Virulence factors of Mycoplasma synoviae: Three genes influencing colonization, immunogenicity, and transmissibility

Abstract

Infections caused by Mycoplasma synoviae are major welfare and economic concerns in poultry industries worldwide. These infections cause chronic respiratory disease and/or synovitis in chickens and turkeys leading to reduced production and increased mortality rates. The live attenuated vaccine strain MS-H (Vaxsafe® MS), commonly used for protection against M. synoviae infection in many countries, contains 32 single nucleotide variations compared to its wildtype parent strain, 86079/7NS. Genomic analysis of vaccine strains reisolated from flocks following the administration of MS-H has identified reversions to the original 86079/7NS sequence in the obgE, oppF and gapdh genes. Here, three MS-H field reisolates containing the 86079/7NS genotype in obgE (AS2), obgE and oppF (AB1), and obgE, oppF and gapdh (TS4), as well as the vaccine MS-H and the parental strain 86079/7NS were experimentally inoculated to chickens. The strains were assessed for their ability to infect and elicit immune responses in the recipient chickens, as well as in naïve in-contact chickens. Despite the loss of temperature sensitivity phenotype and colonization of the reisolates in the lower respiratory tract, there was no significant differences detected in the microscopic mucosal thickness of the middle or lower trachea of the inoculated chickens. Concurrent reversions in ObgE, OppF and GAPDH proteins were associated with higher gross air sac lesion scores and increased microscopic upper-tracheal mucosal thickness in chickens directly inoculated with the reisolates following intratracheal administration of a virulent strain of infectious bronchitis virus. The gross air sac lesions of the chickens in-contact with those inoculated with reisolates were not significantly different to those of chickens in-contact with MS-H inoculated chickens, suggesting that horizontal transmission of the reisolates in the poultry flock will not lead to higher pathogenicity or clinical signs. These results suggest a significant role of GAPDH and/or cumulative effect of ObgE, OppF and GAPDH on M. synoviae pathogenicity. Future experiments will be required to investigate the effect of single mutations in gapdh or oppF gene on pathogenicity of M. synoviae.

Unveiling genome plasticity and a novel phage inMycoplasma felis: genomic investigations of four feline isolates

“…Swabs were spread onto aerobic Sheep Blood Agar (SBA) plates and incubated at 37°C. Following observation and presumptive identification of small mycoplasma-like colonies, single colonies were transferred to Mycoplasma Broth (MB) containing 10% swine serum (Sigma-Australia) and 0.01% nicotinamide adenine dinucleotide (NAD) (Sigma-Australia) based on a formulation of Frey's medium with minor modification [27,28]. Acidification of MB media was used to confirm the growth of Mycoplasma spp.…”

“…Mycoplasmas are known for their rapid evolution and genetic plasticity, mainly driven by intraspecies spontaneous mutation or horizontal gene transfer (HGT) between and within species, facilitating swift adaptation to environmental changes and enhancing survival and/or virulence [6][7][8][9]. Studies examining the genomic stability of Mycoplasma synoviae revealed that it not only undergoes frequent mutations in birds [10], but also manifests rapid thermoadaptive evolution in vitro and in vivo [11], presumably to regain fitness and pathogenicity [12,13]. HGT is observed to occur more frequently between species that share an ecological niche irrespective of their phylogenetic divergence, such as transfer of surface VlhA lipoprotein involved in phase variation from Mycoplasma gallisepticum to M. synoviae infecting poultry [14], and transfer of several surface lipoproteins involved in host colonisation from Mycoplasma capricolum subsp.…”

“…Some mycoplasmas have been found to exhibit significant genetic plasticity and to evolve rapidly, mainly driven by intra-species spontaneous mutation and recombination or horizontal gene transfer (HGT) between and within species, facilitating swift adaptation to environmental changes, enhancing survival and/or virulence [6][7][8][9]. Studies examining the genomic stability of Mycoplasma synoviae have revealed that mutations occur frequently during infection in birds [10], and that rapid thermoadaptive evolution can occur in vitro and in vivo [11], presumably to regain fitness and pathogenicity [12,13]. HGT appears to occur more frequently between species that share an ecological niche, irrespective of their phylogenetic divergence.…”

Unveiling genome plasticity and a novel phage in Mycoplasma felis: Genomic investigations of four feline isolates

“…Some mycoplasmas have been found to exhibit significant genetic plasticity and to evolve rapidly, mainly driven by intra-species spontaneous mutation and recombination or horizontal gene transfer (HGT) between and within species, facilitating swift adaptation to environmental changes, enhancing survival and/or virulence [ 6 9 ]. Studies examining the genomic stability of Mycoplasma synoviae have revealed that mutations occur frequently during infection in birds [ 10 ], and that rapid thermoadaptive evolution can occur in vitro and in vivo [ 11 ], presumably to regain fitness and pathogenicity [ 12 13 ]. HGT appears to occur more frequently between species that share an ecological niche, irrespective of their phylogenetic divergence.…”

Hypothetical Proteins of Mycoplasma synoviae Reannotation and Expression Changes Identified via RNA-Sequencing

“…Dihydrolipoamide dehydrogenase, NADH oxidase, and the pyruvate dehydrogenase complex (PDC) E1 alpha and beta subunits of M. synoviae are all immunogenic, may bind the fibronectin/plasminogen protein, and are involved in the host adhesin process [9,10]. Oligopeptide (Opp) permease, recognized for its participation in humoral immune responses, can be researched as a potential candidate antigen [11]. NADH oxidase participates in M. synoviae adhesion to host cells can be studied as a diagnostic antigen and a potential protective vaccine candidate [6].…”