing Staphylococci in Zoonotic Infections in Iran

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