Virulence factors of the genus Fusarium with targets in plants

Gutiérrez-Sánchez, Angélica; Plasencia, Javier; Monribot-Villanueva, Juan L.; Rodríguez-Haas, Benjamín; Ruíz-May, Eliel; Guerrero-Analco, José A.; Sánchez-Rangel, Diana

doi:10.1016/j.micres.2023.127506

Cited by 8 publications

(1 citation statement)

References 224 publications

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“…After regulating soil conditions with optimized fertilization, this problem could be solved to some extent. The abundance of some common pathogenic groups in the treatment soil was significantly higher than that in control, such as Fusarium, one of the most important pathogens in ascomycetes, which often causes plant wilt in the soil of obstacle facilities [45]. The decline of this type of bacteria indicates that optimized fertilization can effectively reduce the damage of soil pathogens.…”

Section: Discussionmentioning

confidence: 94%

Optimized Fertilization Shifted Soil Microbial Properties and Improved Vegetable Growth in Facility Soils with Obstacles

Lang,

Ma,

Wang

et al. 2023

Horticulturae

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Currently, facility cultivation produces almost a third of all vegetables in China. The intensive production style has led to serious soil problems that need to be tackled. In this paper, a pot experiment was set up to evaluate the effects of optimized fertilization on vegetable growth and soil properties. Specifically, calcium, magnesium, boron and molybdenum were added on the basis of soil testing. The results showed that the growth of Chinese cabbage was significantly increased by optimized fertilization. The soil pH increased (by 3.82%), and EC decreased (by 8.54%). The abundance of culturable bacteria increased by 33.86%, whereas that of fungi decreased by 70.7%. The optimized fertilization increased the richness but not the evenness of soil microorganisms, increased the relative abundance of Proteobacteria, Firmicutes, Bacillus and Bacteroidetes, and decreased the relative abundance of Actinobacteria, Chloroflexi, and superphyla. Optimized fertilization inhibited the growth of Chytridiomycota and Mortierellomycota, especially the plant pathogen Fusarium. Moreover, balanced fertilization was beneficial in promoting various metabolic processes of soil bacteria. Soil water-soluble Ca, Mg, and available Mo might be the main factors driving the change in microbial groups.

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Section: Discussionmentioning

confidence: 94%

Optimized Fertilization Shifted Soil Microbial Properties and Improved Vegetable Growth in Facility Soils with Obstacles

Lang,

Ma,

Wang

et al. 2023

Horticulturae

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Metabolomics and dual proteomics identify contrasting patterns of major pathways affected in asparagus shoot upon Fusarium infection

Witzel,

Djalali Farahani-Kofoet,

Döll

et al. 2024

Plant Soil

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Aims Infections with soil-borne pathogens have considerable detrimental effects on asparagus (Asparagus officinalis) growth and production, notably caused by the Fusarium species F. oxysporum f.sp. asparagi, F. proliferatum, and F. redolens. To get insight into the systemic effects of fungal infection on plant physiology to identify candidate resistance traits, we investigated this interaction using a multi omics approach. Methods Asparagus plants were inoculated with one of the three Fusarium species. After 8 weeks, basal stem parts were harvested and subjected to metabolome and proteome analysis as well as detection of fungal DNA. Results Upon infection, the pathogen spreads systemically from the root to the shoot and, consequently, fungal DNA and mycotoxins were detected in the basal part of the plant stem. Metabolite data revealed that the main pathway affected by Fusarium infections was “Fatty acids”, specifically the superclasses “Glycerophospholipids”, “Glycerolipids” and “Sphingolipids” being lower abundant upon infection. Another main pathway identified in the analysis was “Shikimates and Phenylpropanoids” with compounds assigned to these classes being mainly enriched upon infection. Proteome data revealed an induction of pathogen-defense proteins upon infection in asparagus, while proteins involved in vesicle trafficking and lipid metabolism were lower abundant. Conclusions This indicates that not only lipid-based signaling processes are distorted by Fusarium, but also fundamental processes such as vesicle formation, membrane integrity and cell wall organization. In planta proteome analysis of F. oxysporum led to the identification of 1,488 fungal proteins, including proteins involved in metabolic and cellular processes as well as putative virulence factors.

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The secreted protein FonCHRD is essential for vegetative growth, asexual reproduction, and pathogenicity in watermelon Fusarium wilt fungus

Lou,

Wang,

Zeng

et al. 2024

Crop Health

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Fungal pathogens often secrete numerous effectors to interfere with and/or suppress plant immunity to promote their infection. Watermelon Fusarium wilt, caused by the soil-borne fungus Fusarium oxysporum f. sp. niveum (Fon), is one of the devastating diseases that severely affect the watermelon industry. Here, we report the function of a candidate effector protein, FonCHRD, in Fon. FonCHRD harbors a chordin (CHRD) domain of unknown function and has a signal peptide with secretion activity. FonCHRD shows a relatively high expression level in Fon marcoconidia and is inducible by watermelon root tissues. Phenotypic analysis of the targeted deletion mutant revealed that FonCHRD plays roles in vegetative growth, asexual reproduction, and conidial morphology of Fon, while it is not involved in spore germination as well as cell wall, oxidative and salt stress responses. Deletion of FonCHRD impaired the ability to colonize and spread within host plants, significantly reducing its virulence on watermelon. FonCHRD is distributed across multiple compartments of plant cells but can target to the apoplast space in plants. FonCHRD inhibits the INF1- and Bcl2-associated X protein-triggered cell death and defense gene expression in transiently expressed Nicotiana benthamiana leaves. These findings suggest that FonCHRD is essential for Fon pathogenicity by modulating invasive growth and spreading abilities as well as by suppressing plant immune responses.

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Virulence factors of the genus Fusarium with targets in plants

Cited by 8 publications

References 224 publications

Optimized Fertilization Shifted Soil Microbial Properties and Improved Vegetable Growth in Facility Soils with Obstacles

Optimized Fertilization Shifted Soil Microbial Properties and Improved Vegetable Growth in Facility Soils with Obstacles

Metabolomics and dual proteomics identify contrasting patterns of major pathways affected in asparagus shoot upon Fusarium infection

The secreted protein FonCHRD is essential for vegetative growth, asexual reproduction, and pathogenicity in watermelon Fusarium wilt fungus

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