Virulence genes and antimicrobial susceptibility in Pasteurella multocida isolates from calves

Katsuda, Ken; Hoshinoo, Kaori; Ueno, Yuichi; Kohmoto, Mariko; Mikami, Osamu

doi:10.1016/j.vetmic.2013.09.029

Cited by 55 publications

(58 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our study, a high frequency (80.68%) of tbpA was observed, suggesting significant association of this gene with small ruminant isolates. Between two proteins (HgbA and HgbB) involved in acquiring iron directly from the heme component, gene hgbA is present in almost all isolates, whereas prevalence of hgbB varies among strains of different host origin and also with disease status of the animal [1,28]. In contrast to the finding of Shayegh et al [13] on the low prevalence of hgbB gene among sheep isolates, our study revealed a higher prevalence (67%) of this gene.…”

Section: Discussioncontrasting

confidence: 87%

“…Filamentous haemagglutinins encoded by the pfhA gene play a major role in the initial colonization of the upper respiratory tract and the frequency of this gene varies greatly among strains of P. multocida. This gene has been reported to be an important epidemiological marker and correlated with occurrence of disease in cattle, swine and sheep [1,13,27,28]. Interestingly, we observed a very high prevalence of pfhA (95.4%) gene irrespective of its capsule type and origin of isolation.…”

Section: Discussionmentioning

confidence: 46%

“…This may be due to the preferential administration of some particular antibiotic by the veterinarians during treatment. Almost all previous studies reported that P. multocida recovered from different animals are susceptible to enrofloxacin, chloramphenicol, tetracycline and ciprofloxacin [7,8,28,33].…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Virulence gene profiling and antibiotic resistance pattern of Indian isolates of Pasteurella multocida of small ruminant origin

Sarangi

Thomas

Gupta

et al. 2015

Comparative Immunology, Microbiology and Infectious Diseases

View full text Add to dashboard Cite

Section: Discussioncontrasting

confidence: 87%

Section: Discussionmentioning

confidence: 46%

See 1 more Smart Citation

Virulence gene profiling and antibiotic resistance pattern of Indian isolates of Pasteurella multocida of small ruminant origin

Sarangi

Thomas

Gupta

et al. 2015

Comparative Immunology, Microbiology and Infectious Diseases

View full text Add to dashboard Cite

“…Some research suggested that the toxA gene is more significantly associated with serotype D, compared to serotype A [33,36] . We investigated iron-regulated and Characteristics Analysis of P. multocida Strains iron-acquisition related genes (tonB, fur, hgbB, hgbA) irrespective of the animal clinical status and found that all isolates had the tonB gene similar to the findings reported previously [37] . In a past study [38] , the tonB gene encoded an energy transfer protein.…”

Section: Discussionsupporting

confidence: 65%

Çin’de Kanatlı Hayvanlardan İzole Edilen Pasteurella multocida Suşlarının Moleküler Karakterizasyonu ve tonB Geni Açısından Genetik Analizi

Li¹,

Cheng²,

Lan³

et al. 2017

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

Pasteurella multocida (P. multocida) causes fowl cholera in birds and the pathogenesis and virulence factors involved are still poorly understood. TonB protein is a periplasmic protein prevalent in a large number of Gram-negative bacteria, as in Pasteurella, which is believed to be responsible for the import of ferric iron complexes across the outer membrane and plays a role as an important virulence factor to help bacteria to obtain nutrients. In this study, a total of 23 isolates of P. multocida were obtained from 172 cases of clinical respiratory disease in duck and geese, giving an isolation rate of 13.4%. P. multocida Serogroup A was isolated from 22 cases (95.7%), whereas serogroup F was from 1 case (4.3%). All isolates were analyzed for their susceptibility to 15 antibiotics and the presence of 18 genes for virulence factors. The susceptibility profiles suggested that Tetracycline, Cephalosporin, Enrofloxacin, and Aminoglycosides were the drugs most likely to be active against P. multocida. However, 78.3% and 52.2% of poultry strains were resistant to Penicillin and Sulfisoxazole, respectively. PCR results showed that ptfA, fimA, tonB, fur, hgbA, hgbB, sodA, sodC, pmHAS, nanH, nanB, plpB and ompH genes occur in most poultry strains of P. multocida. The virulence genes such as toxA, tadD, hsf-1, pfhA and ompA were each present in <73.9% of strains. The tonB gene was detected in all 23 clinical strains of P. multocida which were recovered from different hosts and harboring different serotypes. Phylogenetic analysis indicated that these tonB genes of P. multocida from different hosts and harboring different serotypes have close genetic relationship with a high similarity. Multiple sequence alignment demonstrated that TonB protein of Gram-negative bacteria exists in multiple conserved sites, such as -xSSGx-, -YP-, -LD-and xA[A/V]Lx motif. These findings provide clinical data into the epidemiological and molecular characteristics of avian P. multocida isolates and provide a reference for the researches of drug target in P. multocida. Keywords: Antimicrobial susceptibility, Capsular antigens, Pasteurella multocida, tonB gene, Virulence gene, Cloning, Phylogenetic analysis Çin'de Kanatlı Hayvanlardan İzole Edilen Pasteurella multocida Sışlarının Moleküler Karakterizasyonu ve tonB Geni Açısından Genetik Analizi ÖzetPasteurella multocida (P. multocida) kuşlarda kanatlı kolerasına neden olur ve hastalıkta virulans faktörleri hala tam olarak anlaşılamamıştır. TonB proteini Pasteurella da dahil pek çok Gram-negatif bakteride yaygın olarak bulunan periplazmik bir protein olup membran dışına ferrik demir komplekslerini taşımakta görevlidir ve bakterilerde besin sağlamaya yardım etmek suretiyle önemli bir virulans faktörü olarak rol oynar. Bu çalışmada, 172 klinik solunum hastalıklı ördek ve kazdan toplam 23 P. multocida izolatı elde edilerek %13.4'lük izolasyon oranı sağlandı. P. multocida Serogrup A 22 vakadan izole edilirken (%95.7), serogrup F 1 vakadan izole edildi (%4.3). Tüm izolatlar 15 antibiyotiğe kar...

show abstract

“…Comparison of the Common ... components [7][8][9][10][11] . The common serological methods, based on capsular and somatic antigen, are; indirect haemagglutination (IHA) test for capsular serotypes detection and agglutination/agar gel immunodiffusion (AGID) tests for somatic serotyping.…”

Section: Introductionmentioning

confidence: 99%

Pasteurella multocida B:2 ve B:3,4 Serotiplerinin Ortak İmmunojenik Protein Komponentlerinin Karşılaştırılması

Ghani¹,

Khattak²,

Saqlain³

et al. 2016

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

Serotype B of Pasteurella multocida is a major agent of haemorrhagic septicaemia (HS), a form of blood poisoning widespread in cattle and buffalo from Southern Asia. The vaccines currently used against the disease are not potent enough, being effective only for a limited duration only. In an attempt to identify immunogenic components of P. multocida for use as live/subunit vaccines, the outer membrane proteins (OMPs) of B:2 and B:3,4 serotypes were isolated and their profiles compared. Three common immunogenic components; polypeptides of molecular weight (MW) 39.0, 33.5, 31.0 kDa, were identified by immunoblotting. The immunogenicity of identified OMPs was also confirmed using antisera generated against them. The antigenic OMPs identified in current research could be explored in the formulation of new, safe and effective vaccines for indigenous buffalo and cattle breeds with a prolonged efficacy. Keywords: Cattle, OMP, Vaccine, Haemorrhagic septicemia Pasteurella multocida B:2 ve B:3,4 Serotiplerinin Ortak İmmunojenik Protein Komponentlerinin Karşılaştırılması ÖzetPasteurella multocida B serotipi Güney Asya'daki sığır ve mandalarda yaygın olarak görülen ve kanda septisemi ile seyreden hemorajik septisemi (HS)'nin en önemli nedenidir. Hastalık için günümüzde kullanılan aşılar yeteri kadar etkili değildir ve sadece sınırlı bir sürede etkisini göstermektedir. Canlı/subunit aşılarda kullanılmak üzere Pasteurella multocida'nın immunojenik komponentlerinin belirlenmesi için yapılan çalışmamızda, B:2 ve B:3,4 serotiplerinin dış membran proteinleri (OMP) izole edilmiş ve bunların profilleri karşılaştırılmıştır. İmmunbloting sonucunda 39.0, 33.5 ve 31.0 kDa moleküler ağırlıklarında 3 adet ortak immunojenik polipeptit belirlenmiştir. İdentifiye edilen dış membran proteinlerinin immunojenitesi ayrıca bunlardan elde edilen antiserumlar ile doğrulanmıştır. Bu çalışmada identifiye edilen antijenik OMP'ler uzun süre etki gösteren yeni, güvenli ve etkin aşıların formülasyonlarının geliştirilmesine katkı sağlayacaktır.

show abstract

Virulence genes and antimicrobial susceptibility in Pasteurella multocida isolates from calves

Cited by 55 publications

References 12 publications

Virulence gene profiling and antibiotic resistance pattern of Indian isolates of Pasteurella multocida of small ruminant origin

Virulence gene profiling and antibiotic resistance pattern of Indian isolates of Pasteurella multocida of small ruminant origin

Çin’de Kanatlı Hayvanlardan İzole Edilen Pasteurella multocida Suşlarının Moleküler Karakterizasyonu ve tonB Geni Açısından Genetik Analizi

Pasteurella multocida B:2 ve B:3,4 Serotiplerinin Ortak İmmunojenik Protein Komponentlerinin Karşılaştırılması

Contact Info

Product

Resources

About