Virulence factors in Escherichia coli isolated from piglets with neonatal and post-weaning diarrhea in Japan

Nakazawa, Muneo; Sugimoto, Chihiro; Isayama, Y; Kashiwazaki, M

doi:10.1016/0378-1135(87)90060-5

Cited by 50 publications

(28 citation statements)

References 10 publications

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“…In this study, the percentage of ETEC + nondiarrheic piglets during the lactation period was 16.6%. Reported prevalence values in clinically affected neonates are higher, varying from 25.7% to 43.0% (Nakazawa et al, 1987;Do et al, 2006). However, the prevalence of ETEC + nursery pigs observed during this study does not differ from other reports involving animals with clinical diarrhea (Fairbrother et al, 2005;Do et al, 2006).…”

Section: Discussioncontrasting

confidence: 63%

EnterotoxigenicEscherichia coliSubclinical Infection in Pigs: Bacteriological and Genotypic Characterization and Antimicrobial Resistance Profiles

Moredo

Piñeyro

Márquez

et al. 2015

Foodborne Pathogens and Disease

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Enterotoxigenic Escherichia coli (ETEC) is the major pathogen responsible for neonatal diarrhea, postweaning diarrhea, and edema disease in pigs. Although it can be harmless, ETEC is also present in the intestines of other animal species and humans, causing occasional diarrhea outbreaks. The evaluation of this pathogen's presence in food sources is becoming an increasingly important issue in human health. In order to determine the prevalence of ETEC in nondiarrheic pigs, 990 animals from 11 pig farms were sampled. Using end-time polymerase chain reaction (PCR), eltA, estI genes, or both, were detected in 150 (15.2%) animals. From the positive samples, 40 (26.6%) ETEC strains were isolated, showing 19 antibiotic-resistance patterns; 52.5% of these strains had multiple antibiotic resistances, and 17.5% carried the intI2 gene. The most prevalent genotypes were rfb(O157)/estII/aidA (32.5%) and estI/estII (25.0%). The estII gene was identified most frequently (97.5%), followed by estI (37.5%), astA (20.0%), and eltA (12.5%). The genes coding the fimbriae F5, F6, and F18 were detected in three single isolates. The aidA gene was detected in 20 ETEC strains associated with the estII gene. Among the isolated ETEC strains, stx(2e)/estI, stx(2e)/estI/estII, and stx(2e)/estI/estII/intI2 genotypes were identified. The ETEC belonged to 12 different serogroups; 37.5% of them belonged to serotype O157:H19. Isolates were grouped by enterobacterial repetitive intergenic consensus-PCR into 5 clusters with 100.0% similarity. In this study, we demonstrated that numerous ETEC genotypes cohabit and circulate in swine populations without clinical manifestation of neonatal diarrhea, postweaning diarrhea, or edema disease in different production stages. The information generated is important not only for diagnostic and epidemiological purposes, but also for understanding the dynamics and ecology of ETEC in pigs in different production stages that can be potentially transmitted to humans from food animals.

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Section: Discussioncontrasting

confidence: 63%

EnterotoxigenicEscherichia coliSubclinical Infection in Pigs: Bacteriological and Genotypic Characterization and Antimicrobial Resistance Profiles

Moredo

Piñeyro

Márquez

et al. 2015

Foodborne Pathogens and Disease

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“…All animals were healthy and culture negative for EHEC strains as determined by a previously described technique [14] prior to inoculation. Pens had individual £oor drains and were cleaned twice daily with water and disinfectant.…”

Section: Experimental Animals and Inoculationmentioning

confidence: 99%

Clonal turnover of enterohemorrhagic Escherichia coli O157:H7 in experimentally infected cattle

Akiba

2000

FEMS Microbiology Letters

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A total of 401 enterohemorrhagic Escherichia coli (EHEC) O157:H7 isolates from two experimentally infected calves were analyzed using molecular biological methods. Genetic differences detected by pulsed‐field gel electrophoresis were observed between the inoculated and recovered strains as early as 1 day post inoculation. The loss of the inoculated clone was observed in one calf. Replication and dissemination of the EHEC O157:H7 strains that mutated in cattle may result in the diversification of this organism among cattle populations.

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“…8,9,16 The O-antigens of E. coli were identified by agglutination with polyclonal rabbit antisera as described. 24 The rabbit antisera were kindly provided by Dr. H. Hobayashi, National Institute of Animal Health, Tsukuba, Ibaraki, Japan. Clostridium perfringens was isolated on sheep blood agar and CW agar b incubated under anaerobiotic conditions for 24 hr at 37uC.…”

Section: Methodsmentioning

confidence: 99%

Frequency of Enteropathogen Detection in Suckling and Weaned Pigs with Diarrhea in Japan

et al. 2006

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Abstract. Fecal samples from suckling (n 5 153) and weaned (n 5 116) piglets with diarrhea in Japan were examined for shedding of viral, bacterial, and parasitic pathogens using culture, microscopic, and polymerase chain reaction methods. In suckling piglets, diarrhea was attributed to infection with a single etiologic agent in 60.8% of cases and with combinations of agents in 22.2%. In weaned piglets, diarrhea was attributed to a single etiologic agent in 43.1% and to combinations of agents in 47.4% of cases. Rotavirus was the most prevalent agent in suckling (67.3%) and weaned (65.5%) piglets. The detection of other pathogens was associated with age of the animals examined. Coccidia were predominantly isolated from suckling piglets, whereas Escherichia coli was found predominantly in weaned piglets. Although a relationship was not observed between detection rate of rotavirus and age of piglets, a single group of rotavirus was detected in 87.5% of suckling piglets whereas multiple groups were detected in 51.6% of weaned piglets. The results of this study confirm that diarrhea in piglets can, to a variable degree, be causally associated with multiple agents. Additionally, these results suggest reasons why this syndrome can be difficult to control.

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Virulence factors in Escherichia coli isolated from piglets with neonatal and post-weaning diarrhea in Japan

Cited by 50 publications

References 10 publications

EnterotoxigenicEscherichia coliSubclinical Infection in Pigs: Bacteriological and Genotypic Characterization and Antimicrobial Resistance Profiles

EnterotoxigenicEscherichia coliSubclinical Infection in Pigs: Bacteriological and Genotypic Characterization and Antimicrobial Resistance Profiles

Clonal turnover of enterohemorrhagic Escherichia coli O157:H7 in experimentally infected cattle

Frequency of Enteropathogen Detection in Suckling and Weaned Pigs with Diarrhea in Japan

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